Determination of Glibenclamide By Analytical Spectrophotometry

Abstract

Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λmax = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R2 = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively. First derivative spectrophotometric (1DS) method was applied for the determination of (GB) in the presence (MET). (GB) was determined at 317 nm (1D317). Linearity ranges were (4 – 240) μg/mL for (GB). Regression analysis showed a good correlation coefficients R2 = 0.999914. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.60 μg/mL and 1.83 μg/mL for (GB). The proposed zero spectrophotometry method was applied to analysis individual (GB), and the derivative (1D317) method was applied to analysis (GB) individually or combined with (MET) in Syrian trademark drugs. The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.

Development of a method for the quantitative determination of glibenclamide in tablets

“…For spectrophotometric methods in the visible and UV spectrum, there is a need to optimize the conditions for increasing selectivity, such as by using derivatization reagents or changing the pH of the medium [14][15][16].…”

“…Among them, chromatographic and spectrophotometric in the UV and visible range of the spectrum are mainly found. Most often, glibenclamide in tablets and biological fluids is determined by TLC with densitometry [3], high-performance thin-layer chromatography [4,5], liquid chromatography [6], reversed-phase liquid chromatography [7], tandem method of liquid chromatography with mass spectrometry [8], HPLC [9], OF-HPLC [10,11], combined methods of HPLC with spectrophotometry [12,13], spectrophotometric methods [14][15][16] and UV-visible spectroscopy [17].…”