Determination of Glucose in Dried Serum Samples by Fourier-Transform Infrared Spectroscopy

Abstract

Background: Practical improvements are needed to allow measurement of glucose concentrations by Fourier- transform infrared (FT-IR) spectroscopy. We developed a new method that allows determination of the glucose concentration in dried sera. Methods: We studied 32 serum samples after fourfold dilution and desiccation before FT-IR analyses on a spectrometer operated at a resolution of 2.0 cm−1. We integrated all spectral windows at the surface of the spectrum in the C—O region. For comparison, glucose was measured in the sera by a glucose oxidase method. Results: One peak within the spectrum was most specific for glucose (997–1062 cm−1). Its surface integration showed a strong relationship with reference data (r = 0.998; P <0.001). FT-IR analyses of five glucose solutions were performed to determine its specific absorption at the same peak. In this way, glucose concentrations in serum spectra could be measured. For the first time while using FT-IR spectroscopy, no manipulation of spectra nor use of internal standard was necessary to obtain results in high accordance with glucose concentration measured by a conventional (glucose-oxidase) method (Sy|x = 0.25 mmol/L; r = 0.998). Conclusions: FT-IR spectroscopy appears to be an easy and accurate method to determine glucose concentration and could be widely used to simultaneously identify and quantify several metabolites in biological fluids or tissues.

Quantitative IR microscopy and spectromics open the way to 3D digital pathology

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Screening the low molecular weight fraction of human serum using ATR-IR spectroscopy

“…Variations in their concentrations can be a good indicator to detect undergoing pathological processes, and therefore as physiological markers for rapid diagnostics. Glucose remains the most common example, but also one of most targeted molecules for the development of ex vivo quantitative models, notably IR spectroscopy . While the normal average concentration for glucose is roughly about 0.8 mg/mL , making its detection achievable in whole serum, monitoring and estimation of its blood levels based on spectral features will be significantly limited in the presence of highly abundant proteins (HMWF).…”

FT‐IR spectrometry analysis of plasma fatty acyl moieties selective mobilization during endurance exercise

“…Although the basic structure of any fatty acid is not complex, the polymorphism of FAM (esterified or not, saturated, mono or polyunsaturated, and with chain length varying from 4 to 24 carbons) is a challenge for analytical techniques, which might determine each species concentration before global information becomes available. Therefore, with the possibility of determining a wide range of plasma concentrations,16, 18, 24, 25 structural characteristics of FAM are available from the same plasma spectra, notably for chain length and unsaturation level, through the ν CCH, ν as CH 2 , and ν as CH 3 absorptions. This allowed us to analyze concomitant (and possibly correlated) changes between plasma molecular concentrations and FAM structural characteristics.…”

“…FT‐IR spectrometry (Bruker, IFS 28/B, Germany) was used for plasma FT‐IR spectra acquisition as previously described 24. On plasma FT‐IR spectra, glucose, lactate, glycerol, TG, albumin, and FAM concentrations were determined as previously described 16–18, 24, 25. Plasma volume change (PVC) between rest and exercise was also calculated using plasma FT‐IR spectra 23…”