Identification and characterization of virulent <i>Aeromonas hydrophila</i> Ah17 from infected <i>Channa striata</i> in river Cauvery and in vitro evaluation of shrimp chitosan

Samayanpaulraj, Vignesh; Sivaramapillai, Muthukumar; Palani, Sankara Naynar; Govindaraj, Krishnaveni; Velu, Vijay; Ramesh, U.

doi:10.1002/fsn3.1416

Cited by 19 publications

(7 citation statements)

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“…Due to the complexity of Aeromonas species identification, the specific genes sequence analyses were conducted after PCR examination [ 26 ]. The DNAs of the PCR-positive isolates were used for sequencing the housekeeping genes gyrB (~1100 pb) and rpoB (560 bp) with the corresponding primer sets presented in Table 5 .…”

Section: Methodsmentioning

confidence: 99%

Prevalence, Virulence Gene Distribution and Alarming the Multidrug Resistance of Aeromonas hydrophila Associated with Disease Outbreaks in Freshwater Aquaculture

Nhinh

Van

et al. 2021

Antibiotics

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The study aims to evaluate the infection prevalence, virulence gene distribution and antimicrobial resistance of Aeromonas hydrophila associated in diseased outbreaks of cultured freshwater fish in Northern Vietnam. The confirmed A. hydrophila were screened for the presence of the five pitutative-virulence genes including aerolysin (aerA), hemolysin (hlyA), cytotonic enterotoxin (act), heat-labile cytotonic enterotoxin (alt), and heat-stable enterotoxin (ast), and examined the susceptibility to 16 antibiotics. A total of 236 A. hydrophila isolates were recovered and confirmed from 506 diseased fish by phenotypic tests, PCR assays, and gyrB, rpoB sequenced analyses, corresponding to the infection prevalence at 46.4%. A total of 88.9% of A. hydrophila isolates harbored at least one of the tested virulence genes. The genes aerA and act were most frequently found (80.5% and 80.1%, respectively) while the ast gene was absent in all isolates. The resistance to oxacillin, amoxicillin and vancomycin exhibited the highest frequencies (>70%), followed by erythromycin, oxytetracycline, florfenicol, and sulfamethoxazole/trimethoprim (9.3–47.2%). The multiple antibiotic resistance (MAR) index ranged between 0.13–0.88 with 74.7% of the isolates having MAR values higher than 0.2. The results present a warning for aquaculture farmers and managers in preventing the spread of A. hydrophila and minimizing antibiotic resistance of this pathogen in fish farming systems.

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Section: Methodsmentioning

confidence: 99%

Prevalence, Virulence Gene Distribution and Alarming the Multidrug Resistance of Aeromonas hydrophila Associated with Disease Outbreaks in Freshwater Aquaculture

Nhinh

Van

et al. 2021

Antibiotics

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“…A quitosana apresenta boa atividade antimicrobiana frente uma grande variedade de microrganismos patogênicos e deteriorantes, principalmente, de alimentos, incluindo fungos e bactérias Gram+ e Gram-(DUTTA; TRIPATHI; DUTTA, 2012). Samayanpaulraj et al (2020) investigaram o potencial antibacteriano da quitosana de camarão (Penaeus indicus) frente a isolados de Aeromonas hydrophila virulenta Ah17 e observaram que ela foi capaz de controlar o crescimento dessa cepa. Nanopartículas de quitosana também já foram reportadas com ação antimicrobiana frente a uma gama de bactérias, fungos, leveduras e algas, através de estudos in vivo quanto in vitro, sendo utilizado como revestimento antimicrobiano, em processos cicatriciais (feridas, queimaduras), na prevenção de doenças infecciosas e na inibição de agentes deteriorantes de alimentos, especialmente, frutas (ROZMANET al., 2019).…”

Section: Aplicações Biotecnológicas Da Quitosanaunclassified

MINI-REVISÃO FOCADA NAS APLICAÇÕES BIOTECNOLÓGICAS DA QUITOSANA: EMBALAGENS, ALIMENTOS & BEBIDAS

Oliveira¹,

Cunha²,

Batista³

et al. 2020

Ciência, Tecnologia E Inovação: Do Campo À Mesa

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Chitosan is a polysaccharide obtained from chitin and agricultural, therapeutic, and industrial processes. Among these, the food industry seems to be the one that exploits this raw material preparation, or even as beverage additives biotechnological applications of chitosan in the food industry. For that, searches in scientific bases were used to carry out the bibliographic survey (ScienceD Springer, TandFonline, PubMed, Wiley Online Library and Google Scholar). A large volume of publications was identified, signaling the importance of the topic for the industrial area, from the available publications properties (biodegradability, biocompatibility, atoxicity) and that occasional deficiencies can be addressed by associating this polymer to other natural compounds. The scientific volume

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“…Gram-negative, rod-shaped bacteria that are facultative anaerobes, non-spore-forming, and belong to the genus Aeromonads are widely distributed in aquatic environments [1] . Aeromonads can be classified into two categories: psychrophiles, exemplified by Aeromonas salmonicida , and mesophiles, represented by Aeromonas hydrophila , which can infect both warm-blooded and cold-blooded organisms [2] . Sewage, sewage pollutants, surface water, groundwater, and sewage-contaminated waterways have all been shown to contain the Aeromona s species [3] .…”

Section: Introductionmentioning

confidence: 99%

Detection of Aeromonas hydrophila possessing aerolysin gene using gold nanoparticle probe

Guia,

Monserate

et al. 2024

J Adv Vet Anim Res

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Objective: The aerolysin (aerA) is a virulence indicator used to identify the pathogenicity of the Aeromonas strain. Targeting a pathogen’s crucial virulence gene for detection is essential, as it determines the potential threat to the host. This study aimed to develop a gold nanoparticle (AuNP) probe for detecting the gene aerA in Aeromonas hydrophila among field samples. Materials and Methods: Kidney samples among both healthy and sick Nile tilapias in five provinces of Luzon Island were collected for bacterial analysis. Screening using specific primers targeting aerA was conducted in parallel with testing the AuNPs probe on the same sample set. The positive control provided by BFAR-NFLD, confirmed by polymerase chain reaction (PCR) assay, was used as a positive sample containing the target gene. Results: The AuNP probe demonstrated a computed accuracy of 81.32%, sensitivity of 100%, and specificity of 81.26%. Among the 257 reactions, 59 were false positives, while no false negative results were observed. The AuNP probe could detect aerA at levels as low as 30 ng/μl. The low prevalence of the target gene may be attributed to the use of general media instead of specific media like Rimler-Shotts agar. Conclusion: The established colorimetric detection method for A. hydrophila with the aerA gene offers a swift alternative to PCR, negating the requirement for advanced equipment like a thermal cycler.

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Identification and characterization of virulent Aeromonas hydrophila Ah17 from infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan

Cited by 19 publications

References 50 publications

Prevalence, Virulence Gene Distribution and Alarming the Multidrug Resistance of Aeromonas hydrophila Associated with Disease Outbreaks in Freshwater Aquaculture

Prevalence, Virulence Gene Distribution and Alarming the Multidrug Resistance of Aeromonas hydrophila Associated with Disease Outbreaks in Freshwater Aquaculture

MINI-REVISÃO FOCADA NAS APLICAÇÕES BIOTECNOLÓGICAS DA QUITOSANA: EMBALAGENS, ALIMENTOS & BEBIDAS

Detection of Aeromonas hydrophila possessing aerolysin gene using gold nanoparticle probe

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