Determination of Methylmalonic Acid by High-Performance Liquid Chromatography

Babidge, P.J.; Babidge, WJ

doi:10.1006/abio.1994.1062

Cited by 24 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We used a HPLC method, previously developed for veterinary biological samples [16]. We modified this method slightly to measure urinary MMA in human.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Urinary methylmalonic acid levels in patients with acute ischemic stroke

Kara

Şeneş

Coşkun

et al. 2009

Clinical Biochemistry

View full text Add to dashboard Cite

“…We used a HPLC method, previously developed for veterinary biological samples [16]. We modified this method slightly to measure urinary MMA in human.…”

Section: Discussionmentioning

confidence: 99%

“…The MMA method developed by Babidge and Babidge [16] was modified. MMA was extracted two times by ethylacetate, derivatized with monodansylcadaverine, separated by reverse phase HPLC and detected fluorometrically.…”

Section: Mma Measurementmentioning

confidence: 99%

Urinary methylmalonic acid levels in patients with acute ischemic stroke

Kara

Şeneş

Coşkun

et al. 2009

Clinical Biochemistry

View full text Add to dashboard Cite

“…The intra- and inter-CVs for this assay were 2.0% and 2.7%, respectively. Plasma MMA was analysed using HPLC [14]. The differences of duplicate analysis of 16 samples ranged from 0.0 to 10.9%, while the interassay CVs for five samples (concentration range: 177–1114 nmol/L) were between 6 and 12%.…”

Section: Methodsmentioning

confidence: 99%

Plasma and Red Cell Reference Intervals of 5-Methyltetrahydrofolate of Healthy Adults in Whom Biochemical Functional Deficiencies of Folate and Vitamin B₁₂Had Been Excluded

Sobczyńska‐Malefora

Harrington

Voong

et al. 2014

Advances in Hematology

View full text Add to dashboard Cite

5-Methyltetrahydrofolate (5-MTHF) is the predominant form of folate and a strong determinant of homocysteine concentrations. There is evidence that suboptimal 5-MTHF availability is a risk factor for cardiovascular disease independent of homocysteine. The analysis of folates remains challenging and is almost exclusively limited to the reporting of “total” folate rather than individual molecular forms. The purpose of this study was to establish the reference intervals of 5-MTHF in plasma and red cells of healthy adults who had been prescreened to exclude biochemical evidence of functional deficiency of folate and/or vitamin B12. Functional folate and vitamin B12 status was assessed by respective plasma measurements of homocysteine and methylmalonic acid in 144 healthy volunteers, aged 19–64 years. After the exclusion of 10 individuals, values for 134 subjects were used to establish the upper reference limits for homocysteine (13 μmol/L females and 15 μmol/L males) and methylmalonic acid (430 nmol/L). Subjects with values below these cutoffs were designated as folate and vitamin B12 replete and their plasma and red cell 5-MTHF reference intervals determined, N = 126: 6.6–39.9 nmol/L and 223–1041 nmol/L, respectively. The application of these intervals will assist in the evaluation of folate status and facilitate studies to evaluate the relationship of 5-MTHF to disease.

show abstract

“…Plasma concentrations of tHcy [14], MMA [15], phylloquinone [16,17] and red-cell 5MTHF were determined by high performance liquid chromatography (HPLC) with fluorescence detection; 5MTHF by an in-house method incorporating some published features [18,19]. Plasma folate/vitamin B 12 and red-cell folate were measured by radioassays (Quantaphase II, Bio-Rad, Hercules, California, USA).…”

Section: Patient Recruitmentmentioning

confidence: 99%

Erythrocyte folate and 5-methyltetrahydrofolate levels decline during 6 months of oral anticoagulation with warfarin

Sobczyńska‐Malefora

Harrington

Lomer

et al. 2009

Blood Coagulation &Amp; Fibrinolysis

View full text Add to dashboard Cite

Dietary fluctuations of vitamin K are detrimental to oral anticoagulant control. Attempts to improve control through the avoidance of vitamin K-rich foods (mainly green vegetables) may inadvertently compromise folate status, itself a risk factor for thromboembolism. We evaluated the effect of a 6-month period of warfarin therapy on folate status in 114 patients using measurements of red-cell folate and 5-methyltetrahydrofolate and plasma folate and total homocysteine. Circulatory levels of phylloquinone, vitamin B12 and methylmalonic acid were also determined. A subset of 45 patients completed 7-day food diaries at the beginning and end of their treatment. There was a significant decrease in total erythrocyte folate (P = 0.005) and 5-methyltetrahydrofolate (P = 0.002) during the study. A concurrent increase in plasma phylloquinone (P = 0.003) was attributed to warfarin-induced perturbation of vitamin K metabolism. No other longitudinal changes were observed. Folate and phylloquinone intakes correlated with each other at baseline (P = 0.024) and after treatment (P = 0.011). Based on robust measurements of erythrocyte folates, patients showed a significant impairment in folate status after 6-month therapy with warfarin. The majority of patients had intakes of folate and phylloquinone below the national average or UK guidelines. The study highlights the need for improved dietary management of patients taking oral anticoagulants.

show abstract

Determination of Methylmalonic Acid by High-Performance Liquid Chromatography

Cited by 24 publications

References 0 publications

Urinary methylmalonic acid levels in patients with acute ischemic stroke

Urinary methylmalonic acid levels in patients with acute ischemic stroke

Plasma and Red Cell Reference Intervals of 5-Methyltetrahydrofolate of Healthy Adults in Whom Biochemical Functional Deficiencies of Folate and Vitamin B₁₂Had Been Excluded

Erythrocyte folate and 5-methyltetrahydrofolate levels decline during 6 months of oral anticoagulation with warfarin

Contact Info

Product

Resources

About

Determination of Methylmalonic Acid by High-Performance Liquid Chromatography

Cited by 24 publications

References 0 publications

Urinary methylmalonic acid levels in patients with acute ischemic stroke

Urinary methylmalonic acid levels in patients with acute ischemic stroke

Plasma and Red Cell Reference Intervals of 5-Methyltetrahydrofolate of Healthy Adults in Whom Biochemical Functional Deficiencies of Folate and Vitamin B12Had Been Excluded

Erythrocyte folate and 5-methyltetrahydrofolate levels decline during 6 months of oral anticoagulation with warfarin

Contact Info

Product

Resources

About

Plasma and Red Cell Reference Intervals of 5-Methyltetrahydrofolate of Healthy Adults in Whom Biochemical Functional Deficiencies of Folate and Vitamin B₁₂Had Been Excluded