Determination of M/G ratio of propylene glycol alginate sodium sulfate by HPLC with pre-column derivatization

HPLC Method for Microanalysis and Pharmacokinetics of Marine Sulfated Polysaccharides, Propylene Glycol Alginate Sodium Sulfate

“…We determined the M/G ratio of PSS by the NMR technique using its intermediate (low-molecularweight alginate) and desulfurization products. Results showed that the M/G ratios of PSS determined by the HPLC method were in good accordance with those obtained by the traditional nondestructive 1 H-NMR method using desulfurization products (Wu et al 2014).…”

“…The average molecular weight of PSS is about 10 KDa, and it is usually hydrolyzed by chemical degradation methods. Wu et al (2014) studied four methods of hydrolysis of PSS, including dilute sulfuric…”

“…Wang et al (2009) optimized the derivatization conditions for PSS, PMS, and PGS, three marine drugs with different uronic acid components. With the development of analytical techniques, the derivatization method of PSS was further improved (Wu et al 2014).…”

“…The separation conditions of M and G derivatives were investigated by changing the pH value of the phosphate buffer, the proportion of acetonitrile, the flow rate, and loading quantity of the sample (Wu et al 2014). The chromatogram was shown in Fig.…”

“…Among them, M/G ratio of PSS is one of the most important structural factors (Wu et al 2014) for its activities and identification. There is an urgent need to develop a reliable and sensitive method for determination of the M/G ratio of PSS for quality control and clinical purposes.…”