Determination of hydrogen peroxide in sea water by flow-injection analysis with chemiluminescence detection

Real‐time detection of reactive oxygen species generation by marine phytoplankton using flow injection—chemiluminescence

“…The optimum chemiluminescence response for H 2 O 2 was recorded at pH 10.8 using a 100 nM standard prepared in FSW. This is consistent with the pH optimum reported by Price et al (1994Price et al ( , 1998) using a system with separate luminol and Co(II) reagent streams.…”

“…H 2 O 2 was measured by the metal [Co(II)] catalyzed oxidation of luminol (adapted from Price et al 1994). Modifications were made to the original method; the sample injection loop was reduced to 60 µL, and the previously separate reagents were mixed to a single combined luminol/Co(II) reagent solution (Fig.…”

Chemiluminescence on a Microchip

“…Luminol and TCPO were dissolved in 0.1 M Na2CO3 buffer solution (pH 10.2) 21 and ethyl acetate, respectively. Their concentrations were 10 -4 M. Fluorescent dye perylene was added in the TCPO solution, its concentration was 10 -4 M. A stock solution of 10 -2 M H2O2 was made by diluting 31% H2O2 with water.…”

Kinetic Flow-injection Determination of Hydrogen Peroxide by Use of Iron(III)-catalyzed Coloration and Its Application to the Determination of Biological Substances

“…The need for rapid and simple methods capable of determining trace amounts of the peroxide is increasing in environmental, clinical and food analyses. A number of manual and flow-injection (FI) methods have been reported for the determination of hydrogen peroxide by using enzymatic and nonenzymatic reactions combined with photometric, [3][4][5][6][7] fluorometric, [8][9][10][11][12] chemiluminescent [13][14][15][16][17] and electrochemical 18,19 detections. Especially, FI procedures including immobilized enzyme reactors, which lead to the generation of hydrogen peroxide, have provided attractive methods for specific determinations of clinically interesting substances, such as glucose, cholesterol and glutamic acid.…”