Determination of Lysozyme by Graphene Oxide–Polyethylene Glycol-Based Fluorescence Resonance Energy Transfer

Xie, Yali; An, Jianxin; Shi, Pengzhi; Ye, Nengsheng

doi:10.1080/00032719.2016.1172232

Cited by 13 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the hydrogel H1 without the aptamers showed a significantly high LoD of 37.2 μM ( Figure S4 ) compared with the DNA−H1−960, further demonstrating the decisive rule of the aptamer in this sensing platform. Additionally, the lysozyme detection based on photonic hydrogel DNA−H1−960 provided an excellent LoD compared with other detection method ( Table S2 ) [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 ]. The results demonstrated that the constructed DNA−H1−960 can be a sensitive aptasensor for the detection of lysozyme.…”

Section: Resultsmentioning

confidence: 97%

Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme

Shen

Shi

et al. 2022

Biosensors

View full text Add to dashboard Cite

Responsive two-dimensional photonic crystal (2DPC) hydrogels have been widely used as smart sensing materials for constructing various optical sensors to accurately detect different target analytes. Herein, we report photonic hydrogel aptasensors based on aptamer-functionalized 2DPC poly(acrylamide-acrylic acid-N-tert-butyl acrylamide) hydrogels for facile, label-free and colorimetric detection of lysozyme in human serum. The constructed photonic hydrogel aptasensors undergo shrinkage upon exposure to lysozyme solution through multi-factors cooperative actuation. Here, the specific binding between the aptamer and lysozyme, and the simultaneous interactions between carboxyl anions and N-tert-butyl groups with lysozyme, increase the cross-linking density of the hydrogel, leading to its shrinkage. The aptasensors’ shrinkage decreases the particle spacing of the 2DPC embedded in the hydrogel network. It can be simply monitored by measuring the Debye diffraction ring of the photonic hydrogel aptasensors using a laser pointer and a ruler without needing sophisticated apparatus. The significant shrinkage of the aptasensors can be observed by the naked eye via the hydrogel size and color change. The aptasensors show good sensitivity with a limit of detection of 1.8 nM, high selectivity and anti-interference for the detection of lysozyme. The photonic hydrogel aptasensors have been successfully used to accurately determine the concentration of lysozyme in human serum. Therefore, novel photonic hydrogel aptasensors can be constructed by designing functional monomers and aptamers that can specifically bind target analytes.

show abstract

Section: Resultsmentioning

confidence: 97%

Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme

Shen

Shi

et al. 2022

Biosensors

View full text Add to dashboard Cite

show abstract

“…GO is known to present two main SERS bands located at 1351 cm −1 and 1590 cm −1 corresponding to the D and G bands, respectively. The D band represents disordered sp3 carbon while the G band is associated with the stretching mode of ordered sp2 crystalline graphite-like structures [22]. The spectra of Figure 6b were tentatively normalized to the G band of GO at 1580 cm −1 but the analysis of the normalized spectra provided substantially the same results.…”

Section: Calibration Curve For Fibrillar Hwl On Go-agnc/nsmentioning

confidence: 89%

“…More recently, Pereira-Barros et al proposed an aptamer-based Surface Plasmon Resonance method to selectively detect lysozyme concentrations as low as 0.5 µmol/L [20,21]. Lower detection limits were obtained using more complex procedures involving Fluorescence Energy Transfer [22], luminescent G-quadruplex Iridium(III) complexes [23], field-effect transistor (FET)-based sensing [24], and fluorescent biosensor coupled with gold nanoparticles [25,26]. On the other hand, studies on the detection of the fibrillar form of lysozyme are only in their infancy.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide/Silver Nanoparticles Platforms for the Detection and Discrimination of Native and Fibrillar Lysozyme: A Combined QCM and SERS Approach

et al. 2022

View full text Add to dashboard Cite

We propose a sensing platform based on graphene oxide/silver nanoparticles arrays (GO/AgNPs) for the detection and discrimination of the native and toxic fibrillar forms of an amyloid-prone protein, lysozyme, by means of a combination of Quartz Crystal Microbalance (QCM) and Surface Enhanced Raman Scattering (SERS) measurements. The GO/AgNPs layer system was obtained by Langmuir-Blodgett assembly of the silver nanoparticles followed by controlled adsorption of GO sheets on the AgNPs array. The adsorption of native and fibrillar lysozyme was followed by means of QCM, the measurements provided the kinetics and the mechanism of adsorption as a function of protein concentration as well as the mass and thickness of the adsorbed protein on both nanoplatforms. The morphology of the protein layer was characterized by Confocal Laser Scanning Microscopy experiments on Thioflavine T-stained samples. SERS experiments performed on arrays of bare AgNPs and of GO coated AgNP after native, or fibrillar, lysozyme adsorption allowed for the discrimination of the native form and toxic fibrillar structure of lysozyme. Results from combined QCM/SERS studies indicate a general construction paradigm for an efficient sensing platform with high selectivity and low detection limit for native and amyloid lysozyme.

show abstract

“…This process introduces reactive oxygen-based functional groups, including hydroxy, carboxyl, epoxide, and carbonyl groups, onto the basal planes and borders of the graphene-derived layers, increasing the distance between layers [31,32]. In particular, it has been widely noted that these reactive functional groups are helpful when synthesizing GO-based composite materials, such as the synthesis of RGO-Fe 3 O 4 composite [33], graphene oxide/polyethylene glycol composite [34], Cellulose-Graphite Oxide Composite [35], and so on.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of rGO/CoFe2O4 Composite and Its Magnetorheological Characteristics

Lv,

Gong,

Dong

et al. 2024

Materials

View full text Add to dashboard Cite

In this study, composite particles of rGO/CoFe2O4 were synthesized using a solvothermal method to fabricate a low-density magnetorheological (MR) material with enhanced sedimentation stability. The morphology and crystallographic features of rGO/CoFe2O4 were characterized via SEM, TEM, and XRD, and its magnetic properties were tested using VSM. The MR fluid was formulated by blending rGO/CoFe2O4 particles into silicone oil. Under different magnet strengths (H), a rotational rheometer was used to test its MR properties. Typical MR properties were observed, including shear stress, viscosity, storage/loss modulus, and dynamic yield stress (τdy) following the Herschel–Bulkley model reaching 200 Pa when H is 342 kA/m. Furthermore, the yield stress of the MR fluid follows a power law relation as H increases and the index changes from 2.0 (in the low H region) to 1.5 (in the high H region). Finally, its MR efficiency was calculated to be about 104% at H of 342 kA/m.

show abstract

Determination of Lysozyme by Graphene Oxide–Polyethylene Glycol-Based Fluorescence Resonance Energy Transfer

Cited by 13 publications

References 42 publications

Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme

Multi-Factors Cooperatively Actuated Photonic Hydrogel Aptasensors for Facile, Label-Free and Colorimetric Detection of Lysozyme

Graphene Oxide/Silver Nanoparticles Platforms for the Detection and Discrimination of Native and Fibrillar Lysozyme: A Combined QCM and SERS Approach

Synthesis of rGO/CoFe2O4 Composite and Its Magnetorheological Characteristics

Contact Info

Product

Resources

About