Determination of lipids and their oxidation products by IR spectrometry

Dzhatdoeva, A. A.; Polimova, A. M.; Проскурнина, Е. В.; Проскурнин, М. А.; Vladimirov, Yu. A.

doi:10.1134/s1061934816060058

Cited by 9 publications

(7 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…44 The concentration of hydroperoxide groups in linoleic acid samples was determined by infrared (IR) spectroscopy. 45 The height of the absorption peak at the characteristic wavelength served as the analytical signal. The concentration of linoleic acid hydroperoxide (987 cm À1 , -O-Ogroup) in the linoleic acid samples was calculated from the calibration dependence for tert-butyl hydroperoxide (880 cm À1 , -O-Ogroup t-BuOOH).…”

Section: Preparation Of Linoleic Acid and Phosphatidylcholine Hydroperoxidesmentioning

confidence: 99%

“…The concentration of linoleic acid hydroperoxide (987 cm À1 , -O-Ogroup) in the linoleic acid samples was calculated from the calibration dependence for tert-butyl hydroperoxide (880 cm À1 , -O-Ogroup t-BuOOH). 45 The content of hydroperoxide in the samples of oxidised phosphatidylcholine was determined by HPLC with chemiluminescence detection. 46 Analysis of prooxidant activity of CeO 2 nanoparticles by the chemiluminescent method Coumarin 334 (#393002, Sigma-Aldrich) was used as a chemiluminescent probe (CL probe) sensitive to lipid and phospholipid radicals.…”

Section: Preparation Of Linoleic Acid and Phosphatidylcholine Hydroperoxidesmentioning

confidence: 99%

“…[56][57][58] The use of the probe allows for the increase in the CL quantum yield and for the detection of free radical formation. According to a previously published protocol, 45 the concentration of hydroperoxide groups in LOOH samples estimated by IR spectroscopy was 100 AE 12 mmol L À1 .…”

Section: Lipoperoxidase-like Activity Of Ceo 2 Nanoparticlesmentioning

confidence: 99%

See 2 more Smart Citations

New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO₂ nanoparticles

et al. 2021

View full text Add to dashboard Cite

show abstract

Section: Preparation Of Linoleic Acid and Phosphatidylcholine Hydroperoxidesmentioning

confidence: 99%

Section: Preparation Of Linoleic Acid and Phosphatidylcholine Hydroperoxidesmentioning

confidence: 99%

See 1 more Smart Citation

New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO₂ nanoparticles

et al. 2021

View full text Add to dashboard Cite

show abstract

“…However, this leads to a partial loss of acidic lipids, and in some cases also contributes to the formation of very stable emulsions. Purification from non-lipid impurities can be avoided by using IR spectroscopy to analyze the obtained extract [33], since absorption bands corresponding to vibrations of lipid functional groups without preliminary sample treatment are not always informative due to the overlap with water absorption bands [34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Saliva Lipids in Breast and Prostate Cancer by IR Spectroscopy

2021

View full text Add to dashboard Cite

We have developed a method for studying the lipid profile of saliva, combining preliminary extraction and IR spectroscopic detection. The case–control study involved patients with a histologically verified diagnosis of breast and prostate cancer and healthy volunteers. The comparison group included patients with non-malignant pathologies of the breast (fibroadenomas) and prostate gland (prostatic intraepithelial neoplasia). Saliva was used as a material for biochemical studies. It has been shown that the lipid profile of saliva depends on gender, and for males it also depends on the age group. In cancer pathologies, the lipid profile changes significantly and also depends on gender and age characteristics. The ratio of 1458/1396 cm−1 for both breast and prostate cancer has a potential diagnostic value. In both cases, this ratio decreases compared to healthy controls. For prostate cancer, the ratio of 2923/2957 cm−1 is also potentially informative, which grows against the background of prostate pathologies. It is noted that, in all cases, changes in the proposed ratios are more pronounced in the early stages of diseases, which increases the relevance of their study in biomedical applications.

show abstract

“…Однако данные методы являются длительными и дорогостоящими, а также зачастую не предназначены для анализа биологических образцов, содержащих небольшие количества продуктов ПОЛ. Описан метод определения продуктов ПОЛ с применением ИК-спектроскопии, основными достоинствами которого являются экспрессность и высокая чувствительность [8]. В настоящее время внимание большого числа исследователей привлекает слюна в качестве информативного биоматериала, т.к.…”

unclassified

The Use of IR Fourier Spectroscopy of Saliva for Rapid Assessment of the Level of Lipid Peroxidation Products

Bel’skaya

Sarf

2019

BMCRM

View full text Add to dashboard Cite

Infrared spectroscopy of saliva is an express and non-invasive method of analysis, applicable for diagnostics of various diseases and for studying metabolic processes and adaptive changes in the body. The goal of this study was to determine possibility of analyzing the products of lipid peroxidation (LPO) by using IR spectroscopy of saliva on the example of oncological diseases. The study involved 203 patients with lung cancer (n = 40), breast cancer (n = 50) and the control group (n = 113). Saliva samples were collected in the morning after overnight fast. The content of LPO products (conjugated dienes, and trienes, Schiff bases, malonic dialdehyde MDA) was determined in all samples and the IR absorption spectra were recorded in the range of 500–4000 cm–1. In the IR spectra, an increase in the intensity of the absorption bands of lipids was observed; it corresponded to an increase in the total lipid content and correlated with the content of MDA, and a decrease in the intensity of vibrations of oxygen-containing groups, which corresponded to a negative correlation with secondary LPO products. Apparently, on the IR spectra, we simultaneously register both primary, intermediate, and final LPO products. Statistically significant regression equations were obtained, allowing to estimate the content of intermediate LPO products - conjugated triene, and Schiff bases. The proposed method allows to monitor LPO processes, as well as to characterize the direction of the equilibrium shift in these processes.

show abstract

Determination of lipids and their oxidation products by IR spectrometry

Cited by 9 publications

References 12 publications

New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO₂ nanoparticles

New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO₂ nanoparticles

Analysis of Saliva Lipids in Breast and Prostate Cancer by IR Spectroscopy

The Use of IR Fourier Spectroscopy of Saliva for Rapid Assessment of the Level of Lipid Peroxidation Products

Contact Info

Product

Resources

About

Determination of lipids and their oxidation products by IR spectrometry

Cited by 9 publications

References 12 publications

New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO2 nanoparticles

New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO2 nanoparticles

Analysis of Saliva Lipids in Breast and Prostate Cancer by IR Spectroscopy

The Use of IR Fourier Spectroscopy of Saliva for Rapid Assessment of the Level of Lipid Peroxidation Products

Contact Info

Product

Resources

About

New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO₂ nanoparticles

New facets of nanozyme activity of ceria: lipo- and phospholipoperoxidase-like behaviour of CeO₂ nanoparticles