Determination of iohexol in human serum by a semi-automated liquid chromatography tandem mass spectrometry method

Vicente, Faye B.; Vespa, Gina; Carrara, Fabiola; Gaspari, Flavio; Haymond, Shannon

doi:10.1016/j.clinbiochem.2015.03.017

Cited by 12 publications

(8 citation statements)

References 16 publications

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“…In 20 paediatric patients, they compared iohexol concentrations (range 15–700 µg/mL) measured by LC-MS/MS in the USA with those determined on the same samples by HPLC-UV by a European reference laboratory in Italy. The concordance between the two methods was very high both for iohexol concentrations and GFR results (difference of <10% between the two laboratories, except for one patient) [ 83 ]. Further research is still required regarding the effect of the measurement method (HPLC or LC-MS/MS) on iohexol (or iothalamate) clearance results.…”

Section: Iohexol: Analytical and Other Metrics Considerationsmentioning

confidence: 99%

Iohexol plasma clearance for measuring glomerular filtration rate in clinical practice and research: a review. Part 1: How to measure glomerular filtration rate with iohexol?

et al. 2016

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While there is general agreement on the necessity to measure glomerular filtration rate (GFR) in many clinical situations, there is less agreement on the best method to achieve this purpose. As the gold standard method for GFR determination, urinary (or renal) clearance of inulin, fades into the background due to inconvenience and high cost, a diversity of filtration markers and protocols compete to replace it. In this review, we suggest that iohexol, a non-ionic contrast agent, is most suited to replace inulin as the marker of choice for GFR determination. Iohexol comes very close to fulfilling all requirements for an ideal GFR marker in terms of low extra-renal excretion, low protein binding and in being neither secreted nor reabsorbed by the kidney. In addition, iohexol is virtually non-toxic and carries a low cost. As iohexol is stable in plasma, administration and sample analysis can be separated in both space and time, allowing access to GFR determination across different settings. An external proficiency programme operated by Equalis AB, Sweden, exists for iohexol, facilitating interlaboratory comparison of results. Plasma clearance measurement is the protocol of choice as it combines a reliable GFR determination with convenience for the patient. Single-sample protocols dominate, but multiple-sample protocols may be more accurate in specific situations. In low GFRs one or more late samples should be included to improve accuracy. In patients with large oedema or ascites, urinary clearance protocols should be employed. In conclusion, plasma clearance of iohexol may well be the best candidate for a common GFR determination method.

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Section: Iohexol: Analytical and Other Metrics Considerationsmentioning

confidence: 99%

Iohexol plasma clearance for measuring glomerular filtration rate in clinical practice and research: a review. Part 1: How to measure glomerular filtration rate with iohexol?

et al. 2016

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“…Three types of monoliths, SMA‐EDMA, PEDAS‐EDMA, and MAA‐EDMA were synthesized and preliminarily investigated. Although successful separation of iohexol on reversed‐phase particle based columns has been reported , no retention of iohexol on the reversed‐phased SMA‐EDMA monolith containing C 18 functional groups was obtained. Similar result was observed from a mixed mode monolith containing both polar –OH and nonpolar –C 17 functional groups; PEDAS‐EDMA monolith.…”

Section: Resultsmentioning

confidence: 99%

“…Reliable and accurate method is needed to control dose amount of iohexol and to determine iohexol clearance for evaluating renal function and diagnosing chronic kidney diseases (CKD) or evaluating risks of developing CKD. For analysis of iohexol in serums, several analytical methods have been developed including HPLC-UV [2,[5][6][7][8][9][10][11][12][13][14], LC-MS/MS [3,4,[15][16][17][18][19], LC-ICP-MS [20], CE [1,[21][22][23], inductively coupled plasma atomic emission spectroscopy (ICP-AES) [24] and X-ray fluorescence spectroscopy (XRF) [25,26]. LC was the most commonly used method for analysis of iohexol [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]; two chromatographic peaks of iohexol stereoisomers could be observed from common RP C 18 columns [2,…”

Section: Introductionmentioning

confidence: 99%

“…In serum, protein is a major part of the sample and can interfere with the analysis or causes clogging of chromatographic column. Several agents have been tested for protein precipitation in serum such as methanol [1,19], TFA [2,8,10], perchloric acid [5,9], zinc sulfate [16,18], and acetonitrile [22,23].…”

Section: Introductionmentioning

confidence: 99%

“…Separation time varied from 1 to 45 min depending on selectivity of the detector used and application. The selective LC–MS/MS method provided fast separation in 1 to 3 min ; however the technique requires expensive instrumentation, special maintenance and operational skills. Sample preparation is another important key to be considered for analytical processes especially for complex samples such as serum, plasma, and urine.…”

Section: Introductionmentioning

confidence: 99%

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Simple and fast analysis of iohexol in human serums using micro‐hydrophilic interaction liquid chromatography with monolithic column

Chaloemsuwiwattanakan

Sangcakul

Kitiyakara

et al. 2016

J of Separation Science

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A simple and rapid method based on micro-liquid chromatography using a synthetic monolithic capillary column was developed for determination of iohexol in human serums, a marker to evaluate the glomerular filtration rate. A hydrophilic methacrylic acid-ethylene dimethacrylate monolith provided excellent selectivity and efficiency for iohexol with separation time of 3 min using a mobile phase of 40:60 v/v 50 mM phosphate buffer pH 5/methanol. Four serum protein removal, methods using perchloric acid, 50% acetonitrile, 0.1 M zinc sulfate, and centrifuge membrane filter were examined. The method of zinc sulfate was chosen due to its simplicity, compatibility with the mobile phase system, nontoxicity, and low cost. Interday calibration curves were conducted over iohexol concentrations range of 2-500 mg/L (R(2) = 0.9997 ± 0.0001) with detection limit of 0.44 mg/L. Intra- and interday precisions for peak area and retention time were less than 2.8 and 1.4%, respectively. The method was successfully applied to serum samples with percent recoveries from 102 to 104. The method was applied to monitor released iohexol from healthy subject. Compared with the commercially available reversed-phase high-performance liquid chromatography method, the presented method provided simpler chromatogram, faster separation with higher separation efficiency and much lower sample and solvent consumption.

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Assessment of Iohexol Serum Clearance by LC-MS/MS with Isotopically Labeled Internal Standard

Jin,

Bertholf,

2023

Methods in Molecular Biology

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Determination of iohexol in human serum by a semi-automated liquid chromatography tandem mass spectrometry method

Cited by 12 publications

References 16 publications

Iohexol plasma clearance for measuring glomerular filtration rate in clinical practice and research: a review. Part 1: How to measure glomerular filtration rate with iohexol?

Iohexol plasma clearance for measuring glomerular filtration rate in clinical practice and research: a review. Part 1: How to measure glomerular filtration rate with iohexol?

Simple and fast analysis of iohexol in human serums using micro‐hydrophilic interaction liquid chromatography with monolithic column

Assessment of Iohexol Serum Clearance by LC-MS/MS with Isotopically Labeled Internal Standard

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