Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection

Mitrowska, Kamila; Posyniak, Andrzej; Żmudzki, J.

doi:10.1016/j.chroma.2005.07.004

Cited by 142 publications

(63 citation statements)

References 11 publications

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“…Similar results were reported by Culp et al [36] who found significant increase in both serum AST and ALT of rats fed on MG dye for 4weeks, they attributed these changes in liver function to hepatocellular impairment which subsequently caused the release of greater levels of intracellular enzymes into the blood. On the other hand Mittelstaedt et al found low level of ALT, AST and ALP in liver tissue after MG treatment.…”

Section: Discussionsupporting

confidence: 78%

Can Malachite Green Concentrations Cause Hepatic Toxic Effects in Adult Albino Rats: Histological, Biochemical and Immunohistochemical

NAM¹,

Elgendy²

2016

J Cytol Histol

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Malachite green (MG) has been widely used as the most efficacious antifungal agent in the fish farming industry. The aim of this study is to evaluate hepatotoxicity of different MG concentrations in adult albino rats. Forty five adult male albino rats were equally divided into 3 groups, each contains 15 rats. Group I (control), Group II (received daily dose of 3ug/kg b.wt of MG by oral gavage): divided into three subgroup equally according to time of taken sample and Group III (received daily gavage dose of 60ug/kg b.wt of MG by oral gavage): divided into three subgroup equally according to time of taken sample (Samples were taken 2 and 4 weeks from the start of the experiment and 2 weeks after drug stoppage (wash out). Hepatotoxicity was evaluated by biochemical, histopathological and immunohistochemical study. MG caused an increase of alanine aminotransferase, aspartate amino transferase (ALT and AST), alkaline phosphatase (ALP) levels, and decrease in total protein in animals treated with MG as compared to control. These biochemical alterations were confirmed by the presence of different histological changes as degeneration of hepatocytes with congested dilated central vein , mononuclear inflammatory cells infiltration with significant increase in caspase-3 expression with decrease in survivin expression and this changes were highly significant in rats treated with higher doses than other groups and persist even after 2 weeks from MG stoppage .We can conclude that MG and its residues in the edible tissue of fish could not be ignored due to their suspected hepatotoxicity with apoptotic changes even at lower doses. This leads us to put strict limitations on its use in the fish farming industry in Egypt.

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Section: Discussionsupporting

confidence: 78%

Can Malachite Green Concentrations Cause Hepatic Toxic Effects in Adult Albino Rats: Histological, Biochemical and Immunohistochemical

NAM¹,

Elgendy²

2016

J Cytol Histol

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“…The LMG levels in the three species were correlated significantly with lipid content in the fish tissue. Earlier findings indicated that the distribution of LMG was related to the fat content of fish, such as channel catfish and rainbow trout (Poe and Wilson, 1983;Máchová et al, 1996;Tarbin et al, 1998;Halme et al, 2004;Mitrowska et al, 2005). Bauer et al (1988) found that the half-life of LMG in muscle was positively correlated with muscle lipid content and ranged from 10 to 40 days in rainbow trout.…”

Section: Discussionmentioning

confidence: 99%

Distribution and depuration of the potentially carcinogenic malachite green in tissues of three freshwater farmed Chinese fish with different food habits

2009

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“…A number of laboratory based analytical techniques have been developed for determination of malachite green, such as high performance liquid chromatography (HPLC) (Mitrowska et al 2005;Bajc et al 2007;Furusawa 2014;Swarbrick et al 1997;Long et al 2008;Bergwerff et al 2003;Xie et al 2013) and liquid chromatography-tandem mass spectrometry (Nebot et al 2013;Lopez-Gutierrez et al 2013;Hidayah et al 2013). These methods can achieve high sensitivity and excellent accuracy.…”

Section: Introductionmentioning

confidence: 99%

Determination of Malachite Green in Aquaculture Water by Adsorptive Stripping Voltammetry

Zhu

Honeychurch

et al. 2015

Analytical Letters

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Determination of malachite green and leucomalachite green in carp muscle by liquid chromatography with visible and fluorescence detection

Cited by 142 publications

References 11 publications

Can Malachite Green Concentrations Cause Hepatic Toxic Effects in Adult Albino Rats: Histological, Biochemical and Immunohistochemical

Can Malachite Green Concentrations Cause Hepatic Toxic Effects in Adult Albino Rats: Histological, Biochemical and Immunohistochemical

Distribution and depuration of the potentially carcinogenic malachite green in tissues of three freshwater farmed Chinese fish with different food habits

Determination of Malachite Green in Aquaculture Water by Adsorptive Stripping Voltammetry

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