Determination of meropenem in plasma by high–performance liquid chromatography and a microbiological method

Abstract

A rapid and selective high-performance liquid chromatographic (HPLC) method for the quantitative determination of meropenem in plasma is described. The drug was separated from plasma after plasma protein precipitation with 15% of trichloroacetic acid. The mobile phase consisted of acetonitrile-water-glacial acetic acid (21.2, 78 and 0.8% v/v, respectively) delivered at a flow rate of 1.2 ml/min. Meropenem was quantified using ultraviolet detection at 296 nm. Meropenem and the internal standard (pheniramine) were well separated from plasma components. The drug could be assayed by the HPLC method in the presence of its analogue, imipenem. The detection limit in plasma was 25 ng/ml of meropenem. The results were compared with those of agar for a microbiological diffusion method using Escherichia coli ATCC 25922 as the test organism. The sensitivity of the microbiological assay was less than 5 ng/ml, but this decreased at higher concentrations. Both methods were applied to the determination of the drug in aqueous solutions and in plasma.

Penetration of moxifloxacin into the human aqueous humour after oral administration

“…High performance liquid chromatography (HPLC) which is nowadays widely used as a method of choice to assay drug levels, was not available in our laboratory at the time of the study, but the two dimension microbiological agar well assay used (if well designed and performed) has been proved to be sensitive and in excellent agreement with other techniques (immunological, HPLC). [10][11][12] …”

Adsorptive Stripping Voltammetric Determination of Bupropion in Pharmaceuticals

“…As the scan rate increases gradually from the range 50 to 175 mV/s at fixed concentration of bupropion, the background signal decreases and peak potential shifted towards more negative value with increase in current confirming the irreversible nature of the reduction process [20][21][22][23][24] . Furthermore, peak current (Ip) found to be linear dependent on square root of scan rate related with the Randles -Servick equation, which can be expressed as:…”

High performance liquid chromatography method for the determination of meropenem in human plasma

“…Since the SPE method is not confined to low pH values, we avoid the limitation to inject the samples into the HPLC system within i hour after preparation, as recommended by A1-Meshal et al [3]. Although, the method proposed by A1-Meshal et al appears to be simple and less time consuming, it has to face the problem of meropenem deterioration in acidic media.…”

“…Meropenem has a methyl group at the position of the 5-membered ring, which confers stability against hydrolysis by dehydropeptidase i and therefore, does not require the addition of cilastatiin, as does imipenem. Deproteinization with TCA, although the most sensitive [3], has some serious limitations due to the instability of meropenem in low pH values, as discussed later. Several HPLC methods for the determination of meropenem in plasma are reported.…”