Determination of lipophilic metabolites for species discrimination and quality assessment of nine leafy vegetables

Kim, Tae Jin; Lee, Kyoung Bok; Baek, Seung‐A; Choi, Jaehyuk; Ha, Sun-Hwa; Lim, Sung-Hun; Park, Soo-Yun; Yeo, Yunsoo; Park, Sang Un; Kim, Jae Kwang

doi:10.1007/s13765-015-0119-6

Cited by 27 publications

(30 citation statements)

References 25 publications

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“…Secondary lipophilic compounds such as policosanols, tocopherols, and sterols were extracted as described elsewhere [20]. Lyophilized samples (10 mg) were transferred to 15-mL tubes and extracted with ethanolic ascorbic acid (0.1%, w/v; 3 mL).…”

Section: Extraction Of Secondary Lipophilic Compounds and Their Analymentioning

confidence: 99%

Metabolic Profiling-Based Evaluation of the Fermentative Behavior of Aspergillus oryzae and Bacillus subtilis for Soybean Residues Treated at Different Temperatures

Hyeon

Min

Moon

et al. 2020

Foods

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Soybean processing, e.g., by soaking, heating, and fermentation, typically results in diverse metabolic changes. Herein, multivariate analysis-based metabolic profiling was employed to investigate the effects of fermentation by Aspergillus oryzae or Bacillus subtilis on soybean substrates extracted at 4, 25, or 55 °C. As metabolic changes for both A. oryzae and B. subtilis were most pronounced for substrates extracted at 55 °C, this temperature was selected to compare the two microbial fermentation strategies, which were shown to be markedly different. Specifically, fermentation by A. oryzae increased the levels of most organic acids, γ-aminobutyric acid, and glutamine, which were ascribed to carbohydrate metabolism and conversion of glutamic acid into GABA and glutamine. In contrast, fermentation by B. subtilis increased the levels of most amino acids and isoflavones, which indicated the high activity of proteases and β-glucosidase. Overall, the obtained results were concluded to be useful for the optimization of processing steps in terms of nutritional preferences.

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Section: Extraction Of Secondary Lipophilic Compounds and Their Analymentioning

confidence: 99%

Metabolic Profiling-Based Evaluation of the Fermentative Behavior of Aspergillus oryzae and Bacillus subtilis for Soybean Residues Treated at Different Temperatures

Hyeon

Min

Moon

et al. 2020

Foods

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“…Using the same GC‐qMS conditions as those used in a previous study (Kim and others ), we identified 23 lipophilic metabolites; these were then quantified using the selected ion mode (Table S2). To extract the lipophilic metabolites, fine‐ground samples (0.05 g) were added to 3 mL of ethanol containing 0.1% ascorbic acid (w/v) and 5 μL of 5α‐cholestane (100 μg/mL), which acted as an internal standard, vortexed for 20 s, and placed in a water bath at 85 °C for 5 min.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, metabolomic analysis has been used to direct breeding strategies for the enhancement of optimal combinations of the components of fresh foods. The identification of differences in the metabolite levels in the samples permits a quality‐based classification of species by using metabolite profiling with multivariate analysis (Kim and others ; Kim and others ; Kim and others ). The main application of metabolomics includes identifying metabolic networks connecting primary and secondary metabolism.…”

Section: Introductionmentioning

confidence: 99%

Metabolite Profiling of Peppers of Various Colors Reveals Relationships Between Tocopherol, Carotenoid, and Phytosterol Content

Kim

Choi

Kim

et al. 2017

Journal of Food Science

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“…Freshly powdered samples (300 mg) were used to extract policosanols, tocopherols, and phytosterols. The extraction, and qualitative and quantitative analyses were conducted using previously described methods [17].…”

Section: Extraction Of Lipophilic Compounds and Gc-qms Analysismentioning

confidence: 99%

Metabolic profiling reveals glucose and fructose accumulation in gcr1 knock-out mutant of Arabidopsis

et al. 2019

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The ligands and functions of GCR1, the putative G-protein coupled receptor gene of Arabidopsis thaliana, and its role in metabolism are not well studied. Herein, we determined the contents of different pigments, glucosinolates, and lipophilic and hydrophilic compounds in gcr1 knockout mutant and wild-type plants to investigate the roles of GCR1. Overall, 68 and 58 metabolites were detected using high performance liquid chromatography, gas chromatographyquadrupole mass spectrometry, and gas chromatography-time-of-flight mass spectrometry in 10-day-old seedlings and 24-day-old shoots of mutant and wild-type plants. The levels of glucose and fructose in the gcr1 mutant were significantly higher than those in the wild-type at the two developmental stages. The results of partial least squares discriminant analysis and variable importance in the projection showed that glucose and fructose contributed the most to the separation. These results suggest that GCR1 is linked to glucose sensing and affects glycolysis via cyclic AMP.

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Determination of lipophilic metabolites for species discrimination and quality assessment of nine leafy vegetables

Cited by 27 publications

References 25 publications

Metabolic Profiling-Based Evaluation of the Fermentative Behavior of Aspergillus oryzae and Bacillus subtilis for Soybean Residues Treated at Different Temperatures

Metabolic Profiling-Based Evaluation of the Fermentative Behavior of Aspergillus oryzae and Bacillus subtilis for Soybean Residues Treated at Different Temperatures

Metabolite Profiling of Peppers of Various Colors Reveals Relationships Between Tocopherol, Carotenoid, and Phytosterol Content

Metabolic profiling reveals glucose and fructose accumulation in gcr1 knock-out mutant of Arabidopsis

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