Determination of Leptin Adiponectin, LDL/HDL ratio with substantial role of Adiponectin and its Receptor

Abstract

Aim: To determine Leptin/Adiponectin ratio, LDL/HDL ratio and AdipoR1 in obese and healthy subjects along with their respective lipid status. Methods: This cross-sectional comparative study was conducted in Sialkot city. One hundred and thirty-two participants took part in this research. Participants were equally divided into two groups containing non-obese and obese subjects. Mean age was 39.6±0.97 years. Mean BMI for obese subjects was 31.55±0.6 while non-obese group BMI was 20.5±0.2. Individuals with conditions and history of drugs were excluded. Informed and written consent was obtained prior to fasting blood sampling. Serum extraction and proper storage for later testing was carried out. ELISA method used for Adiponectin, AdiopR1 and leptin estimations while lipid profile was determined by Randox Diagnostics kits, using micro lab. SPSS v. 26. was used for comparison between by Mann-Whitney U tes. Results: Higher levels of Leptin/Adiponectin ratio(0.85±0.1) and LDL/HDL ratio (3.39 ± 0.1), serum Adiponectin (545± 73.3 ug/L), leptin (320.7±50.3 pg/mL) and AdipoR1 (28.9± 2.8 ng/mL) in obese when compared with healthy individuals, Leptin/Adiponectin ratio (0.44 ± 0.07 ) and LDL/HDL ratio (2.56 ± 0.08) Adiponectin (834± 70.6 ug/L), AdiopoR1 (17.8± 1.97 ng/mL), leptin (224.4±168.7 pg/mL). Correlation of adiponectin found positive for AdipoR1(r=0.336,p<0.05) and Leptin(r=0263,p<0.05) in obese subjects. L/A ratio correlated positive with leptin (r=0.644,p<0.05) in obese while in healthy subjects (leptin r=0.409,p<0.05,adiponectin r=-0.408,p<0.05 and HDL r=0.266,p<0.05).. Conclusion: The Leptin/Adiponectin ratio was found higher in obese subjects 0.85 as compared to healthy ones 0.44. Also the LDL/HDL ratio was found higher (3.39) when compared to non-obese (2.56), suggesting these ratios as a suitable marker to estimate metabolic disturbances and underlying dyslipidemia in the obese subjects. Key Words: Adiponectin, LDL/HDL ratio, Leptin, Leptin/Adiponectin ratio, Obesity