Determination of gamma-hydroxybutyric acid in serum and urine by headspace solid-phase dynamic extraction combined with gas chromatography–positive chemical ionization mass spectrometry

Lenz, Daniel; Kröner, Lars; Rothschild, Markus A.

doi:10.1016/j.chroma.2009.03.011

Cited by 26 publications

(9 citation statements)

References 37 publications

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“…[22][23][24][25][26][27] To the best of our knowledge, there is only one report published to date describing a pharmacokinetic study using the HS-SPDE-GC-MS/ MS approach. Conventional pre-treatment methods such as liquid-liquid extraction (LLE) used for quantifying the concentration of compounds in biological samples can cause signicant evaporative losses of the volatile components, which are hard to enrich, resulting in the loss of sensitivity and unacceptable assay accuracy.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of multiple volatile active components in rat plasma using a headspace-solid phase dynamic extraction method coupled to gas chromatography-tandem mass spectroscopy: application in a pharmacokinetic study of Longhu Rendan pills

et al. 2015

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Longhu Rendan pills (LRPs), a traditional Chinese over-the-counter medicine, have been used for the prevention and treatment of heatstroke and motion sickness. A sensitive, specific, and accurate headspace-solid-phase dynamic extraction method coupled to gas chromatography-tandem mass spectrometry (HS-SPDE-GC-MS/MS) was developed and validated for the investigation of the pharmacokinetic properties of L-menthol, borneol, isoborneol, and the metabolite camphor in rats after oral administration of LRPs. Target compounds were extracted using an SPDE needle device coated with a polydimethylsiloxane solid phase. Detection of components was achieved by GC-MS/MS in multiple reaction monitoring mode. This method was successfully applied in the evaluation of the pharmacokinetics of components and a metabolite of LRPs after a single intragastric administration of a 0.92 g kg À1 dose to rats. Pharmacokinetic parameters were calculated from the plasma concentrationtime data. C max values of L-menthol, borneol, isoborneol, and camphor in rat plasma were determined to be 876 AE 341, 268 AE 149, 158 AE 91, and 126 AE 56 ng mL À1 , respectively, and the AUC 0-t values were measured as 876 AE 259, 408 AE 121, 140 AE 50, and 401 AE 35 ng h mL À1 , respectively. These results provide useful information on the effective components of LRPs.

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Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of multiple volatile active components in rat plasma using a headspace-solid phase dynamic extraction method coupled to gas chromatography-tandem mass spectroscopy: application in a pharmacokinetic study of Longhu Rendan pills

et al. 2015

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“…SPME can be used as direct immersion of the fiber into the sample to extract the analytes, or introduction of the fiber in the sample headspace to extract volatile compounds that are partitioned between gaseous and liquid phases. Many factors, such as pH, temperature, salt concentration, and stirring, affect the equilibrium constant and equilibration time [7–16]. …”

Section: Introductionmentioning

confidence: 99%

Solid Phase Microextraction and Related Techniques for Drugs in Biological Samples

Moein

Said

Bassyouni

et al. 2014

Journal of Analytical Methods in Chemistry

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In drug discovery and development, the quantification of drugs in biological samples is an important task for the determination of the physiological performance of the investigated drugs. After sampling, the next step in the analytical process is sample preparation. Because of the low concentration levels of drug in plasma and the variety of the metabolites, the selected extraction technique should be virtually exhaustive. Recent developments of sample handling techniques are directed, from one side, toward automatization and online coupling of sample preparation units. The primary objective of this review is to present the recent developments in microextraction sample preparation methods for analysis of drugs in biological fluids. Microextraction techniques allow for less consumption of solvent, reagents, and packing materials, and small sample volumes can be used. In this review the use of solid phase microextraction (SPME), microextraction in packed sorbent (MEPS), and stir-bar sorbtive extraction (SBSE) in drug analysis will be discussed. In addition, the use of new sorbents such as monoliths and molecularly imprinted polymers will be presented.

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“…Many previously reported analytical methods detect GHB in different biological matrices and involve the use of gas chromatography (GC) [25][26][27], but also liquid chromatography (LC) [28,29] and capillary zone electrophoresis (CZE) [30]. Also the determination of GHB through headspace solid phase micro extraction (SPME) and dynamic extraction (SPDE) has recently been published [31,32].…”

Section: Troductiomentioning

confidence: 99%

Determination of gamma-hydroxybutyric acid in dried blood spots using a simple GC-MS method with direct “on spot” derivatization

2010

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Determination of gamma-hydroxybutyric acid in serum and urine by headspace solid-phase dynamic extraction combined with gas chromatography–positive chemical ionization mass spectrometry

Cited by 26 publications

References 37 publications

Simultaneous quantification of multiple volatile active components in rat plasma using a headspace-solid phase dynamic extraction method coupled to gas chromatography-tandem mass spectroscopy: application in a pharmacokinetic study of Longhu Rendan pills

Simultaneous quantification of multiple volatile active components in rat plasma using a headspace-solid phase dynamic extraction method coupled to gas chromatography-tandem mass spectroscopy: application in a pharmacokinetic study of Longhu Rendan pills

Solid Phase Microextraction and Related Techniques for Drugs in Biological Samples

Determination of gamma-hydroxybutyric acid in dried blood spots using a simple GC-MS method with direct “on spot” derivatization

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