Determination of leaf status of soybean varieties on shading : chlorophyll and chloroplast

Abstract

Abstract. Mawarni L, Nisa TC, Napitupulu JA, Karyudi. 2019. Determination of leaf status of soybean varieties on shading. Biodiversitas 20: 615-620. The shading tolerant soybean varieties could be identified through the character growth such as leaf status. This study was aimed to obtain the shading tolerant of soybean variety based on their seed size and on the leaf status. Design of experiments was a split-plot design with three replications. The level of shading as the main plot with four levels of treatment was without shading, 30%, 50%, and 70% shading. Soybean varieties as the subplot had 4 genotypes namely Anjasmoro, Pangrango, Tanggamus, and Nanti wherein one variety comes with large seed; two come with medium seeds and the other one comes with small seed. The leaf status for determination was the greenness of leaves, the sum of chlorophyll a, chlorophyll b, total chlorophyll a and b, the forms of chloroplasts and leaf area. The soybean varieties with different seed sizes showed the differences only on the leaf area but not on leaf greenness or on the sum of chlorophyll. Nanti variety, having small seed, turns out to have the largest leaf area but is not shade tolerant. The large leaf area may be due to the forming of tetra foliate leaves on Nanti variety. The form of chloroplast of Anjasmoro variety in 70% shading is better than other varieties. Therefore, determining leaf status can identify shading tolerant of soybeans.

Variation in leaf characters and agronomical traits of Yard-long bean genotypes between Dimocarpus longan and Psidium guajava stand

“…This condition will lead to changes in morphology and physiology as a strategy for dealing with stress. Shade conditions caused an increase in chlorophyll-a and chlorophyll-b but reduced the ratio of chlorophyll a/b (Mawarni et al 2019). In addition, Gonçalves et al (2001) added shade to increase the carotenoid content of legume leaves.…”

Agronomic diversity of several soybean putative mutant lines resulting from gamma-rays irradiation in M6 generation

“…Mutation induction with gamma rays is commonly used to obtain genetic diversity in cultivated plants, especially soybeans. Several researchers have previously used gamma-rays on various soybean genotypes, including Sohag, BARI Soybean-5, Bangladesh Soybean-4, and BAU-S/64, for genetic improvement of yield attributes (Malek et al 2014), soybean line VX04-6828 for better agronomic performance and seeds composition (Nobre et al 2019), Anjasari soybean for high seed yield and large seed size (Mawarni et al 2019;Yoel and Rachmadi 2020), Korean cultivars Danbaek and Daepung for highest linolenic acid contents (Hong et al 2019), Kipas Putih soybeans for early maturity and high yields (Nilahayati et al 2016;Nilahayati et al 2019), Denna 2 variety for shadetolerant (Harsanti et al 2020) and induction of genetic diversity of soybean genotypes PGRI-15, KY EXOTIC, NARC-, AJMERI-2, AJMER-I (Hussain et al 2020).…”