Determination of IgG Subclasses and Avidity of Antithyroid Peroxidase Antibodies in Patients with Subclinical Hypothyroidism – A Comparison with Patients with Overt Hypothyroidism

Silva, L.M.; Chávez, Juliana Helena; Canalli, Maria Heloisa Büsi da Silva; Zanetti, Carlos Roberto

doi:10.1159/000069069

Cited by 34 publications

(29 citation statements)

References 61 publications

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“…These previous studies might explain the dominance of IgG1 regardless of thyroid functional status. In our study, there were no significant differences in the prevalence and positive percentage of TPOAb IgG1 subclass among the three groups, in agreement with the study by Silva et al [10]. It is well known that HT is a chronic inflammation of the thyroid gland, associated with follicular destruction [22].…”

Section: Discussionsupporting

confidence: 92%

Distribution of immunoglobulin G subclasses of anti-thyroid peroxidase antibody in sera from patients with Hashimoto's thyroiditis with different thyroid functional status

Xie

Gao

et al. 2008

Clinical and Experimental Immunology

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SummaryThe mechanism of disease progression in Hashimoto's thyroiditis (HT) is still unclear. Anti-thyroid peroxidase antibody (TPOAb), a diagnostic hallmark of HT, is principally of the immunoglobulin G (IgG) isotype, and it appears to be a response to thyroid injury. The aim of our study was to evaluate the distribution of IgG subclasses of TPOAb in sera from patients with HT with different thyroid functional status. Sera from 168 patients with newly diagnosed HT were collected and divided into three groups according to thyroid function: patients with hypothyroidism (n = 66), subclinical hypothyroidism (n = 60) and euthyroidism (n = 42). Antigen-specific enzyme-linked immunosorbent assay was used to detect the distribution of TPOAb IgG subclasses. The prevalence of TPOAb IgG subclasses in all patients' sera with HT was IgG1 70·2%, IgG2 35·1%, IgG3 19·6% and IgG4 66·1% respectively. The prevalence of IgG2 in sera from patients with hypothyroidism (51·5%) was significantly higher than that of subclinical hypothyroidism (33·3%) (P < 0·05), and the latter was also significantly higher than that of euthyroidism (11·9%) (P < 0·05). The positive percentage of IgG2 subclass in sera from patients with hypothyroidism and subclinical hypothyroidism was significantly higher than that of euthyroidism (P < 0·05), the prevalence and positive percentage of IgG4 subclass in sera from patients with hypothyroidism and subclinical hypothyroidism was significantly higher than that of euthyroidism respectively (P < 0·05). The predominant TPOAb IgG subclasses in sera from patients with HT were IgG1 and IgG4. Patients with high levels of TPOAb IgG2, IgG4 subclasses might be at high risk of developing overt hypothyroidism.

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Section: Discussionsupporting

confidence: 92%

Distribution of immunoglobulin G subclasses of anti-thyroid peroxidase antibody in sera from patients with Hashimoto's thyroiditis with different thyroid functional status

Xie

Gao

et al. 2008

Clinical and Experimental Immunology

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“…As already mentioned, other authors have similarly observed that autoantibodies to islet autoantigens, such as glutamic acid decarboxylase (GAD65Ab) and tyrosine phosphatase IA-2 (IA-2Ab) (16)(17)(18)26), and to thyroid peroxidase (TPOAb) (27,28), are predominantly of the IgG1 isotype, with minor contribution of the other IgG subclasses. More specifically, a Japanese study reported a different expression of IgG subclass TPOAb in patients with chronic thyroiditis (whose sera were predominantly positive for IgG1-TPOAb), as compared with healthy subjects (whose sera were exclusively associated with the IgG4 isotype) (27).…”

Section: Discussionmentioning

confidence: 69%

Autoantibody responses in autoimmune ovarian insufficiency and in Addison's disease are IgG1 dominated and suggest a predominant, but not exclusive, Th1 type of response

Brozzetti¹,

Marzotti²,

Torre³

et al. 2010

European Journal of Endocrinology

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Objective: Steroid-producing cell autoantibodies (SCAs) directed against 21-hydroxylase autoantibodies (21OHAbs), 17a-hydroxylase autoantibodies (17OHAb), and cholesterol side-chain cleavage enzyme (side-chain cleavage autoantibodies, P450sccAb) characterize autoimmune primary ovarian insufficiency (SCA-POI). The aim of the study was to analyze IgG subclass specificity of autoantibodies related to adrenal and ovarian autoimmunity. Design: We studied 29 women with SCA-POI, 30 women with autoimmune Addison's disease (AAD) without POI, and 14 patients with autoimmune polyendocrine syndrome type 1 (APS1). 21OHAb isotypes were also analyzed in 14 subjects with preclinical AAD. Samples from 30 healthy women served as control group to determine the upper level of normality in the isotype assays. Methods: Immunoradiometric assays with IgG subclass-specific secondary antibodies. Results: In 21OHAb-positive sera, IgG1 isotype was detected in 90% SCA-POI and non-POI AAD sera and 67% APS1 patients. IgG1 isotype was found in 69% 17OHAb-positive SCA-POI and 100% 17OHAb-positive APS1 sera, and in 60% P450sccAb-positive SCA-POI and 80% P450sccAb-positive APS1 sera. For 21OHAb, IgG4 isotype was detected in 17% SCA-POI, 7% non-POI AAD, and 8% APS1 sera. None of the 17OHAb-positive sera was positive for IgG4. In P450sccAb-positive sera, 15% POI and 20% APS1 sera were positive for IgG4. Two 21OHAb-positive SCA-POI (7%), one 21OHAb-positive AAD (3%), three P450sccAb-positive SCA-POI (15%), and two P450sccAb-positive APS1 (20%) sera were positive for IgG4, in the absence of IgG1. All preclinical AAD sera resulted as positive for IgG1-21OHAb, but not for IgG4-21OHAb. Conclusions: The autoantibody responses in POI and AAD are IgG1 dominated, which suggests a predominant Th1 response. Selective IgG4 isotype specificity identified a small subset of patients with Th2-oriented response.

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“…The distribution of IgG subclasses of TPOAb/TGAb might provide insight into the mechanisms of TD in patients with HCV infection (15).…”

Section: Introductionmentioning

confidence: 99%

The presence of thyroid peroxidase antibody of IgG2 subclass is a risk factor for thyroid dysfunction in chronic hepatitis C patients

Shao

Huo

Zhao

et al. 2013

European Journal of Endocrinology

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Objective: To investigate the prevalence of thyroid dysfunction (TD) and IgG subclasses of thyroid autoantibodies (TAs) and to determine the predictive factors of TD in chronic hepatitis C (CHC) patients. Design: Three hundred and twelve untreated hepatitis C virus-infected patients without a history of TD or treatment with thyroid hormones were enrolled in a cross-sectional study. Clinical and biological factors were statistically analyzed to determine the correlation between TD and this patient population. Results: The incidence of TD was 12.5% in CHC patients. Clinical hypothyroidism (5.8%) and subclinical hypothyroidism (3.8%) were more frequent than clinical hyperthyroidism (1.6%) and subclinical hyperthyroidism (1.3%). The percentage of TA-positive patients was significantly higher in people O60 years than in those %60 years (31.9 vs 18.6%; PZ0.042). Positive thyroid peroxidase antibody (TPOAb) was more frequent, and alanine aminotransferase (ALT) levels were lower in patients who displayed TD (TPOAb: 62.1 vs 10.8%, PZ0.000; ALT: 43.5 vs 51 IU/l, PZ0.046). The positive percentage of TPOAb IgG2 subclass in the TD group was significantly higher than that of patients without TD (66.7 vs 16.7%, PZ0.005). Multiple logistic regression analysis indicated that only TPOAb IgG2 subclass positivity was an independent risk factor for TD in CHC patients (odds ratioZ8; 95% CI: 1.225-52.246; PZ0.030). Conclusions: TPOAb IgG2 subclass positivity is a risk factor for TD in CHC patients before antiviral treatment. IgG2 subclass of TPOAb might play an important role in the presence of TD in CHC patients.

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Determination of IgG Subclasses and Avidity of Antithyroid Peroxidase Antibodies in Patients with Subclinical Hypothyroidism – A Comparison with Patients with Overt Hypothyroidism

Cited by 34 publications

References 61 publications

Distribution of immunoglobulin G subclasses of anti-thyroid peroxidase antibody in sera from patients with Hashimoto's thyroiditis with different thyroid functional status

Distribution of immunoglobulin G subclasses of anti-thyroid peroxidase antibody in sera from patients with Hashimoto's thyroiditis with different thyroid functional status

Autoantibody responses in autoimmune ovarian insufficiency and in Addison's disease are IgG1 dominated and suggest a predominant, but not exclusive, Th1 type of response

The presence of thyroid peroxidase antibody of IgG2 subclass is a risk factor for thyroid dysfunction in chronic hepatitis C patients

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