Determination of glutathione and glutathione disulfide using zone fluidics and fluorimetric detection

Tsiasioti, Apostolia; Tzanavaras, Paraskevas D.

doi:10.1016/j.talanta.2020.121559

Cited by 22 publications

(8 citation statements)

References 31 publications

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“…Glutathione (GSH) is a tripeptide compound produced by the liver that is composed of three different amino acids: cysteine, glutamic acid and glycine (ƴ-Glu-Cys-Gly) [ 1 ]. GSH acts as an antioxidant and is involved in many processes in the body, including tissue building and repair, cysteine transport and storage, signal transduction modulation, cell propagation control and immune system response regulation [ 2 , 3 ]. Abnormal levels of GSH have been reported to contribute to oxidative stress, which plays a key role in aging, and in a variety of diseases, including cancer, neurotoxicity, psoriasis, liver damage, Parkinson’s disease, and Alzheimer’s disease.…”

Section: Introductionmentioning

confidence: 99%

“Turn on” Fluorescence Sensor of Glutathione Based on Inner Filter Effect of Co-Doped Carbon Dot/Gold Nanoparticle Composites

Kim

Park

2021

IJMS

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Glutathione (GSH) is a thiol that plays a significant role in nutrient metabolism, antioxidant defense and the regulation of cellular events. GSH deficiency is related to variety of diseases, so it is useful to develop novel approaches for GSH evaluation and detection. In this study we used nitrogen and phosphorus co-doped carbon dot-gold nanoparticle (NPCD–AuNP) composites to fabricate a simple and selective fluorescence sensor for GSH detection. We employed the reductant potential of the nitrogen and phosphorus co-doped carbon dots (NPCDs) themselves to form AuNPs, and subsequently NPCD–AuNP composites from Au3+. The composites were characterized by using a range of spectroscopic and electron microscopic techniques, including electrophoretic light scattering and X-ray diffraction. The overlap of the fluorescence emission spectrum of NPCDs and the absorption spectrum of AuNPs resulted in an effective inner filter effect (IFE) in the composite material, leading to a quenching of the fluorescence intensity. In the presence of GSH, the fluorescence intensity of the composite was recovered, which increased proportionally to increasing the GSH concentration. In addition, our GSH sensing method showed good selectivity and sensing potential in human serum with a limit of detection of 0.1 µM and acceptable results.

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Section: Introductionmentioning

confidence: 99%

“Turn on” Fluorescence Sensor of Glutathione Based on Inner Filter Effect of Co-Doped Carbon Dot/Gold Nanoparticle Composites

Kim

Park

2021

IJMS

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“…Probably, in higher pH (>8), due to the formation of GSSG species, the competitive interaction for attraction of the Cu 2+ ions interrupts and affects the fluorescence recovery efficiency. 44,45 Effects of several amino acids together with BSA and urea on the as-designed "turn-off" probe were also investigated for the selectivity test. As shown in Figure 3B, significant recovery toward GSH was observed, while other species nearly had no or very low interference effect.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In this case, we expect deprotonation of sulfur groups in GSH to occur in this pH, leading to the higher affinity of GS – to the Cu 2+ ions. Probably, in higher pH (>8), due to the formation of GSSG species, the competitive interaction for attraction of the Cu 2+ ions interrupts and affects the fluorescence recovery efficiency. , …”

Section: Resultsmentioning

confidence: 99%

Bright Green Light-Emitting Gold Nanoclusters Confined in Insulin as Selective Fluorescent Switch Probes for Sensing and Imaging of Copper Ions and Glutathione

Shamsipur

Babaee

Gholivand

et al. 2023

ACS Appl. Nano Mater.

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In this study, an on−off−on fluorescence probe is developed for rapid, sensitive, and selective detection of Cu 2+ ions and glutathione (GSH) based on green lightemitting insulin-capped gold nanoclusters (Ins/Au-NCs). Ins/Au-NCs with low toxicity and excellent stability were prepared by utilizing a simple and green strategy. Cu 2+ ions can selectively decrease the fluorescence intensity of Ins/Au-NCs by an aggregation-caused quenching mechanism, which can be recovered in the presence of GSH. Under the optimized condition, the detection limits for determination of Cu 2+ ions and GSH are estimated as 7.5 and 9.2 nM, with linear ranges of 0.05 to 1.7 μM and 0.05 to 1.5 μM, respectively. Chemical modeling was also employed to investigate the interaction of the Ins/Au-NCs probe with Cu 2+ , Hg 2+ , glutathione, and cysteine. The calculated molecular orbital energy well confirmed the experimental selective results obtained. Luminescence cellular imaging exhibited a sensitive response of the Ins/Au-NCs probe toward Cu 2+ ions in breast cancer cells MCF-7, and thus, it potentially acted as an intracellular GSH probe in MCF-7 compared to the human fibroblast HFF cells. The asprepared probe was successfully applied to low levels of Cu 2+ ions and GSH in water and human serum samples. The designed Ins/ Au-NCs-based probe may also provide a biocompatible platform for label-free GSH detection in biological and clinical implications that comprise staining and early prevention of cancer, AIDS, and Alzheimer's and cardiovascular diseases.

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“…At the same time, as the most important antioxidant in the body, it can effectively protect proteins, DNA and other biomolecules in cells from loss by oxidation. 1 GSH in cells is converted from reduced glutathione to oxidized glutathione (GSSG) when biological tissues are stimulated by oxidation. This results in altered levels of oxidized and reduced glutathione in the body, which affects cell growth and function.…”

Section: Instructionmentioning

confidence: 99%

Turn-on signal fluorescence sensor based on DNA derived bio-dots/polydopamine nanoparticles for the detection of glutathione

Chen

et al. 2022

RSC Adv.

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Determination of glutathione and glutathione disulfide using zone fluidics and fluorimetric detection

Cited by 22 publications

References 31 publications

“Turn on” Fluorescence Sensor of Glutathione Based on Inner Filter Effect of Co-Doped Carbon Dot/Gold Nanoparticle Composites

“Turn on” Fluorescence Sensor of Glutathione Based on Inner Filter Effect of Co-Doped Carbon Dot/Gold Nanoparticle Composites

Bright Green Light-Emitting Gold Nanoclusters Confined in Insulin as Selective Fluorescent Switch Probes for Sensing and Imaging of Copper Ions and Glutathione

Turn-on signal fluorescence sensor based on DNA derived bio-dots/polydopamine nanoparticles for the detection of glutathione

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