Simple and fast analysis of iohexol in human serums using micro‐hydrophilic interaction liquid chromatography with monolithic column

Abstract

A simple and rapid method based on micro-liquid chromatography using a synthetic monolithic capillary column was developed for determination of iohexol in human serums, a marker to evaluate the glomerular filtration rate. A hydrophilic methacrylic acid-ethylene dimethacrylate monolith provided excellent selectivity and efficiency for iohexol with separation time of 3 min using a mobile phase of 40:60 v/v 50 mM phosphate buffer pH 5/methanol. Four serum protein removal, methods using perchloric acid, 50% acetonitrile, 0.1 M zinc sulfate, and centrifuge membrane filter were examined. The method of zinc sulfate was chosen due to its simplicity, compatibility with the mobile phase system, nontoxicity, and low cost. Interday calibration curves were conducted over iohexol concentrations range of 2-500 mg/L (R(2) = 0.9997 ± 0.0001) with detection limit of 0.44 mg/L. Intra- and interday precisions for peak area and retention time were less than 2.8 and 1.4%, respectively. The method was successfully applied to serum samples with percent recoveries from 102 to 104. The method was applied to monitor released iohexol from healthy subject. Compared with the commercially available reversed-phase high-performance liquid chromatography method, the presented method provided simpler chromatogram, faster separation with higher separation efficiency and much lower sample and solvent consumption.

Functionalized monolithic columns: Recent advancements and their applications for high-efficiency separation and enrichment in food and medicine

“…(Lee et al, 2012) used online column conversion ultrafast HPLC-MS-/MS to obtain a good Frontiers in Chemistry frontiersin.org separation of eight barbiturates in human plasma, within 3 min, with a C18 monolithic column, and achieved a quantitative accuracy of 92.0-108%, which is useful in the clinical and toxicological analysis of barbiturates. Chaloemsuwiwattanakan et al (2016) developed a micro-LC-based synthetic capillary monolithic column for the rapid detection of iodohexanol in human serum, with a detection limit of 0.44 mg L −1 , and a recovery rate of 102-104%. Effective dose control of iodohexanol and assessment of renal function were reported (Denis et al, 2008;De Baere et al, 2012;Hellqvist et al, 2015).…”