Determination of Iridoid Glucosides for Quality Assessment of Herba Oldenlandiae by High-Performance Liquid Chromatography

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“…Previous phytochemical studies of OD have demonstrated that Herba oldenlandiae contains a majority of iridoid glucosides, triterpenoids, flavonoids, and polysaccharides (Liang et al, 2006). In the present study, we have isolated and identified from the most effective chloroformic extract of OD two bioactive compounds able to selectively affect breast cancer cells growth.…”

Apoptotic effect of Oldenlandia diffusa on the leukaemic cell line HL60 and human lymphocytes

“…This absence is likely to be due to ursolic acid being insoluble in water and thus not being present in the aqueous extracts of Oldenlandia diffusa used in this study. More recent investigations have found that the main components of Oldenlandia diffusa are iridoid glucosides (Kim et al, 2005;Liang et al, 2006). These types of compounds have been found to elicit a range of biological activities, including antitumour activity (Dinda et al, 2007).…”

Oleanolic acid isolated from Oldenlandia diffusa exhibits a unique growth inhibitory effect against ras-transformed fibroblasts

“…was purchased from an herbal market in Luoding County, Guangdong Province, China in 2005. The authentication was performed as described in Liang et al (2006). Quality assessment and chemical profiling of the herb were performed by HPLC (Liang et al 2007).…”

“…Quality assessment and chemical profiling of the herb were performed by HPLC (Liang et al 2007). UA, OA and IG1-3 were isolated and purified from the dried herb of O. diffusa as described (Liang et al 2006). In brief, the pulverized whole plant of O. diffusa (10 g) was extracted with 200 ml ddH 2 O, methanol or 95% ethanol for 1 h and repeated once by the heating reflux method.…”

“…Their structures ( Fig. 1) were determined on the basis of HR-MS, 1 H-NMR and 13 C-NMR spectral data (Liang et al 2006). The purity of these five compounds, as determined by HPLC-UV, was greater than 97%.…”