Determination of Malonaldehyde in Alzheimer’s Disease: A Comparative Study of High-Performance Liquid Chromatography and Thiobarbituric Acid Test

Bermejo, Paulina; Gómez-Serranillos, M. Pilar; Santos, José Victor de Oliveira; Pastor, Eitel; Gil, Pedro; Martín‐Aragón, Sagrario

doi:10.1159/000213853

Cited by 39 publications

(21 citation statements)

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“…MDA levels were highest in AD (897 ± 84) and in VA (712 ± 81) that in the control group (536 ± 78). This is in accordance with the results obtained by Bermejo et al [50] in AD patients. Furthermore, in this study we have observed that MDA levels increase with age, which is in accordance to the results obtained by Gil et al [51] in a Spanish population.…”

Section: Discussionsupporting

confidence: 94%

Lipid Peroxidation and Antioxidant Enzyme Activities in Vascular and Alzheimer Dementias

et al. 2007

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It has been reported that oxidative stress may play a role in the pathogenesis of dementia of the Alzheimer type (AD) and the cerebral ischemia which causes vascular dementia (VD). We measured malondialdehyde (MDA) levels and superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) activities in blood samples from patients with AD and VD and in healthy non-demented controls (CTR) which similar ages to the patients, in order to evaluate the degree of oxidative stress in patients with AD and VD. A sample of 150 subjects consisting of 50 patients with AD; 50 patients with VD and 50 CTR, aged from 65 to 85 years on, was analyzed. Most of the changes observed were in SOD activity and MDA levels. Catalase activity were least affected. Significant differences were observed in SOD and GR activity between males and females in CRT and in patients with AD, but not in VD. We have found a decrease in antioxidant enzymes activities (SOD, CAT, GPx and GR) in patients with AD and VD and significant differences were observed between CRT and AD patients for ages from 65 to 74, 75 to 84 and from 85 years to 94 years in SOD activity and MDA levels (P < 0.001). MDA levels increase with age in VD, AD and CTR. No significant variation with respect to sex were detected, but significant variations in MDA levels were detected between CRT and patients with VD and AD (P < 0.001). We conclude that oxidative stress plays an important role in the brain damage for both AD and VD, being observed higher levels of oxidative stress for AD that for VD.

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Section: Discussionsupporting

confidence: 94%

Lipid Peroxidation and Antioxidant Enzyme Activities in Vascular and Alzheimer Dementias

et al. 2007

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“…This observation is different from previous publications that reported signifi cant differences in MDA levels of erytrocytes of AD patients as compared with the controls (Bermejo et al 1997). In other studies, the ex -vivo susceptibility to lipid peroxidation was higher in mild and moderate AD with respect to controls, but not in severe AD patients (Galbusera et al 2004).…”

Section: Discussioncontrasting

confidence: 97%

Cognitive impairment and Alzheimer’s disease: Links with oxidative stress and cholesterol metabolism

Maccioni¹

2008

NDT

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Abstract:Oxidative stress has been implicated in the progression of a number of neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease and amyotrophic lateral sclerosis. We carried out an in-depth study of cognitive impairment and its relationships with oxidative stress markers such as ferric-reducing ability of plasma (FRAP), plasma malondialdehyde and total antioxidative capacity (TAC), as well as cholesterol parameters, in two subsets of subjects, AD patients (n = 59) and a control group of neurologically normal subjects (n = 29), attending the University Hospital Salvador in Santiago, Chile. Cognitive impairment was assessed by a set of neuropsychological tests (Mini-Mental State Examination, Boston Naming Test, Ideomotor Praxia by imitation, Semantic Verbal Fluency of animals or words with initial A, Test of Memory Alteration, Frontal Assessment Battery), while the levels of those oxidative stress markers and cholesterol metabolism parameters were determined according with standard bioassays in fresh plasma samples of the two subgroups of patients. No signifi cant differences were observed when the cholesterol parameters (low-, high-density lipoprotein, total cholesterol) of the AD group were compared with normal controls. Interestingly, a correlation was evidenced when the levels of cognitive impairment were analyzed with respect to the plasma antioxidant capacity (AOC) of patients. In this context, the subset of subjects exhibiting cognitive impairment were divided into two subgroups according with their Global Dementia Scale performance: a subgroup with mild AD and a subgroup with moderate to severe AD. Signifi cant differences in AOC were found between subgroups. The different correlations between cognitive impairment of subgroups of subjects with the oxidative stress profi le are discussed in the context of AD pathogenesis.

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“…Several studies analyze some peroxidation markers Gil/Fariñas/Casado/Ló pez-Ferná ndez in common pathologies for the old, such as diabetes mellitus [28], congestive heart failure [29], Alzheimer's [30], or allergic diseases [31], but they do not analyze healthy old people. Our study analyzes healthy old people, that is, satisfactory ageing.…”

Section: Discussionmentioning

confidence: 99%

Malondialdehyde: A Possible Marker of Ageing

Gil¹,

Fariñas²,

Casado³

et al. 2002

Gerontology

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Background: Numerous recent studies have suggested that oxidative damage may be important in the ageing process, and lipid peroxidation is an important biological consequence of oxidative cellular damage. Objective: The aim of this work was to analyze the activities of the two protective enzymes, superoxide dismutase (SOD) and catalase (CAT), and the levels of malondialdehyde (MDA) to examine the relationship between the ageing process and defence antioxidant and lipid peroxidation. Method: SOD activity was measured in red blood cells using the Minami and Yoshikawa method; CAT activity was measured in hemolysates by the Aebi method, and MDA levels were measured in erythrocytes by high-performance liquid chromatography. Results: SOD activity shows statistically significant differences between newborns and the rest of the sample (ANOVA p < 0.001; Student-Newman-Keuls test p < 0.001). CAT activity did not show significant differences between the age groups. We observed statistically significant differences in MDA levels between the different groups (ANOVA p < 0.001; Student-Newman-Keuls test p < 0.05). In the regression analysis and rectilinear/curvilinear adjustment compared to age, SOD and CAT showed coefficients close to zero (SOD linear = 0.16; SOD exponential = 0.15; CAT linear = 0.056; CAT exponential = 0.068), indicating in that way their independence from age. Only MDA obtained a regression coefficient superior to 0.75 (p < 0.05). The best adjustment was reached through an exponential expression, giving the following parametric relation: MDA = 103.117e^0.0021.AGE. No statistically significant variation in SOD and CAT activity and MDA levels, related to sex could be demonstrated. Conclusions: Our data show that old age is associated with an increase in systemic oxidative stress.

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Determination of Malonaldehyde in Alzheimer’s Disease: A Comparative Study of High-Performance Liquid Chromatography and Thiobarbituric Acid Test

Cited by 39 publications

References 11 publications

Lipid Peroxidation and Antioxidant Enzyme Activities in Vascular and Alzheimer Dementias

Lipid Peroxidation and Antioxidant Enzyme Activities in Vascular and Alzheimer Dementias

Cognitive impairment and Alzheimer’s disease: Links with oxidative stress and cholesterol metabolism

Malondialdehyde: A Possible Marker of Ageing

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Determination of Malonaldehyde in Alzheimer’s Disease: A Comparative Study of High-Performance Liquid Chromatography and Thiobarbituric Acid Test

Cited by 39 publications

References 11 publications

Lipid Peroxidation and Antioxidant Enzyme Activities in Vascular and Alzheimer Dementias

Lipid Peroxidation and Antioxidant Enzyme Activities in Vascular and Alzheimer Dementias

Cognitive impairment and Alzheimer&rsquo;s disease: Links with oxidative stress and cholesterol metabolism

Malondialdehyde: A Possible Marker of Ageing

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Cognitive impairment and Alzheimer’s disease: Links with oxidative stress and cholesterol metabolism