Determination of glucosamine and galactosamine using borate buffers for modification of the Elson-Morgan and Morgan-Elson reactions

Good, Thomas A.; Bessman, Samuel P.

doi:10.1016/0003-2697(64)90183-6

Cited by 96 publications

(25 citation statements)

References 14 publications

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“…The cell wall (10 mg) was hydrolyzed in a sealed tube with 6N-distilled HCl (10 ml), at 110°C for 24 h [16]. Glucosamine was estimated in the hydrolysate [17]. Calibration curve N-acetyl glucosamine.…”

Section: Cell Wall Analysismentioning

confidence: 99%

Extractability of polyhydroxyalkanoate synthesized by Bacillus flexus cultivated in organic and inorganic nutrient media

Divyashree

Shamala

2010

Indian J Microbiol

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Abstractwas isolated from local soil nutrient medium (IM) containing sucrose as carbon source, yield of biomass and polyhydroxyalkanoate (PHA) were 2 g/l and 1 g/l (50% of biomass), respectively. Substitution of inorganic nitrogen by peptone, yeast extract or beef extract resulted in biomass yields of 4.1, 3.9 and 1.6 g/l, respectively. Corresponding yields of PHA in biomass was 30%, 40% and 44%. Cells subjected to change in nutrient condition from organic to inorganic, lacked diaminopimelic acid in the cell wall and the concentration of amino acids also decreased. Under these conditions the extractability of the polymer from the cells by hot chloroform or mild alkali hydrolysis was 86-100% compared to those grown in yeast extract or peptone (32-56%). The results demonstrated that growth, PHA production and the composition of cell wall of present in the growth medium. Cells grown in inorganic medium lysed easily and this can be further exploited for easier recovery of the intracellular PHA.

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Section: Cell Wall Analysismentioning

confidence: 99%

Extractability of polyhydroxyalkanoate synthesized by Bacillus flexus cultivated in organic and inorganic nutrient media

Divyashree

Shamala

2010

Indian J Microbiol

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“…Respective controls were prepared according to Khale and Deshpande (1992). The methods of Good and Bessman (1964) and Reissig et al (1955) were followed for total glucosamine and N-GlcNAc.…”

Section: Cell Wall Analysismentioning

confidence: 99%

The extracellular constitutive production of chitin deacetylase in Metarhizium anisopliae: possible edge to entomopathogenic fungi in the biological control of insect pests

Nahar¹,

Ghormade²,

Deshpande³

2004

Journal of Invertebrate Pathology

103

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“…When methylumbelliferyl derivatives were used, free 4-methylumbelliferone was measured as previously described. Free N-acetylglucosamine was estimated by the method of Good and Bessman [33]. Results of enzymic hydrolysis were expressed as a percentage of the total N-acetylhexosamine.…”

Section: Substrate Specijicity Studiesmentioning

confidence: 99%

Properties of Four Molecular Forms of N-Acetyl-beta-d-Hexosaminidase Isolated from Germinating Seeds of Fenugreek (Trigonella foenum graecum)

Bouquelet

Spik

1978

Eur J Biochem

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Four molecular forms of N‐acetyl‐β‐d‐hexosaminidase located in different parts of the germinating fenugreek seeds have been characterized and isolated. Their activities vary during the germination process. The optimal pH and temperature as well as Km value towards synthetic substrates were found to be different for the N‐acetyl‐β‐d‐glucosaminidase and N‐acetyl‐β‐d‐galactosaminidase activities of each molecular form. Both N‐acetyl‐β‐d‐glucosaminidase and N‐acetyl‐β‐d‐galactosaminidase activities of molecular form I increased to a similar extent in the presence of Fe2+ ions whereas both activities of molecular forms II, III and IV were depressed. In the presence of Fe3+ ions, only the N‐acetyl‐β‐d‐galactosaminidase activity of molecular form I increased. No inhibition of either activity of N‐acetyl‐hexosaminidase form I was obtained in the presence of 5 μM HgCl2 or p‐chloromercuribenzoate, whereas both activities of the other forms were inhibited. These four N‐acetyl‐hexosaminidases hydrolyse synthetic and natural substrates: hexosaminidase form I liberates the 1,4‐β‐linked N‐acetyl‐glucosamine residue, form II liberates the 1,6‐linked residue at a very slow rate, whereas forms III and IV liberate 1,2‐β‐linked N‐acetylglucosamine. On isoelectric focusing, pI values of 6.78, 6.3, 4.9 and 4.65 were determined for the forms I, II, III and IV respectively. Using Sepharose 6B filtration, molecular weights were estimated to be 84 000 ± 10 000, 72 000 ± 10 000, 180 000 ± 10 000 and 150 000 ± 10 000 for the forms I, II, III and IV respectively. Treatment with dodecyl sulfate resulted in the dissociation of all four forms with bands having a molecular weight of 28 000 ± 2000, 24 000 ± 2000, 30 500 ± 2000 and 30 000 ± 2000 for the forms I, II, III and IV respectively. These results suggest that the four hexosaminidases may be polymers formed by association of between three and six subunits of identical molecular weight. Immunological studies reveal common antigenic determinants between forms I and II located in cotyledon and endosperm and between forms III and IV located predominantly in the embryo.

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Determination of glucosamine and galactosamine using borate buffers for modification of the Elson-Morgan and Morgan-Elson reactions

Cited by 96 publications

References 14 publications

Extractability of polyhydroxyalkanoate synthesized by Bacillus flexus cultivated in organic and inorganic nutrient media

Extractability of polyhydroxyalkanoate synthesized by Bacillus flexus cultivated in organic and inorganic nutrient media

The extracellular constitutive production of chitin deacetylase in Metarhizium anisopliae: possible edge to entomopathogenic fungi in the biological control of insect pests

Properties of Four Molecular Forms of N-Acetyl-beta-d-Hexosaminidase Isolated from Germinating Seeds of Fenugreek (Trigonella foenum graecum)

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