Determination of Hesperidin and Monoglucosylhesperidin Contents in Processed Foods Using Relative Molar Sensitivity Based on &lt;sup&gt;1&lt;/sup&gt;H-Quantitative NMR

Nishizaki, Yuzo; Sato-Masumoto, Naoko; Nakanishi, Akihito; Hashizume, Yushi; Tandia, Mahamadou; Yamazaki, Taichi; Kuroe, Miho; Numata, Masahiko; Ihara, Toshihide; Sugimoto, Naotoshi; Sato, Kyoko

doi:10.3358/shokueishi.59.1

Cited by 12 publications

(6 citation statements)

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“…The next stage beyond the direct characterization of organic standard solutions will be direct determination of analytes in actual samples with a primary standard. The qNMR/chromatography (and maybe the post-column reaction GC) can be utilized to realize a direct link from the analytical results of actual samples such as foods to a single traceability source [70,71]. However, to obtain accurate analytical results, problems such as low recovery through pretreatment (extraction and cleanup) steps and incomplete removal of contaminants that causes the matrix effect should be solved.…”

Section: Discussionmentioning

confidence: 99%

Conventional and new traceability schemes of organic standards for safe water supply in Japan

et al. 2019

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Metrological traceability to the International System of Units (SI) is required to ensure reliability of analytical results. For this purpose, SI-traceable standard solutions of respective analytes are essential for common analytical methods such as chromatography. However, it is not so easy to fulfill demands due to the existence of a vast number of targets, especially in the case of organic analysis. Because high-purity organic materials which are raw materials of standard solutions must be characterized by compound-independent methods, the mass balance approach (subtraction method) and the primary methods of measurement (e.g. the freezing point depression method and titrimetry) are used. Recently, quantitative NMR (qNMR) has been popularized.

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Section: Discussionmentioning

confidence: 99%

Conventional and new traceability schemes of organic standards for safe water supply in Japan

et al. 2019

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“…The determination temperature was set at room temperature (20-30 °C). We performed 13 C decoupling with MPF8. The dummy scan was performed twice, and the scan was performed 64 times.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, Sugimoto and colleagues have developed a singlereference HPLC method with relative molar sensitivity (RMS) determined by off-line combination of 1 H-quantitaive NMR ( 1 H-qNMR) and LC/photo diode array (PDA) for quantitative analysis of analytes in natural products. [12][13][14][15][16] A single reference standard is used as an internal standard in the 1 H-qNMR and HPLC methods to calculate the RMS values of each tar-geted analyte in various samples. These values can be determined as analyte-specific factors from the response ratios obtained by HPLC and the molar ratios obtained by 1 H-qNMR.…”

Section: Introductionmentioning

confidence: 99%

Miroestrol Quantification in <i>Pueraria mirifica</i> Crude Drugs and Products by Single-Reference UPLC/PDA/MS Using Relative Molar Sensitivities to Kwakhurin

Masada¹,

Hosoe²,

Arai³

et al. 2021

CHEMICAL & PHARMACEUTICAL BULLETIN

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Owing to occasional health damages caused by health food products derived from Pueraria mirifica (PM), the Japanese government has designated PM as an "ingredient calling for special attention." Miroestrol is a specific isoflavone isolated from PM and possesses very strong estrogenic activity enough to induce side effects in small amount. Therefore, routine analyses for miroestrol quantification is recommended to control the safety and quality of PM products. However, miroestrol content in PM is quite low, and commercial reagent for its detection is rarely available. In this study, we developed a quantitative analysis method for miroestrol in PM without using its analytical standard by using the relative molar sensitivity (RMS) of miroestrol to kwakhurin, another PM-specific isoflavone, as a reference standard. The RMS value was obtained by an offline combination of 1 H-quantitative NMR spectroscopy and a LC/photo diode array (PDA) and miroestrol content was determined by single-reference LC/PDA using RMS. Furthermore, we investigated miroestrol content in commercially available PM crude drugs and products, and the RMS method was compared with the conventional calibration curve method in terms of performance. The rate of concordance of miroestrol contents determined by two method was 89-101%. The results revealed that our developed LC/PDA/MS method with RMS using kwakhurin as a reference standard was accurate for routine monitoring of miroestrol content in PM crude drugs and products to control their quality.

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“…Furthermore, qNMR/chromatography is applicable to determine organic compounds in complex matrices such as natural products and food [8,9]. Since the supply of reference materials for every potential analyte is impossible, this method is useful to establish traceability for organic analyses widely.…”

Section: Chromatographic Response Factor Assisted Qnmr 2121extended I...mentioning

confidence: 99%

Advanced approaches and applications of qNMR

Huang

Zhang

et al. 2020

Metrologia

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Quantitative nuclear magnetic resonance (qNMR), as a primary approach used to characterize SI (International System of Units)-traceable organic compounds, is based on the proportionality of intensities from analyte and standard, which implies a fundamental requirement that the peaks to be used for quantitation are isolated from impurities. Therefore, sufficient dispersion or a data analysis method has to be used to enable isolation of the peaks for quantitation of impurities. This technique offers many metrological advantages, though significant challenges associated with factors such as calibration reference materials, experimental parameter optimization and isolation of quantitative peaks must be considered to ensure confidence in the results. This review focuses on the development of advanced qNMR methods (including combined qNMR methods), especially those that enhance measurement selectivity and mitigate biases associated with chemical interferences, aiming to satisfy this requirement. For this aim, in recent years, different advanced qNMR approaches have been developed to remove interferences spatially, mathematically, spectroscopically, etc. The principles, advantages, challenges and future prospects of these approaches are introduced in this review. These advanced approaches aim to improve qNMR accuracy for analytes with molecular weight up to ~6000 g/mol. The approaches are aimed at removing potential systematic errors in qNMR to improve its trueness and its application as primary metrolgical method and routine analysis method.

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Determination of Hesperidin and Monoglucosylhesperidin Contents in Processed Foods Using Relative Molar Sensitivity Based on <sup>1</sup>H-Quantitative NMR

Cited by 12 publications

References 0 publications

Conventional and new traceability schemes of organic standards for safe water supply in Japan

Conventional and new traceability schemes of organic standards for safe water supply in Japan

Miroestrol Quantification in <i>Pueraria mirifica</i> Crude Drugs and Products by Single-Reference UPLC/PDA/MS Using Relative Molar Sensitivities to Kwakhurin

Advanced approaches and applications of qNMR

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