Determination of hydroxylated polychlorinated biphenyls by offline solid-phase extraction-liquid chromatography–tandem mass spectrometry using a molecularly imprinted polymer as a sorbent for sample preconcentration

Abstract

a b s t r a c tHydroxylated polychlorinated biphenyls (OH-PCBs) can be detected by liquid chromatography-mass spectrometry coupled to solid-phase extraction (SPE) using a dummy molecularly imprinted polymer (DMIP) as a sorbent. The DMIP is prepared by using an analogue of OH-PCBs (i.e., 4, 4-dihydroxybiphenyl) as a dummy template, to avoid the leakage of the target molecules. The DMIP-SPE sorbent shows good recoveries for OH-PCBs at pH 11 due to the charge-assisted hydrogen bondings between OH-PCBs and the DMIP. It has been found that the DMIP is much more effective and selective than the traditional C 18 -SPE method. The sample pH, polymer dosage, elution solvent and volume have been optimized for higher recoveries. Under the optimum experimental conditions, OH-PCBs can be detected in the linear concentration range of 0.05-1.0 pM, with the detection limits ranging from 11 fM to 82 fM for 4′-OH-CB 9, 4′-OH-CB 30, 4′-OH-CB 61, 4′-OH-CB 106 and 4′-OH-CB 112. The proposed system has been successfully applied to the determination of trace OH-PCBs in spiked water samples with recoveries in the range of 89-110%.

Aptamer‐Functionalized Magnetic Conjugated Organic Framework for Selective Extraction of Traces of Hydroxylated Polychlorinated Biphenyls in Human Serum

“…The recovery of OH-PCBs was calculated with Equation (4): [31] Recovery ¼ where C i and C f are the initial and final concentrations of OH-PCBs in the sample solution, and V i and V f are the volumes of the solution before and after the extraction process, respectively.…”

“…[30,31] In humans and animals, PCBs can be metabolizedt oh ydroxylated PCBs (OH-PCBs) by cytochrome P450-mediated oxidation. [30,31] In humans and animals, PCBs can be metabolizedt oh ydroxylated PCBs (OH-PCBs) by cytochrome P450-mediated oxidation.…”

“…[33,34] Pilehvar et al developed as ensing system for the detection of OH-PCBsi nh uman serum by employing an electrode modified with aptamer-immobilized carbon nanotubes. [31,32] Herein, we report the synthesis of aptamer-functionalized magnetic core-shell COF materials. Compared with electrochemical methods, LC-MS exhibits higher sensitivity and has become am ore powerful analytical technique for the determination of OH-PCBs.…”

“…Polychlorinated biphenyls (PCBs), an otorious class of persistent organic pollutants (POPs), have contributed to global environmental pollution and caused great harm duet ot heir high toxicitya nd bioaccumulation. [30,31] In humans and animals, PCBs can be metabolizedt oh ydroxylated PCBs (OH-PCBs) by cytochrome P450-mediated oxidation. [32] At present,t here are some reports on the determination of OH-PCBs in human blood and tissue samples.…”

Determination of Aroclor 1254 in Water Samples Using Polystyrene–Divinylbenzene Solid-Phase Extraction and Gas Chromatography

“…In addition, sample pH can affect the binding interactions including hydrogen bonding and ionic interactions between the PS-DVB polymers and analytes, which influence the adsorption efficiency of SPE [15,20]. As shown in Fig.…”