Determination of glial fibrillary acidic protein (GFAP) in human brain tumors

Jacque, C.; Vinner, C.; Kujas, M; Raoul, M.; Racadot, J; Baumann, Nicole

doi:10.1016/0022-510x(78)90107-7

Cited by 190 publications

(104 citation statements)

References 10 publications

Supporting

Mentioning

100

Contrasting

Order By: Relevance

“…Also astrocytomas contained raised but very variable amounts of GFA. It has been proposed that GFA concentration in gliomas might be related to the degree of astrocytic differentiation, and hence to the grade of tumour malignancy (Jacque et al, 1978). Our results do not support this hypothesis.…”

Section: Resultscontrasting

confidence: 99%

“…In concordance with Jacque et al (1978) we found high GFA contents in a craniopharyngioma and a haemangioblastoma. Although tumours of non-glial origin do not normally allow infiltration by reactive astroglia, the craniopharyngioma and haemangioblastoma in our material most certainly contained astrocytic elements.…”

Section: Resultssupporting

confidence: 85%

“…In addition GFA has been demonstrated in cross-reacting forms in brains of a variety of vertebrates including birds and fish (Dahl & Bignami, 1973). Increased levels of GFA in glioblastomas were first demonstrated in our laboratory (Dittmann et al, 1977) and this finding was later verified by others (Delpech et al, 1978;Jacque et al, 1978).…”

mentioning

confidence: 59%

See 2 more Smart Citations

Quantitation of glial fibrillary acidic protein in human brain tumours

Rasmussen¹,

Bock²,

Warecka³

et al. 1980

Br J Cancer

View full text Add to dashboard Cite

Summary.-The glial fibrillary acidic protein (GFA) content of 58 human brain tumours was determined by quantitative immunoelectrophoresis, using monospecific antibody against GFA. Astrocytomas, glioblastomas, oligodendrogliomas, spongioblastomas, ependymomas and medulloblastomas contained relatively high amounts of GFA, up to 85 times the concentration in parietal grey substance of normal human brain. GFA was not found in neurinomas, meningiomas, adenomas of the hypophysis, or in a single case of metastasis of adenocarcinoma. Non-glial tumours of craniopharyngioma and haemangioblastoma were infiltrated by reactive astroglia and showed considerable amounts of GFA.

show abstract

Section: Resultscontrasting

confidence: 99%

Section: Resultssupporting

confidence: 85%

See 1 more Smart Citation

Quantitation of glial fibrillary acidic protein in human brain tumours

Rasmussen¹,

Bock²,

Warecka³

et al. 1980

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…Increased GFAP expression is an indication of the up-regulation of this protein, which usually occurs when brain tissues undergo injury or at diseased state [54]. Slight increased expression of GFAP, which is an intermediate filament protein of astrocytes and ependymal cells [55][56][57], is indicative of the traumatic effect of RV. Marked GFAP expression is also indicative of reactive astrogliosis [58], which may be detrimental on the long run as it usually underlie neural dysfunction and pathology in certain neurological disease states [59].…”

Section: Discussionmentioning

confidence: 99%

Rauwolfia vomitoria and Gongronema latifolium extracts influences cerebellar cortex

Ekong¹,

Nwakanma²

2017

Alzheimers Dement Cogn Neurol

View full text Add to dashboard Cite

Oxidative stress and free radical production is an etiology to some neurodegenerative diseases which may be preventable by prior neuronal protection using herbs. Rauwolfia vomitoria and Gongronema latifolium are medicinal herbs with antioxidant, anti-diabetic and analgesic properties among others. While R. vomitoria acts as a brain stimulant, as well as a depressant, neurotoxic effects have also been reported, which G. latifolium has shown the potential to mitigate. This study therefore investigated the effects of the combination of R. vomitoria and G. latifolium on young rats' cerebellar cortex. Twenty young male Wistar rats (100-150 g) were divided equally into 4 groups (n=5). Oral doses of the vehicle (Tween 20™), ethanolic extracts of 200 mg/kg of R. vomitoria (RV), 200 mg/kg of G. latifolium (GL), and the combination of both (RV + GL) were given to the animals for 14 days. On day 15, the animals were sacrificed after ketamine sedation and perfusion-fixed with 10% buffered-formalin. The cerebella were excised and processed for histomorphology by silver impregnation technique and immunolabelled with anti-neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP). The histology results showed atrophied Purkinje and other neurons with increased NSE and GFAP expressions in the RV group, which were not as such in the GL and RV+GL groups, an indication of cerebellar cortical injury. In conclusion, RV was injurious to the cerebellar cortical neurons and also stimulated NSE and GFAP, but these RV-induced traumas were slightly mitigated with GL combination. This preliminary report of RV+GL combination may be considered an alternative to RV single treatment for better disease management and brain protection.

show abstract

“…Glial fibrillary acidic protein (GFAP) is an intermediate filament protein that is expressed by cells of the central nervous system and generally considered to be specific for astrocytes [19,20] . GFAP has been described in non neural tissues including liver [21][22][23] .…”

Section: Introductionmentioning

confidence: 99%

Karbon Tetraklorür ile oluşturulan Hepatotoksisite Karaciğer Paranşimindeki Nitrik oksit ve Gliyal Fibriller Asidik Protein (GFAP) Sunumları

Dinçel¹,

Atasever²,

Yaman³

2016

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Carbon tetrachloride (CCl4)-induced liver injury causes necrosis and fibrosis, which may lead to cirrhosis and liver failure. Although a lot of study to understand the mechanism of liver fibrosis, our knowledge of the molecular level of this disease is still incomplete. The aim of this study was to investigate the effect and regulation of nitric oxide (NO) and glial fibrillary acidic protein (GFAP) on the severity of hepatic injury in male Wistar albino rats with CCl4-induced hepatotoxicity and elucidate the underlying mechanism. A total of 20 male rats were randomly divided into healthy control group (A) and CCl4-induced hepatic fibrosis model group (B), with 10 rats in each group. The results of this study suggest CCl4-induced hepatic injury is mediated by excessive NO production and up-regulated by inducible and endothelial NO synthase (P<0.05), which may plays a role in increasing hepatic injury. Additionally, liver injury induced by CCl4 demonstrated significant increases in GFAP (P<0.05). Histological and immunohistochemical analyses of the CCl4-treated group exhibited increased inflammatory process and liver necrosis/fibrosis. Inflammatory response especially NO and GFAP expression is identified to play an important role in the development of liver injury and fibrosis induced by CCl4. Keywords: Carbon tetrachloride, Nitric oxide, Glial fibrillary acidic protein, Fibrosis Karbon Tetraklorür İle Oluşturulan Hepatotoksisite Karaciğer Paranşimindeki Nitrik Oksit ve Gliyal Fibriller Asidik Protein (GFAP) Sunumları ÖzetKarbon tetraklorür (CCl4) karaciğerde nekroz ve fibrozis meydana getirerek siroza ve karaciğer yetmezliğine neden olabilmektedir. Günümüzde karaciğer fibrozis mekanizmasının anlaşılmasına yönelik çok fazla çalışma olmasına rağmen, oluşum mekanizması ile ilgili moleküler düzeydeki bilgiler hala eksiktir. Bu çalışmada CCl4 ile hepatotoksisite oluşturulan erkek Wistar albino sıçanlarda fibrozisin oluşum aşamasında nitric oksit (NO) ve glial fibriller asidik protein (GFAP) etkilerinin araştırılması ve altında yatan mekanizmanın aydınlatılması amaçlanmıştır. Çalışmada 20 adet erkek sıçan, her grupta toplam 10 sıçan olmak üzere sağlıklı kontrol grubu (A) ve CCl4 ile oluşturulan karaciğer fibrozu grubu (B) olarak rastgele ikiye ayrıldı. Histolojik ve immunohistokimyasal analizlerle CCl4 grubunda yangısal değişiklikler ile karaciğer nekroz/fibrozis tespit edildi. On iki hafta süreyle CCl4 verilerek oluşturulan karaciğer hasarının oluşumunda, indüklenebilen ve endotelyal NO sentazın (P<0.05) yüksek sunumlarına bağlı olarak artan NO üretiminin önemli bir rol oynadığı belirlendi. Ayrıca, CCl4 grubunda karaciğerde GFAP (P<0.05) sunumlarında önemli artış da gösterildi. Sonuç olarak, CCl4 kaynaklı karaciğer hasarı ve fibrozisin gelişiminde NO ve GFAP'ın önemli bir rol oynadığı tespit edildi.

show abstract

Determination of glial fibrillary acidic protein (GFAP) in human brain tumors

Cited by 190 publications

References 10 publications

Quantitation of glial fibrillary acidic protein in human brain tumours

Quantitation of glial fibrillary acidic protein in human brain tumours

Rauwolfia vomitoria and Gongronema latifolium extracts influences cerebellar cortex

Karbon Tetraklorür ile oluşturulan Hepatotoksisite Karaciğer Paranşimindeki Nitrik oksit ve Gliyal Fibriller Asidik Protein (GFAP) Sunumları

Contact Info

Product

Resources

About