Determination of MAG-Camptothecin, a new polymer-bound Camptothecin derivative, and free Camptothecin in dog plasma by HPLC with fluorimetric detection

A two-step pH-dependent liquid–liquid extraction combined with HPLC-fluorescence method for the determination of 10-hydroxycamptothecin in mouse liver tissue

“…The pharmacokinetics of CPTs in plasma samples by HPLC with fluorescence detection has been well established (Guo et al, 2011a;Liu et al, 2008;Loh & Ahmed, 1990;Sun et al, 2004). The biological samples were prepared using relatively simple approaches such as protein precipitate with acetonitrile (ACN)/methanol (Fraier et al, 2000;Guo et al, 2003), SPE (D'Esposito et al, 2008;Fraier et al, 2000) or extraction with organic solvents using a conventional LLE (Koshkina et al, 1999;Liu et al, 2008;Sun et al, 2004;Zhao et al, 2006). However, the same sample preparation methods for plasma samples were used for tissue samples in most cases (Koshkina et al, 1999;Wu & Zhang, 2001).…”

Validation study of a method for assaying DE-310, a macromolecular carrier conjugate containing an anti-tumor camptothecin derivative, and the free drug in tumor tissue by high performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry

“…Previous work on a polymer-bounded camptothecin derivative (MAG-CPT) has demonstrated that the total levels of CPT (free CPT plus polymer-bounded CPT) are determined after alkaline-mediated hydrolysis of MAG-CPT (Fraier et al, 2000;Schoemaker et al, 2001). The lower limits of quantitation (LLOQs) of this method were 100 ng/mL of total and 1 ng/mL of free CPT for 50 and 250 µL of human plasma.…”

Validation study of assay method for DE-310, a novel polymer-bound camptothecin derivative, and the free drug in mouse plasma by liquid chromatography with fluorimetric detection

“…Some researchers have developed analytical methods for studying polymer-bound anticancer drug. Previous work on a polymer-bound camptothecin derivative (MAG-CPT) has demonstrated that the total levels of CPT (free plus polymer-bound) are determined after alkaline-mediated hydrolysis of MAG-CPT [9,10]. The LLOQs of this method were 100 ng/ml of total and 1 ng/ml of free CPT for 50 and 250 l human plasma, respectively.…”