Determination of <i>S‐</i>Adenosyl‐<scp>l</scp>‐methionine in Fruits by Capillary Electrophoresis

Poel, Bram Van de; Bulens, Inge; Lagrain, Pieter; Pollet, Jeroen; Hertog, Maarten; Lammertyn, Jeroen; Proft, M.P. De; Nicolaı̈, Bart

doi:10.1002/pca.1241

Cited by 25 publications

(19 citation statements)

References 26 publications

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“…For each maturity stage and each tissue type, all metabolites (SAM, ACC and MACC) and enzyme activities (ACO and ACS) from the ethylene biosynthesis pathway were quantified. SAM was extracted and quantified by capillary electrophoresis (P/ACE-MDQ, Beckmann Coulter, Fullerton, CA, USA) in a glycine : phosphate buffer (300 : 50 mM, pH 2.5) as described by [51]. ACC and MACC content was measured exactly as described by [50].…”

Section: Methodsmentioning

confidence: 99%

Tissue specific analysis reveals a differential organization and regulation of both ethylene biosynthesis and E8 during climacteric ripening of tomato

et al. 2014

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BackgroundSolanum lycopersicum or tomato is extensively studied with respect to the ethylene metabolism during climacteric ripening, focusing almost exclusively on fruit pericarp. In this work the ethylene biosynthesis pathway was examined in all major tomato fruit tissues: pericarp, septa, columella, placenta, locular gel and seeds. The tissue specific ethylene production rate was measured throughout fruit development, climacteric ripening and postharvest storage. All ethylene intermediate metabolites (1-aminocyclopropane-1-carboxylic acid (ACC), malonyl-ACC (MACC) and S-adenosyl-L-methionine (SAM)) and enzyme activities (ACC-oxidase (ACO) and ACC-synthase (ACS)) were assessed.ResultsAll tissues showed a similar climacteric pattern in ethylene productions, but with a different amplitude. Profound differences were found between tissue types at the metabolic and enzymatic level. The pericarp tissue produced the highest amount of ethylene, but showed only a low ACC content and limited ACS activity, while the locular gel accumulated a lot of ACC, MACC and SAM and showed only limited ACO and ACS activity. Central tissues (septa, columella and placenta) showed a strong accumulation of ACC and MACC. These differences indicate that the ethylene biosynthesis pathway is organized and regulated in a tissue specific way. The possible role of inter- and intra-tissue transport is discussed to explain these discrepancies. Furthermore, the antagonistic relation between ACO and E8, an ethylene biosynthesis inhibiting protein, was shown to be tissue specific and developmentally regulated. In addition, ethylene inhibition by E8 is not achieved by a direct interaction between ACO and E8, as previously suggested in literature.ConclusionsThe Ethylene biosynthesis pathway and E8 show a tissue specific and developmental differentiation throughout tomato fruit development and ripening.

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Section: Methodsmentioning

confidence: 99%

Tissue specific analysis reveals a differential organization and regulation of both ethylene biosynthesis and E8 during climacteric ripening of tomato

et al. 2014

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“…MACC was obtained after acidic hydrolysis according to Hoffman et al (1982) and also recently updated by Bulens et al (2011). SAM was extracted and quantified by capillary electrophoresis (P/ ACE MDQ, Beckman Coulter) with UV detection according to Van de Poel et al (2010). Met content was measured by gas chromatography-mass spectrometry (Agilent) as described by Oms-Oliu et al (2011).…”

Section: Ethylene and Metabolite Quantificationmentioning

confidence: 99%

Targeted Systems Biology Profiling of Tomato Fruit Reveals Coordination of the Yang Cycle and a Distinct Regulation of Ethylene Biosynthesis during Postclimacteric Ripening

et al. 2012

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The concept of system 1 and system 2 ethylene biosynthesis during climacteric fruit ripening was initially described four decades ago. Although much is known about fruit development and climacteric ripening, little information is available about how ethylene biosynthesis is regulated during the postclimacteric phase. A targeted systems biology approach revealed a novel regulatory mechanism of ethylene biosynthesis of tomato (Solanum lycopersicum) when fruit have reached their maximal ethylene production level and which is characterized by a decline in ethylene biosynthesis. Ethylene production is shut down at the level of 1-aminocyclopropane-1-carboxylic acid oxidase. At the same time, 1-aminocyclopropane-1-carboxylic acid synthase activity increases. Analysis of the Yang cycle showed that the Yang cycle genes are regulated in a coordinated way and are highly expressed during postclimacteric ripening. Postclimacteric red tomatoes on the plant showed only a moderate regulation of 1-aminocyclopropane-1-carboxylic acid synthase and Yang cycle genes compared with the regulation in detached fruit. Treatment of red fruit with 1-methylcyclopropane and ethephon revealed that the shut-down mechanism in ethylene biosynthesis is developmentally programmed and only moderately ethylene sensitive. We propose that the termination of autocatalytic ethylene biosynthesis of system 2 in ripe fruit delays senescence and preserves the fruit until seed dispersal.

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“…In addition, two diastereoisomers of this compound exist, known as (S,S)-and (R,S)-S-adenosyll-methionine, among which only one has demonstrated to be biologically active (S,S-). Van de Poel et al [69] demonstrated that SAM content varies depending on food processing (relationship between heat treatment and SAM content in tomato samples is shown in Fig. 7(A,B)).…”

Section: Other Amino Acidsmentioning

confidence: 89%

“…Four degradation compounds (degradation compounds 1-4) are formed due to the heat treatment. (B) Inset showing SAM concentration (nmol/g FW) in tomato extracts during heat treatment of several hours at 75 • C. Error bars indicate the standard deviation based on two repetitions.Reprinted from[69], copyright (2010) with permission from John Wiley and Sons.…”

mentioning

confidence: 98%

Capillary electrophoresis determination of non-protein amino acids as quality markers in foods

Pérez-Míguez

Marina

Castro-Puyana

2016

Journal of Chromatography A

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Determination of S‐Adenosyl‐l‐methionine in Fruits by Capillary Electrophoresis

Abstract: Fast measurements of SAM in fruits and juices are favoured by capillary electrophoresis in a 200 : 50 mm glycine : phosphate (pH 2.5) buffer system.

Cited by 25 publications

References 26 publications

Tissue specific analysis reveals a differential organization and regulation of both ethylene biosynthesis and E8 during climacteric ripening of tomato

Tissue specific analysis reveals a differential organization and regulation of both ethylene biosynthesis and E8 during climacteric ripening of tomato

Targeted Systems Biology Profiling of Tomato Fruit Reveals Coordination of the Yang Cycle and a Distinct Regulation of Ethylene Biosynthesis during Postclimacteric Ripening

Capillary electrophoresis determination of non-protein amino acids as quality markers in foods

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