Determination of melatonin and its precursors and metabolites using capillary electrophoresis with UV and fluorometric detection

Poboży, Ewa; Michalski, Aleksander; Sotowska-Brochocka, Joanna; Trojanowicz, Marek

doi:10.1002/jssc.200500095

Cited by 24 publications

(11 citation statements)

References 33 publications

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“…The results from regression analysis are shown in Table 3. The linearity of the calibration curves for all six analytes was excellent over the range of 0.06-60 mmol/L with LODs of 9.7 to 41.8 nmol/L or 2.0 to 9.7 ng/mL, which provide about 30-to 250-fold improvement over UV and native fluorescence detection of MT and some indoleamines [23].…”

Section: Precision Linearity and Detection Limitsmentioning

confidence: 98%

“…Although several kinds of detectors, such as UV, diode array, fluorometric detector and electrochemical detector, have been used for the detection of MT and some indoles after CE or MEKC separation [13][14][15][16][17][18][19][20][21][22], the most widely used detection mode is UV with a relatively low detection sensitivity (,mM level). It was also found that direct determination of MT and its precursors and metabolites by CE-fluorometric detection in biological samples was impossible due to high co-extracted signals of matrices and small response signals of analytes [23].…”

Section: Introductionmentioning

confidence: 99%

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Micellar electrokinetic capillary chromatography for fast separation and sensitive determination of melatonin and related indoleamines using end‐column amperometric detection

Zhang

et al. 2006

Electrophoresis

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MEKC was used in conjunction with end-column amperometric detection (AD) at a carbon disc electrode (0.3 mm diameter) for the selective and sensitive determination of melatonin and its five related indoleamines including its precursors and metabolites in the pineal gland. The introduction of a sample stacking technique in injection and the buffer additive SDS in the buffer solution system provided the rapid and sensitive analysis. Optimal buffer conditions (10 mmol/L phosphate containing 20 mmol/L SDS, pH 7.2), detection potential (+1.0 V vs. Ag/AgCl), and electrokinetic injection 10 s with the separation voltage of 24 kV were employed to achieve the baseline separation of six pineal hormones within 15 min. The peak currents and the analyte concentrations have a good linear relationship over the range of 6.0 x 10(-8) 6.0 x 10(-5 )mol/L. The detection limits for six pineal hormones by AD are 9.7 to 41.8 nmol/L (equal to 2.0 to 9.7 ng/mL) (S/N = 3), respectively. It is proved to provide about 30- to 250-fold improvement over UV, and be comparable with the sensitive fluorescence detection, which needs pre-column derivatization. The proposed method has been applied for analysis of melatonin and related indoleamines in rat pineal glands. A very simple sample pretreatment procedure, merely involving the homogenization step in perchloric acid, was enough to achieve recoveries in the range of 71 to 127% for all the analytes in the pineal gland.

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Section: Precision Linearity and Detection Limitsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Micellar electrokinetic capillary chromatography for fast separation and sensitive determination of melatonin and related indoleamines using end‐column amperometric detection

Zhang

et al. 2006

Electrophoresis

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“…In this regard, several amino acids were measured using CE in a short period of time [103]. Several other metabolites profiled from urine, plasma, saliva, or other organisms have also been reported [104][105][106][107][108][109][110].…”

Section: Ce In Metabolomicsmentioning

confidence: 99%

CE at the omics level: Towards systems biology – An update

Song

Babar

et al. 2007

Electrophoresis

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This review provides an updated overview of recent developments and applications of CE based on previously published reports in the field of omic research. The increased number of published articles on omics shows that the field is growing and attracting the attention of many life science researchers. Due to developments in the omics sciences, many researchers have been studying systems biology, in which biological events in organisms are systematically interpreted through the combination of complex measurements from various methods resulting in high-throughput data. Given the challenges of such complex forms of analysis, CE is a strong candidate for generating omics data useful for acquiring the qualitative and quantitative knowledge necessary for systems-level investigation. By emphasizing CE for systems biology, this review will discuss and focus on the applicability of CE to systems-based analytical data at the genomic, transcriptomic, proteomic, and metabolomic levels from 2005 to the present.

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“…The detection limit of melatonin with CE is comparable with the data obtained by HPLC methods reported previously. Recently, for the separation of melatonin from related compounds, CE with micellar electrokinetic chromatography was applied [125, 126, 128, 129]. This technique permitted the effective separation of melatonin and its precursors or metabolites.…”

Section: Introductionmentioning

confidence: 99%

Measurement of melatonin in body fluids: Standards, protocols and procedures

et al. 2010

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Background and PurposeThe circadian rhythm of melatonin in saliva or plasma, or of the melatonin metabolite 6-sulfatoxymelatonin (a6MTs) in urine, is a defining feature of suprachiasmatic nucleus (SCN) function, the body’s endogenous oscillatory pacemaker. The primary objective of this review is to ascertain the clinical benefits and limitations of current methodologies employed for detection and quantification of melatonin in biological fluids and tissues.Data IdentificationA search of the English-language literature (Medline) and a systematic review of published articles were carried out.Study SelectionArticles that specified both the methodology for quantifying melatonin and indicated the clinical purpose were chosen for inclusion in the review.Data ExtractionThe authors critically evaluated the methodological issues associated with various tools and techniques (e.g. standards, protocols, and procedures).Results of Data SynthesisMelatonin measurements are useful for evaluating problems related to the onset or offset of sleep and for assessing phase delays or advances of rhythms in entrained individuals. They have also become an important tool for psychiatric diagnosis, their use being recommended for phase typing in patients suffering from sleep and mood disorders. Additionally, there has been a continuous interest in the use of melatonin as a marker for neoplasms of the pineal region. Melatonin decreases such as found with aging are or post pinealectomy can cause alterations in the sleep/wake cycle. The development of sensitive and selective methods for the precise detection of melatonin in tissues and fluids has increasingly been shown to have direct relevance for clinical decision making.ConclusionsDue to melatonin’s low concentration, as well as the coexistence of numerous other compounds in the blood, the routine determination of melatonin has been an analytical challenge. The available evidence indicates however that these challenges can be overcome and consequently that evaluation of melatonin's presence and activity can be an accessible and useful tool for clinical diagnosis.

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Determination of melatonin and its precursors and metabolites using capillary electrophoresis with UV and fluorometric detection

Cited by 24 publications

References 33 publications

Micellar electrokinetic capillary chromatography for fast separation and sensitive determination of melatonin and related indoleamines using end‐column amperometric detection

Micellar electrokinetic capillary chromatography for fast separation and sensitive determination of melatonin and related indoleamines using end‐column amperometric detection

CE at the omics level: Towards systems biology – An update

Measurement of melatonin in body fluids: Standards, protocols and procedures

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