Determination of malonaldehyde and formaldehyde formed from fatty acid ethyl esters upon microwave and thermal heating

Effects of dietary enrichment with alpha-tocopherol acetate and post-harvest filleting on lipid oxidation and flesh quality of tropical farmed barramundi (Lates calcarifer)

“…Lipid oxidation was measured by a distillation-colorimetric technique, the 2-thiobarbituric acid method (TBARS) modified from Wong et al (1991) as used in previous studies to measure lipid oxidation in teleosts (Frigg et al, 1990;Jittinandana et al, 2006;Musgrove et al, 2011;Ruff et al, 2002). Analyses were performed at the Lincoln Marine Science Centre (Port Lincoln, School of Biological Sciences, Flinders University).…”

Analytical methods for trace levels of reactive carbonyl compounds formed in lipid peroxidation systems

“…Table 1 shows typical RCCs formed from lipid peroxidation and their physical constants. Some secondary lipid peroxidation products, such as MA, have been widely used as biomarkers for investigating in vivo and in vitro oxidative damage [54][55][56][57][58][59]61,63,64,[68][69][70]. Even though numerous lipid peroxidation products have been identified, only a few chemicals have been satisfactorily used as biomarkers of oxidative damages.…”

“…MA reacts readily with N-methyl hydrazine under mild conditions (at room temperature and pH 7). Trace levels of MA were successfully detected in various matrices associated with lipid peroxidation, including fatty acids [54,68,70] cod liver oil [55,157], dietary oils [65], and biological samples from animals [145,159]. For example, cod liver oil produced 3.8-190.2 nmol/mg of MA upon UV irradiation [55].…”

“…For example, cod liver oil produced 3.8-190.2 nmol/mg of MA upon UV irradiation [55]. Microwave irradiation was proved to induce lipid peroxidation in a study involving the analysis of MA formed from fatty acid ethyl esters [134].…”

Interaction of malondialdehyde with biological molecules — new trends about reactivity and significance

“…Another ion-pairing HPLC method achieves a simultaneous separation of MDA, ascorbic acid and adenine nucleotide derivatives (Lazzarino et al, 1991). A recent gas chromatographic method (Tamura & Shibamoto, 1991;Wong et al, 1991) reacts MDA with N-methylhydrazine to give l-methylpyrazole (Umano et al, 1988) which is analysed on a fused silica capillary column using a nitrogen-phosphorus detector.…”