Determination of lipid content of oleaginous microalgal biomass by NMR spectroscopic and GC–MS techniques

Sarpal, A. S.; Teixeira, Cláudia Maria Luz Lapa; Silva, Paulo R. M.; Lima, G.; Silva, Samantha R.; Monteiro, Thays Vieira da Costa; Cunha, Valnei Smarçaro da; Daroda, Romeu J.

doi:10.1007/s00216-015-8613-6

Cited by 34 publications

(88 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Practically, algae can yield several times larger quantities of oil from lipids (40 -120 ton/ha year) compared to regular oil crops such as soybean and palm oils (0.2 -6 ton/ ha year) [1][2][3][4][5][6] . Oleaginous microbial oil is produced by the cultivation of algae and bacteria, which is promising sustainable sources of biofuels for the future [5][6][7][8] . Microalgae are sunlight-driven cell factories that convert carbon dioxide to biofuels.…”

Section: Introductionmentioning

confidence: 99%

“…They can provide several different types of renewable biofuels such as biodiesel, methane produced by anaerobic digestion of the algae biomass and photo biologically produced biohydrogen [1] and green hydrocarbons biofuel made by catalytic deoxygenation [2] . Many microalgae such as Chlorella, Baruni, Scenedesmus, Tetraselmis etc., have the ability to produce substantial amounts of 20 -50% of dry cell weight of TAG as a storage lipids on account of photo-syntheses [3][4][5][6][7][8] . Algae biomass is comprised of neutral lipids (TAG; mono, di, tri glycerides; FFA, free fatty acids), polar lipids (glyco/glycerophospho), proteins, alcohols, carbohydrates, vitamins, enzymes etc.…”

Section: Introductionmentioning

confidence: 99%

“…Algae biomass is comprised of neutral lipids (TAG; mono, di, tri glycerides; FFA, free fatty acids), polar lipids (glyco/glycerophospho), proteins, alcohols, carbohydrates, vitamins, enzymes etc. The neutral lipids are important components of interest to energy sector because of their high potential to produce biodiesel [8] . Basically, the fatty acid composition of fat or glycerides has the same profile resembling edible/non-edible/fish oil (C12 to C20/24), therefore, they can be considered as potential substitutes for the fossil oils [2,[7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

“…The neutral lipids are important components of interest to energy sector because of their high potential to produce biodiesel [8] . Basically, the fatty acid composition of fat or glycerides has the same profile resembling edible/non-edible/fish oil (C12 to C20/24), therefore, they can be considered as potential substitutes for the fossil oils [2,[7][8][9] . TAG from algae biomass is converted into fatty acid methyl ester (FAME) (biodiesel), usually by chemical hydrolysis or in vivo enzymatic transesterification [10,11] .…”

Section: Introductionmentioning

confidence: 99%

“…Besides these complex polar components, hydrocarbons similar in nature to crude oil fractions are found to be present in certain strains of microalgae such as green colonial microalgae Botryococcus brauni [7,12,13] . The composition and nature of these components including fatty acids are dependent upon the types of species/strains, culture media and feed used for their cultivation [1][2][3][4][5][6][7][8] . Stressed culture conditions such as nitrogen limitation or nutrient imbalance as well as variation in temperature, pH, light, carbon source and salinity, can be applied during cultivation period for stimulating increase in biomass productivity for high biodiesel potential [14] .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

comparison of oil extraction methods for algae by NMR and Chromatographic techniques

Sarpal¹,

Sharma²,

Scott³

et al. 2016

JABST

View full text Add to dashboard Cite

The present study focuses on identification and compositional characterization of neutral (tri acyl glycerides, TAG; free fatty acids, FFA) and polar (glyco/ glycerophospho) lipids, and fatty acid chains constituting these lipids in the cyclohexane, dichloromethane and methanol extracts or algal oils of microalgae biomasses. Algal oils were generated by step wise Accelerated Solvent and Soxhlet extraction methods from six microalgae biomasses (Scenedesmus dimorph, Chlorella vulgaris, Spirulina, SD Flakes, Cyanothece and Chlamydomonas) obtained from commercial sources. The analytical protocol developed based on NMR (300 & 400 MHz)( 1 H & 13 C), IR, GPC, GC-FID and GC-MS techniques has provided authentic quantitative information about the nature of neutral lipids, polar lipids, fatty acid profiles, oxylipins (oxo fatty acids) and hydrocarbons present in the extracts. The analyses of fractions generated by silica column chromatographic separation of solvent extracts have facilitated demarcation of 1 H NMR chemical shift regions of functional groups corresponding to neutral and polar lipids. Specifically, α (n-3) and γ (n-6) C18:3 were differentiated in the Spirulina by critical examination of 1 H NMR spectral features of algal oils from various biomasses. The comparative results by different analytical techniques have shown that newly developed methods based on 1 H NMR techniques can provide direct and rapid quantitative results of neutral lipids components including poly unsaturated fatty acid/ester (PUFA/PUFE) in the algal oils without prior sample treatment. The neutral lipids results obtained by NMR and GPC show good agreement as compared by linear regression analyses. The results showed that nature and contents of neutral lipids, and fatty acids profile in the extracts were dependent upon the methods of extraction and the polarity index of solvent used for extraction, and specific to types of algae strains. The application of NMR and IR techniques enabled assessment of the solvent extraction efficiency towards extraction of neutral and polar lipids including types of fatty acids constituting these lipids.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

comparison of oil extraction methods for algae by NMR and Chromatographic techniques

Sarpal¹,

Sharma²,

Scott³

et al. 2016

JABST

View full text Add to dashboard Cite

show abstract

Lipid quantification techniques for screening oleaginous species of microalgae for biofuel production

Hounslow

Noirel

Gilmour

et al. 2016

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

This review examines the available literature on quantifying lipids in microalgae suitable for biofuels research. It discusses their advantages and disadvantages, their prevalence in the literature, and draws conclusions about the best way to approach choosing a suitable lipid measurement technique for microalgal biofuels research. We conclude that the method must be chosen based on the following key criteria: (1) the level of detail required in the results, and (2) the amount of biomass that can be spared for the assay. This review establishes that no method can be used as a "golden standard" for all microalgae. However, we present a systematic decision chart to choose the best measurement method or combination of methods to provide a guide to those wishing to understand the differences between the myriad of lipid measurement techniques.Practical applications: This review will allow researchers new to the field to choose the most appropriate techniques for quantifying lipids in the microalgal species under study. The review is also intended to act as a gateway to the wider literature and will enable researchers to look in depth at a particular technique before carrying out experimental work.

show abstract

Mixotrophic cultivation of Chlorella vulgaris in sugarcane molasses preceding nitrogen starvation: Biomass productivity, lipid content, and fatty acid analyses

Laraib

Manzoor

Javid

et al. 2021

Env Prog and Sustain Energy

View full text Add to dashboard Cite

In the present study, a precharacterized oleaginous microalgal species (Chlorella vulgaris) was exploited for its biomass productivity, lipid yield, and fatty acid analysis under mixotrophic and photoautotrophic conditions. For the purpose, the microalgal biomass was raised mixotrophically as well photoautotrophically. For mixotrophic cultivation, molasses was used as additional source of carbon and energy, while for photoautotrophic cultivation, atmospheric CO 2 was the sole source of carbon and energy. The microalgal biomass raised thus was then harvested following 5 days of nitrogen starvation. Mixotrophic cultivation depicted remarkably higher biomass productivity (137.43 ± 13.3 mg L −1 day −1 ) than that of photoautotrophic cultivation (91.57 ± 7.9 mg L −1 day −1 ). About 39% (w/w) of total lipid content was obtained from the dried biomass raised mixotrophically. Lipidomic analysis of the extracted oil depicted higher concentrations of polyunsaturated fatty acids (palmitic and oleic acids). The significant availability of polyunsaturated fatty acids (60 mg g −1 of dried algal biomass) ensured the production of high quality biofuel.

show abstract

Determination of lipid content of oleaginous microalgal biomass by NMR spectroscopic and GC–MS techniques

Cited by 34 publications

References 30 publications

comparison of oil extraction methods for algae by NMR and Chromatographic techniques

comparison of oil extraction methods for algae by NMR and Chromatographic techniques

Lipid quantification techniques for screening oleaginous species of microalgae for biofuel production

Mixotrophic cultivation of Chlorella vulgaris in sugarcane molasses preceding nitrogen starvation: Biomass productivity, lipid content, and fatty acid analyses

Contact Info

Product

Resources

About