Determination of investigation of the link between human and animal Brucella isolates in Iran using multiple-locus variable number tandem repeat method comprising 16 loci (MLVA-16)

Mirkalantari, Shiva; Masjedian, Faramarz; Fateme, Amraee

doi:10.1016/j.bjid.2020.11.008

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…In the present study, B. abortus was rarely detected in sheep and goat samples, similar to other studies (1,6,12,15). This result is due to keeping different livestock spp.…”

Section: Discussionsupporting

confidence: 91%

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Molecular Investigation of Brucella Species Belongs to Sheep and Goats in Seropositive Samples from an Endemic Area of Hamedan Province; Famenin Brucellosis Cohort Study

2023

ARI

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Brucellosis is a zoonotic infectious disease in western regions of Iran, especially in Hamedan province. Following the Famenin brucellosis cohort study, the main aim of the current study was the molecular detection of Brucella species (spp.) in sheep and goats from Famenin, Hamedan, Iran. A total of 23 Brucella-seropositive samples (sheep=21 and goats=2), which had been screened from 1,660 animals in the Famenin cohort study, were used to detect Brucella-DNA using the BCSP31 target gene and IS711 locus. In total, 20 of 23 samples were positive for Brucella infection by using specific primers. Additionally, Brucella melitensis (B. melitensis) and Brucella abortus (B. abortus) were confirmed in 90% (n=18) and 10% (n=2) of positive samples, respectively. There was no sample with the co-infection of B. abortus and B. melitensis. In this study, B. abortus was isolated from one of the goat samples. This is the first report on Brucella spp. in animals in the region. It was found that B. melitensis is the dominant spp. responsible for brucellosis in animals from Famenin. Molecular techniques are reliable tools to detect Brucella infection, especially in cases without serology findings and conclusive results.

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“…In the present study, B. abortus was rarely detected in sheep and goat samples, similar to other studies (1,6,12,15). This result is due to keeping different livestock spp.…”

Section: Discussionsupporting

confidence: 91%

“…Regarding previous reports in Iran, there is no updated data on Brucella spp. in animals from Hamedan province (4,12,15). Following the Famenin brucellosis cohort study, the current project sought to detect Brucella spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular Investigation of Brucella Species Belongs to Sheep and Goats in Seropositive Samples from an Endemic Area of Hamedan Province; Famenin Brucellosis Cohort Study

2023

ARI

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show abstract

“…[ 61 ]. In another study in Iran, three isolates of B. abortus and 51 isolates of B. melitensis were analyzed through the MLVA16 (multiple-locus variable number tandem repeat analysis based on 16 markers) and represented genotypes 46 and genotypes 22 for 80% of isolates [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Whole-genome sequencing for genetic diversity analysis of Iranian Brucella spp. isolated from humans and livestock

et al. 2023

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Molecular epidemiology of brucellosis in Asia: insights from genotyping analyses

Ali,

Mushtaq,

Hassan

et al. 2024

Vet Res Commun

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Determination of investigation of the link between human and animal Brucella isolates in Iran using multiple-locus variable number tandem repeat method comprising 16 loci (MLVA-16)

Cited by 7 publications

References 22 publications

Molecular Investigation of Brucella Species Belongs to Sheep and Goats in Seropositive Samples from an Endemic Area of Hamedan Province; Famenin Brucellosis Cohort Study

Molecular Investigation of Brucella Species Belongs to Sheep and Goats in Seropositive Samples from an Endemic Area of Hamedan Province; Famenin Brucellosis Cohort Study

Whole-genome sequencing for genetic diversity analysis of Iranian Brucella spp. isolated from humans and livestock

Molecular epidemiology of brucellosis in Asia: insights from genotyping analyses

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