Determination of ivermectin in cattle and sheep tissues using high-performance liquid chromatography with fluorescence detection

Tway, Patricia C.; Wood, James S.; Downing, George V.

doi:10.1021/jf00107a041

Cited by 100 publications

(63 citation statements)

References 2 publications

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“…The time for 1/2 of the ivermectin to leave cattle liver was 4.9 days compared to 7.1 days to leave reindeer fat at the same dosage rate (Tway et aL, 1981).…”

Section: Discussionmentioning

confidence: 93%

Safety, efficacy, and tissues residues of ivermectin in reindeer

Dieterich

Craigmill

1990

Ran

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Safety, efficacy, and tissue residues of ivermectin, a broad spectrum parasiticide, were determined in Alaskan reindeer (Rangifer tarandus). Reindeer treated at 5 times and 10 times the standard dose of 200 mcg/kg had no detectable physical or behavioral reactions to ivermectin injected subcutaneously in the midcervical area. Ivermectin eliminated essentially 100% of reindeer warble larvae (Hypoderma (Oedemagena) tarandi). Tissue levels of ivermectin in back fat, injection site, muscle, liver, and kidney collected 3, 10, 17, and 24 days post injection were determined. All tissues levels rapidly declined and were approaching low unmeasurable amounts at the end of the 24 day test period. Ivermectin is a safe effective parasiticide that has been used successfully to threat thousands of reindeer in Alaska.

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“…The time for 1/2 of the ivermectin to leave cattle liver was 4.9 days compared to 7.1 days to leave reindeer fat at the same dosage rate (Tway et aL, 1981).…”

Section: Discussionmentioning

confidence: 93%

Safety, efficacy, and tissues residues of ivermectin in reindeer

Dieterich

Craigmill

1990

Ran

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“…At first, very long reaction time necessary to obtain stable derivatives and the process was highly susceptible to the content of even traces of water (19). The change of the catalyst significantly reduced the time of reaction but the method was still long and required SPE purification of the obtained derivatives (20). A further modification of the protocol was achieved by Nagata et al (16), who discovered that simple dilution of the final extract with acetonitrile was sufficient for removing interferences in FLD chromatogram.…”

Section: Discussionmentioning

confidence: 99%

Control of Residues of Five Macrocyclic Lactones in Cow Milk By Liquid Chromatography with Fluorescence Detection

Szprengier-Juszkiewicz

Jedziniak

Olejnik

et al. 2012

Bulletin of the Veterinary Institute in Pulawy

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A sensitive and reliable method has been developed and validated to determine residues of abamectin, doramectin, eprinomectin, ivermectin, and moxidectin in bovine milk. Isolation of the analytes from milk was performed with the use of liquidliquid extraction with acetonitrile in the presence of sodium chloride. The extract was defatted with hexane and cleaned up using solid phase extraction (C8 cartridge) after forming ion pairs with triethylamine. The analytes were derivatized with N, Ndimethylformamide, acetic acid anhydride, and N-methylimidazole (100°C, 90 min). The derivatives were determined by reverse phase liquid chromatography with fluorescence detection (excitation and emission wavelength 365 nm and 475 nm, respectively). Recoveries of the lactones from milk samples fortified at 10-30 µg kg -1 ranged from 52% to 80% with intra-laboratory reproducibility (CV) of 12.7%-22.8%. The critical concentrations (decision limit, CCα and detection capability, CCβ) were in accordance with target limits. The method has been verified in the proficiency studies by EURL/CVL Berlin (all z-scores in the range of ±2). The method was transferred to routine laboratories, verified in inter-laboratory comparison and successfully applied in the National Residue Control Plan.

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“…Avermectin residues in edible tissues and milk are usually determined using liquid chromatography (LC) with fluorescence detection after dehydration of the molecule to form an aromatic fluorescent moiety. [5][6][7][8] While the traditional extraction method is very labor intensive, more recent isolation methods have been developed for these drugs which utilize solid phase extraction techniques. [6][7][8] For regulatory purposes, unambiguous identification of the suspect residues found in the samples by the determinative methods is critical.…”

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confidence: 99%

Confirmation of avermectin residues in food matrices with negative-ion atmospheric pressure chemical ionization liquid chromatography/mass spectrometry

Turnipseed

Roybal

Rupp

et al. 1999

Rapid Commun. Mass Spectrom.

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A multi-residue LC/MS method has been developed to confirm avermectin drug residues in several food matrices. Ivermectin (IVR), doramectin (DOR), eprinomectin (EPR) and moxidectin (MOX) are confirmed using atmospheric pressure chemical ionization (APCI) with negative ion detection and selected ion monitoring of three to four ions for each compound. The drug residues are extracted from tissue or milk using previously published procedures. IVR and DOR are confirmed at 20 ppb levels in fortified salmon muscle; IVR is also confirmed in tissue from salmon dosed with the drug. Residues of DOR, IVR, and EPR are confirmed in fortified milk at the 20 ppb level and in fortified beef liver at 40 ppb. Residues of MOX can also be confirmed in these matrices, but at slightly higher levels (40-80 ppb).

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Determination of ivermectin in cattle and sheep tissues using high-performance liquid chromatography with fluorescence detection

Cited by 100 publications

References 2 publications

Safety, efficacy, and tissues residues of ivermectin in reindeer

Safety, efficacy, and tissues residues of ivermectin in reindeer

Control of Residues of Five Macrocyclic Lactones in Cow Milk By Liquid Chromatography with Fluorescence Detection

Confirmation of avermectin residues in food matrices with negative-ion atmospheric pressure chemical ionization liquid chromatography/mass spectrometry

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