Determination of major triacylglycerol components of polyunsaturated specialty oils using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry

Ayorinde, Folahan O.; Eribo, Broderick E.; Balan, Kannan V.; Johnson, James H.; Wan, L. W.

doi:10.1002/(sici)1097-0231(19990530)13:10<937::aid-rcm590>3.0.co;2-t

Cited by 47 publications

(43 citation statements)

References 15 publications

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“…Flax meal neutral lipids contained 95-98% triacylglycerides (TAGs) (Oomah and Mazza, 1998). Trilinolenate (LnLnLn; 35%) was the main TAG (Ayorinde, 2000;Holcapek et al, 2003). Trilinolenate (LnLnLn; 35%) was the main TAG (Ayorinde, 2000;Holcapek et al, 2003).…”

Section: Flaxseed Oil Compositionmentioning

confidence: 99%

Hemp and flaxseed oil

Dunford

2015

Specialty Oils and Fats in Food and Nutrition

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Section: Flaxseed Oil Compositionmentioning

confidence: 99%

Hemp and flaxseed oil

Dunford

2015

Specialty Oils and Fats in Food and Nutrition

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“…Mass spectrometric analysis of intact lipid molecules has been performed using chemical ionization,4–8 fast atom bombardment (FAB),9–13 electrospray,14–22 and MALDI 23–32. Electrospray, FAB and MALDI have been the most widely used soft ionization methods.…”

mentioning

confidence: 99%

“…The routine application of these techniques to large numbers of samples is still complicated, due to their intolerance to impurities (ESI) and varied instrumental response to different lipid classes/molecular species (ESI, FAB) 14,. 16,, 32,, 33 MALDI analysis of lipid molecules is generally more sensitive and less affected by impurities and polarities of the analytes than ESI 25,. 26,, 28,, 33 Although seldom used to provide detailed structural information for particular molecular species,28,, 31,, 35 MALDI is potentially an excellent analytical method for rapidly screening lipid components in biological matrices 35…”

mentioning

confidence: 99%

“…28,, 30 In the positive mode, lipids differed in their affinity for sodium ions. For instance, TAGs and diacylglycerols (DAGs) were only detected as sodium adducts and not as protonated molecules, when 2,5‐dihydroxybenzoic acid (DHB) was used as matrix 25,. 26,, 28,, 31 For phospholipids such as PE and PS, protonation and the multiple additions of sodium ions were simultaneously observed 23,.…”

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confidence: 99%

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Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry of lipids: ionization and prompt fragmentation patterns

Al‐Saad

Zabrouskov

Siems

et al. 2002

Rapid Comm Mass Spectrometry

128

111

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Ionization and prompt fragmentation patterns of triacylglycerols, phospholipids (PLs) and galactolipids were investigated using matrix-assisted laser desorption/ionization (MALDI). Positive ions of non-nitrogen-containing lipids appeared only in the sodiated form, while nitrogen-containing lipids were detected as both sodiated and protonated adducts. Lipids containing acidic hydroxyls were detected as multiple sodium adducts or deprotonated ions in the positive and negative modes, respectively, with the exception of phosphatidylcholines. The positive MALDI spectra of triacylglycerols contained prompt fragments equivalent to the loss of RCOO(-) from the neutral molecules. Prompt fragment ions [PL-polar head](+) were observed in the positive MALDI spectra of all phospholipids except phosphatidylcholines. The phosphatidylcholines produced only a minor positive fragment corresponding to the head group itself (m/z 184). Galactolipids did not undergo prompt fragmentation. Post-source decay (PSD) was used to examine the source of prompt fragments. PSD fragment patterns indicated that the lipid prompt fragment ions did not originate from the observed molecular ions (sodiated or protonated), and suggested that the prompt fragmentation followed the formation of highly unstable, probably protonated, precursor ions. Pathways leading to the formation of prompt fragment ions are proposed.

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“…Palmitic (9-13%), stearic (5-6%), and α-linolenic (1%) acids are also present (Tashiro et al, 1990;Kamal-Eldin and Appelqvist, 1994;Bahkali et al, 1998;Mohamed and Awatif, 1998;Ayorinde et al, 1999). Palmitic (9-13%), stearic (5-6%), and α-linolenic (1%) acids are also present (Tashiro et al, 1990;Kamal-Eldin and Appelqvist, 1994;Bahkali et al, 1998;Mohamed and Awatif, 1998;Ayorinde et al, 1999).…”

Section: General Characteristicsmentioning

confidence: 99%

Other natural antioxidants – rice bran oil, sesame oil, rosemary extract, flavonoids

Hall

2012

Lipids for Functional Foods and Nutraceuticals

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Determination of major triacylglycerol components of polyunsaturated specialty oils using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry

Cited by 47 publications

References 15 publications

Hemp and flaxseed oil

Hemp and flaxseed oil

Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry of lipids: ionization and prompt fragmentation patterns

Other natural antioxidants – rice bran oil, sesame oil, rosemary extract, flavonoids

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