Determination of medroxyprogesterone acetate residues by CE immunoassay with chemiluminescence detection

Peng, Chifang; Huo, Tieming; Liu, Liqiang; Chu, Xiaogang; Xu, Chuanlai

doi:10.1002/elps.200600290

Cited by 21 publications

(18 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Basically, this CL reaction has been used in the determination of medroxyprogesterone acetate (MPA) [121] in pork tissues. MPA is a synthetic hormone often used in veterinary medicine to speed up fattening and can be a potential hazard for consumers.…”

Section: Food and Environmental Applicationsmentioning

confidence: 99%

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

2010

View full text Add to dashboard Cite

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresisThe present review presents the state of the art of the developments, key strategies and analytical applications of chemiluminescence detection coupled to CE (CE-CL). Different parts considering the most common CL systems have been included, such as the tris(2,2 0 -bipyridine)ruthenium(II) system, the luminol and derivatives reaction, the peroxyoxalate CL or direct oxidations. New advances in homemade configurations and applications in different fields such as clinical, pharmaceutical, environmental and food analysis have been included. The focus is on studies which appeared from 2000 to the end of 2009.

show abstract

Section: Food and Environmental Applicationsmentioning

confidence: 99%

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

2010

View full text Add to dashboard Cite

show abstract

“…In addition, medroxyprogesterone acetate (MPA), a tumor marker, carbohydrate antigen 15-3 (CA15-3), and a tumor Ag, CA 125 have been detected by the CE immunoassay coupled with a HRP label. [23][24][25] The sensitivity of CL makes it very promising for microchip CE-detection systems. Tsukagoshi et al 26 developed a micro total analysis system (m-TAS) incorporating the CL reaction of isoluminol isothiocyanato-microperoxidase-hydrogen peroxide.…”

Section: ·4 Enzyme-based CL Techniques 2·4·1 Hrpmentioning

confidence: 99%

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Fan

Cao

et al. 2009

ANAL. SCI.

View full text Add to dashboard Cite

IntroductionCL is the production of electromagnetic radiation by a chemical reaction. The CL intensity is directly proportional to the concentration of a limiting reactant involved in the CL reaction. CL has been exploited for its use in a wide range of applications due to its high sensitivity, wide calibration range, and suitability for miniaturization. Protein and DNA analyses have recently attracted much attention. Many different types of techniques, such as electrochemical, optical, radiochemical, and piezoelectronic methods have been applied for protein and DNA analyses. To provide an overview of CL detection assays for DNAs and proteins, we have summarized their essential techniques. Monoplex and multiplex assays are described, and their current reports are intended to provide an overview of the CL techniques currently employed in numerous laboratories. Monoplex AssayA monoplex assay aims to measure the presence of a single analyte in any given sample, in which nanoparticle, DNA enzyme, horseradish peroxidase (HRP), alkaline phosphatase (AP) and acridinium ester are commonly used as labels. 2·1 Nanoparticle-based CL techniquesNanoparticles offer excellent prospects for DNA detection and immunoassay, owing to their many attractive properties. Compared to existing labels, nanoparticles are more stable and cheaper, and easier to be purified. In addition, they indicate faster binding kinetics with high sensitivity and a high reaction rate for many types of monoplex assays, ranging from immunoassays to DNA detection. 2·1·1 Gold nanoparticlesColloidal gold labels are ideal in biotechnological systems for several reasons. Firstly, they can be readily prepared in a wide range of sizes, from approximately 2 nm to above 100 nm. Secondly, the biochemical activity of the labeled biomolecules can be retained when colloidal gold is coupled to the biomolecules and, thirdly, colloidal gold labels can be easily visualized as dense structures within biological entities using transmission electron microscopy. As a biological tag, colloidal gold has been employed in several CL detection systems. 2·1·1·1 Gold nanoparticle-based CL immunoassay. The Au 3+ -luminol reaction, in which 10 -9 M Au 3+ ions can be sensitively detected, is a classic CL reaction. Thousands of Au atoms are contained within the gold nanoparticles; for example, 2.3 ¥ 10 5 Au atoms are theoretically contained in a 20-nm spherical gold particle and, consequently, picomolar detection limits can be attained by employing oxidative gold metal dissolution in acidic solutions. Our group 1,2 reported a sensitive CL immunoassay based on a colloidal gold label after oxidative gold metal dissolution (using 0.01 M HCl-0.5 M NaCl-0.5 mM Br2), using a simple and sensitive luminol-CL reaction (Fig. 1). Reviews Chemiluminescence Platforms in Immunoassay and DNA AnalysesAiping FAN,* Zhijuan CAO,* Huan LI,* Masaaki KAI,** and Jianzhong LU* † *School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China ** Faculty of Pharmaceutical Sciences, Graduate Scho...

show abstract

“…It has been reported worldwide that some synthetic steroidal hormones have been used as growth promoters in animal breeding (Barel et al, 2006;Hooijerink, Van Bennekom, & Nielen, 2003;Peng, Xu, Jin, Chu, & Wang, 2006b;Peng, Huo, Liu, Chu, & Xu, 2007;Peng et al, 2008Peng et al, , 2009Xu, Peng, Hao, Jin, & Chu, 2006;Yuan, Xu, & Peng, 2008;Zhou, 2003). The potential harm of these hormone residues in foods and the environment has been recognised publicly.…”

Section: Introductionmentioning

confidence: 97%

“…MPA is also considered as a hormone which can speed up fattening. The presence of trace MPA in animal tissues poses a potential hazard for normal consumers Peng et al, 2007). The determination of MPA concentration in the samples of serum, urine and tissue is of great importance in daily life in China.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive signal amplified immunoassay of medroxyprogesterone acetate (MPA) using the atomic absorption of silver deposited on the surface of gold nanoparticles

Cui

Chu

et al. 2010

Food and Agricultural Immunology

View full text Add to dashboard Cite

An ultrasensitive determination method was developed to detect the trace medroxyprogesterone acetate (MPA) residues in food. The operation procedure was mostly the same as the traditional enzyme-linked immunosorbent assay method. The gold nanoparticle labelled goat anti-rabbit antibody was used here to take the traditional enzyme labelled antibody. Then the gold labelled antibody was used in the immunoassay as usual. The silver was deposited on the surface of the gold nanoparticles on the second antibody. The quantity of the silver on the surface of the gold nanoparticles was dissolved and analysed by the atomic absorption spectrum. Accordingly, the trace analyte MPA could be determined. The range of the determination was from 0.63 to 39.8 ng/ml and the limit of the detection was 0.25 ng/ml.

show abstract

Determination of medroxyprogesterone acetate residues by CE immunoassay with chemiluminescence detection

Cited by 21 publications

References 24 publications

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Ultrasensitive signal amplified immunoassay of medroxyprogesterone acetate (MPA) using the atomic absorption of silver deposited on the surface of gold nanoparticles

Contact Info

Product

Resources

About