Determination of metal ions complexed with 2,6-diacetylpyridine bis(N-methylenepyridiniohydrazone) by capillary electrophoresis

Timerbaev, Andrei R.; Semenova, Olga; Bonn, Guenther K.; Fritz, James S.

doi:10.1016/0003-2670(94)80256-4

Cited by 38 publications

(11 citation statements)

References 17 publications

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“…However, the number of metals that can be separated in this mode is limited by a narrow migration window and/or by their similar mobilities. When using MEKC, the polarity can be reversed by an addition of a cationic surfactant to the electrolyte, and thus the separation can be improved [36]. To date, the conversion of metals into neutral complexes have been used only rarely [34,37].…”

Section: Uv/vis Detectionmentioning

confidence: 99%

Derivatization of inorganic ions in capillary electrophoresis

Padarauskas

2003

Electrophoresis

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This review gives a short overview of the main approaches to the derivatization of inorganic ions in capillary electrophoresis (CE) with emphasis on the most recent works. Various derivatization procedures and detection methods are discussed. A brief account of their advantages and limitations is given. More specific areas such as microchip CE, simultaneous separation of anions and cations, and speciation analysis are also briefly discussed.

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Section: Uv/vis Detectionmentioning

confidence: 99%

Derivatization of inorganic ions in capillary electrophoresis

Padarauskas

2003

Electrophoresis

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“…The metal ions had to be stabilized by complexing with a strong ligand, where the resulting complex had a positive charge, so that the metals could migrate towards the capillary outlet. The compound 2,6-diacetylpyridine-bis(N-methylenepyridiniohydrazone), referred to above, fulfilled these requirements [29] and was used to complex the metal ions. The mass flow MF std of the standard was calculated from the base-peak width t peak in Fig.…”

Section: Ce-icp-ms System For On-line Isotope Dilutionmentioning

confidence: 99%

Characterization and quantification of metallothionein isoforms by capillary electrophoresis–inductively coupled plasma–isotope-dilution mass spectrometry

et al. 2001

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In a new approach to the characterization and quantification of metallothionein isoforms an on-line isotope-dilution method in combination with the coupling of capillary electrophoresis (CE) to an inductively coupled plasma-sector field mass spectrometer (ICP-SFMS) is reported. Metallothionein (MT) isoforms are separated by CE and the elements Cu, Zn, Cd, and S are detected simultaneously by use of ICP-SFMS in the medium resolution mode. On-line isotope dilution is performed by continuous introduction of an isotopically enriched, species-unspecific spike solution after the separation step. MT from rabbit liver and a further purified MT-1 isoform were quantified by determination of sulfur, and the stoichiometric compositions of the metalloprotein complexes are characterized by determination of their sulfur-to-metal ratios.

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“…In the case of charged metal complexes, surfactants are often used as electrolyte additives with the purpose to exploit the separation principle of MEKC [34,36,44,45] but without evidence that micellar solubilization did take place. For example, changes in selectivity for metal complexes of trans-1,2-cyclohexanediaminetetraacetic acid (CDTA) were found to arise chiefly from differences in electrophoretic mobility, indicating insignificant partitioning of the highly charged complexes into the anionic SDS micelles [36].…”

Section: Charged Chelatesmentioning

confidence: 99%

Quantitative structure-mobility relationship modelling of electrokinetic chromatography of metal complexes: Approaches and limitations

2002

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A charged surfactant or an ionic polymer added to the capillary electrolyte introduces micellar solubilization and ion-exchange interactions, respectively, as a supplementary separation principle for metal complexes among a great many other analytes. Acting as a pseudostationary phase, such electrolyte additives make the separation mechanism fairly different from that based only on differences in electrophoretic mobility. A range of quantitative structure-mobility relationships were developed to explain the migration behavior of metal complexes in micellar and ion-exchange electrokinetic chromatographic systems as a function of their primary structural parameters. The validity of migration models tested using a comprehensive selection of experimental data available in the literature was shown to depend significantly on the judicious choosing of the analyte structural descriptors and on the dominance of either of the electrophoretic mobility of the analyte, partitioning into the micelle, and the ion-exchange interaction with the polymer. In the systems where the separation mechanism is dominated by neither chromatography nor electrophoresis, their relative contributions were accounted for by the degree of correlation between experimental and calculated mobilities and by variations in the absolute value and sign of regression coefficients at the most influencing parameters.

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Determination of metal ions complexed with 2,6-diacetylpyridine bis(N-methylenepyridiniohydrazone) by capillary electrophoresis

Cited by 38 publications

References 17 publications

Derivatization of inorganic ions in capillary electrophoresis

Derivatization of inorganic ions in capillary electrophoresis

Characterization and quantification of metallothionein isoforms by capillary electrophoresis–inductively coupled plasma–isotope-dilution mass spectrometry

Quantitative structure-mobility relationship modelling of electrokinetic chromatography of metal complexes: Approaches and limitations

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