Determination of inosine 5′‐monophosphate and guanosine 5′‐monophosphate in pig feed by capillary zone electrophoresis

Abstract

SummaryA capillary zone electrophoresis method was developed for the determination of IMP and GMP, commonly used as flavor enhancers in poultry feed, in a real sample of complex composition. A baseline separation of inosine 5'-monophosphate and guanosine 5'-monophosphate was achieved within 10 min and the other components in the sample did not interfere with the separation. Quantitative results obtained from pig feed samples are presented. The separation conditions and experimental reproducibility are also discussed.

Analysis of plasma nucleotides in rat by micellar electrokinetic capillary chromatography/electrospray ionization mass spectrometry

“…Originally developed to separate neutral compounds by CE, MEKC has also been found to be useful in the separation of charged compounds like nucleotides . While SDS is the most commonly used surfactant in MEKC separations, dodecyltrimethylammonium bromide (DTAB) and β‐cyclodextrin (β‐CD) have also been used . We selected the cationic surfactants β‐CD and DTAB instead of the anionic surfactant SDS because SDS is generally incompatible with MS instrumentation .…”

“…Reports of separations in acidic electrolytes have also been published, but this approach generally requires longer analytical times. Therefore, some researchers have begun using micellar electrokinetic capillary chromatography (MEKC) to separate nucleotides …”

Electrochemical oxidation of inosine 5′-monophosphate in neutral aqueous solution

“…The molar mass of 414 and 502 corresponds to disilylated N-N bridged dimer (16) and trisilylated C-O-N linked dimer (21) with the loss of both the ribosylphosphate moieties. The formation of tetrasilylated D-ribose (11) having m/z 438 clearly, suggests the hydrolysis of ribosylphosphate groups from the electrolyzed products. The high mass of the molecular ion peak having m/z 970 corresponding to the GC peak at retention time/min 15.72, indicates it to be a trimer or tetramer.…”

“…5 0 -IMP has been reported as specific feeding stimulants for fish meal and striped bass [10]. Besides this, 5 0 -IMP has been used as flavor enhancers in poultry feed [11]. 5 0 -IMP induces a delayed paralysis effect in termites [12] and is reported to exhibit an inhibitory effect on the multiplication of the parasite Babesia gibsonic in infected young erythrocytes and simultaneously retard the maturation of reticulocytes [13].…”

Electrochemical Behaviors and Sensitive Determination of Guanosine-5’-monophosphate on Ionic Liquid Modified Carbon Paste Electrode

“…Therefore, it is necessary to establish a sensitive and reliable method for GMP determination. Up to now different kinds of analytical methods such as as luminescence method, 9 high performance liquid chromatography (HPLC), 10 ion-pair HPLC, 11 capillary zone electrophoresis 12 and electrochemical methods 13,14 had been developed for the sensitive determination of GMP. Some of these methods require expensive instruments with time consuming.…”