Determination of Hepatoprotective and Antioxidant Role of Walnuts Against Ethanol-Induced Oxidative Stress in Rats

Abstract

The aims of our study were the evaluation of the hepatoprotective effect and antioxidant role of walnuts against ethanol-induced oxidative stress. The hepatoprotective and antioxidant role of the walnuts supplementation feed against ethanol-induced oxidative stress were evaluated by measuring liver damage serum marker enzymes; aspartate aminotransferase (AST); alanine aminotransferase (ALT); gamma glutamyl transpeptidase (GGT); lactate dehydrogenase (LDH); and antioxidant defense systems such as reduced glutathione, glutathione reductase, superoxide dismutase, glutathione-S-transferase, catalase, and glutathione peroxidase and Malondialdehyde (MDA) content in various tissues of rats. Rats were divided into six experimental groups: I (control), II (20 % ethanol), III (10 % walnuts), IV (20 % ethanol + 10 % walnuts), V (5 % walnuts), and VI (20 % ethanol + 5 % walnuts). According to the results, the biochemical analysis showed a considerable increase in the serum aspartate AST, ALT, GGT, and LDH in the group II as compared to that of group I, whereas decreased in group IVas compared to that of group II. In addition, administration of walnuts supplementation restored the ethanol-induced imbalance between MDA and fluctuated antioxidant system toward close control group particularly in the tissues. The results indicated that walnuts could be as important as diet-derived antioxidants in preventing oxidative damage in the tissues by reducing the lipid oxidation or inhibiting the production of ethanol-induced free radicals in rats.

Potential immunomodulatory and antioxidant effects of walnut Juglans regia vegetable oil against lead‐mediated hepatic damage and their interaction with lipase activity in rats

“…Conversely, our results demonstrate that JRVO protect from oxidative damage by stabilizing the enzyme levels (AST, ALT, ALP, LDH) indicating a protective role for this oil against liver dysfunctions. Similar findings were reported earlier upon Juglans extract but not Juglans oil against CCl4‐induced hepatotoxicity or against ethanol‐induced hepatotoxicity in rats …”

“…Similar findings were reported earlier upon Juglans extract but not Juglans oil against CCl4-induced hepatotoxicity 8 or against ethanol-induced hepatotoxicity in rats. 46 Lipids play an important role in hepatic disease incidence because they form cell membranes and several hormones in healthy bodies. In our study, administration of lead acetate was found to cause significant elevation in TG, TC, LDL cholesterol levels, TC/HDL-C, LDL-C/ HDL-C ratio, with a concomitant decrease of HDL-C attributed to dyslipidaemia, as well as increase of lipase activity.…”

Does Oxidative Stress Induced by Alcohol Consumption Affect Orthodontic Treatment Outcome?

“…It is well known that aldehydes and ROS can directly affect both proteins or DNA, leading to transcription-repression of concrete genes. In fact, the role of ROS and aldehydes seems to be a key factor for these alterations, partially confirmed by the fact that administration of antioxidants prevents these EtOH-induced cellular alterations (Herrera et al, 2003; Bati et al, 2015; Han et al, 2015). …”

Antioxidant properties of Komagataeibacter hansenii CGMCC 3917 and its ameliorative effects on alcohol-induced liver injury in mice

“…Group Ⅰ (n =10, normal group): mice were treated by gavage with normal saline at a dose of 10 mL/kg twice a day with an interval under 2 h. Group Ⅱ (n =10, alcohol-treated group): mice received 28% (v/v) ethanol (10 mL/kg BW) 2 h after normal saline by intragastric gavage (10 mL/kg BW). Groups III (n =10) and IV (n =10) are dose-dependent test groups, where mice were administered by intragastric gavage with 28% (v/v) ethanol (10 mL/ kg BW) 2 h after administration of CGMCC 3917 bacterial suspension by intragastric gavage at a dose of 10 mL/kg BW (Bati, Celik, & Dogan, 2014). The concentrations of bacterial suspension were approximately 2 ×10 8 (highdose group) and 2 ×10 6 CFUs (low-dose group), respectively.…”

“…The activities of AST, ALT, and ALP in the serum are considered as reliable and sensitive indicators of ALD (Bati et al, 2014;Uyanoglu et al, 2014). AST and ALT are enzymes present in hepatocytes, and these intracellular enzymes are released into the circulation when injury is induced in the liver (Wang et al, 2014).…”