Determination of lethal dose of Aeromonas hydrophila Ah17 strain in snake head fish Channa striata

Samayanpaulraj, Vignesh; Velu, Vijay; Ramesh, U.

doi:10.1016/j.micpath.2018.11.035

Cited by 44 publications

(31 citation statements)

References 26 publications

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“…Clinical signs of involvement of the respiratory system, hemorrhages in the opercular region, reduced breathing movements and erratic swimming at the surface, with difficulty in remaining at the bottom of the aquarium, were observed in all A. gigas individuals that were inoculated with A. hydrophila plus A. jandaei . These characteristics seem to be common of infection by Aeromonas species because they were also previously reported in other studies (Dias et al., 2016; Kumar et al., 2016; Proietti‐Junior et al., 2017; Samayanpaulraj et al., 2019).…”

Section: Discussionsupporting

confidence: 77%

Experimental co‐infection by Aeromonas hydrophila and Aeromonas jandaei in pirarucu Arapaima gigas (Pisces: Arapaimidae)

Proietti

Lima

Roges

et al. 2021

Aquaculture Research

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In fish, bacteriosis has been widely linked with Aeromonas species, but co‐infection by these bacteria has been little addressed. The aims of this study were to report on an outbreak of disease in pirarucu Arapaima gigas caused by Aeromonas and to investigate experimental co‐infection and characterize resistance profile, virulence factors and phenotypic and molecular differentiation. Fish samples with clinical signs of bacteriosis were collected and used to study experimental co‐infection. The bacterial isolates were characterized phenotypically as Aeromonas hydrophila and Aeromonas jandaei. Virulence genes aerA, gcat, lip, dnase and hlyA were detected using the polymerase chain reaction, while the alt, act and ser genes were not found. Resistance to imipenem and ceftriaxone was observed; however, all isolates were susceptible to most of the antibiotics assayed. Phenotypic tests to determine the presence of metallo‐β‐lactamases showed positivity only for A. jandaei strains. Assays for the resistance genes kpc, ndm, imp, oxa‐48 and vim showed negative results. The co‐infection and pathogenicity of A. hydrophila in association with A. jandaei in A. gigas, established in accordance with Koch's postulate, provided experimental support for the existence of synergism between these bacteria. This has several implications relating to occurrences of this co‐infection and determinants of virulence.

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Section: Discussionsupporting

confidence: 77%

Experimental co‐infection by Aeromonas hydrophila and Aeromonas jandaei in pirarucu Arapaima gigas (Pisces: Arapaimidae)

Proietti

Lima

Roges

et al. 2021

Aquaculture Research

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“…hydrophila Ah17 infection in C. striata, which showed the pathogenic potential of the strain against freshwater fish (Samayanpaulraj, Velu, & Uthandakalaipandiyan, 2019).…”

Section: Discussionmentioning

confidence: 89%

“…In the present study, A. hydrophila Ah17 secretes amylase, lipase, and protease and it may contribute pathogenicity to the host. Ulcerative lesions and depigmentation on the caudal fins were observed during the course of A. hydrophila Ah17 infection in C. striata , which showed the pathogenic potential of the strain against freshwater fish (Samayanpaulraj, Velu, & Uthandakalaipandiyan, ).…”

Section: Discussionmentioning

confidence: 98%

Identification and characterization of virulent Aeromonas hydrophila Ah17 from infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan

Samayanpaulraj

Sivaramapillai

Palani

et al. 2020

Food Science & Nutrition

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Aeromonas hydrophila, an inhabitant in the aquatic ecosystem is considered as an important foodborne bacterial zoonotic pathogen in aquaculture. The present study aimed to identify virulent A. hydrophila from naturally infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan. Rimler Shotts (RS) and blood agar medium identified the presence of pathogenic Aeromonas sp. from the infected C. striata. A. hydrophila Ah17 was identified using 16S rRNA nucleotide sequence. Extracellular enzymes such as amylase, lipase, and protease were screened in A. hydrophila Ah17. Antibiotic susceptibility tests showed A. hydrophila Ah17 was highly resistant against β‐lactam, glycopeptide, macrolides, phosphonic, fucidin, and oxazolidinone classes of antibiotics. Virulent genes such as hemolysin (aer and hly), heat‐labile enterotoxin (act), cytotonic heat‐stable enterotoxin (ast), elastase (ahyB), and lipase (lip) were identified. Growth and the viable cell population of virulent A. hydrophila Ah17 were significantly reduced in a dose‐dependent manner against shrimp chitosan (CHS) from Penaeus indicus (P. indicus). Thus, the present study isolated virulent A. hydrophila Ah17 from the environmental source and characterized in vitro with shrimp chitosan.

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“…load and effects on the host, where an increase in the bacterial load results in magnified deleterious effects at the host level (i.e. morbidity and/or mortality) [ 50 ]. However, our results do not evidence such relationship.…”

Section: Discussionmentioning

confidence: 99%

Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions’ prevalence

et al. 2021

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Assessments regarding health aspects of Iberian leuciscids are limited. There is currently an information gap regarding effects of infectious diseases on these populations and their role as a possible conservation threat. Moreover, differences in susceptibility to particular agents, such as Aeromonas spp., by different species/populations is not clear. To understand potential differences in Aeromonas diversity and load, as well as in the prevalence and proportion of skin lesions, in fishes exposed to similar environmental conditions, an observational study was implemented. Using a set of 12 individuals belonging to two sympatric Iberian leuciscid species (Squalius pyrenaicus and Iberochondrostoma lusitanicum), the skin lesion score in each individual was analyzed. Furthermore, a bacterial collection of Aeromonas spp. isolated from each individual was created and isolates’ load was quantified by plate counting, identified at species level using a multiplex-PCR assay and virulence profiles established using classical phenotypic methods. The similarity relationships of the isolates were evaluated using a RAPD analysis. The skin lesion score was significantly higher in S. pyrenaicus, while the Aeromonas spp. load did not differ between species. When analyzing Aeromonas species diversity between fishes, different patterns were observed. A predominance of A. hydrophila was detected in S. pyrenaicus individuals, while I. lusitanicum individuals displayed a more diverse structure. Similarly, the virulence index of isolates from S. pyrenaicus was higher, mostly due to the isolated Aeromonas species. Genomic typing clustered the isolates mainly by fish species and skin lesion score. Specific Aeromonas clusters were associated with higher virulence indexes. Current results suggest potential differences in susceptibility to Aeromonas spp. at the fish species/individual level, and constitute important knowledge for proper wildlife management through the signalization of at-risk fish populations and hierarchization of conservation measures.

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Determination of lethal dose of Aeromonas hydrophila Ah17 strain in snake head fish Channa striata

Cited by 44 publications

References 26 publications

Experimental co‐infection by Aeromonas hydrophila and Aeromonas jandaei in pirarucu Arapaima gigas (Pisces: Arapaimidae)

Experimental co‐infection by Aeromonas hydrophila and Aeromonas jandaei in pirarucu Arapaima gigas (Pisces: Arapaimidae)

Identification and characterization of virulent Aeromonas hydrophila Ah17 from infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan

Sympatric threatened Iberian leuciscids exhibit differences in Aeromonas diversity and skin lesions’ prevalence

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