Determination of methylphenidate in plasma and saliva by liquid chromatography/tandem mass spectrometry

Seçilir, A.; Schrier, Lenneke; Bijleveld, Yuma A.; Toersche, Jan; Jorjani, Sona; Burggraaf, J.; Gerven, Joop van; Mathôt, Ron A. A.

doi:10.1016/j.jchromb.2013.01.027

Cited by 28 publications

(10 citation statements)

References 27 publications

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“…This is in agreement with the data of Josefsson and Rydberg . The instability reported by Seçilir et al was not observed in our experiments.…”

Section: Resultssupporting

confidence: 94%

“…Oral fluid for monitoring the exposure to methylphenidate is of great interest in the pediatric community as it is completely noninvasive and can take place on‐site. Compared to plasma, methylphenidate drug levels in oral fluid were found to be three‐ to tenfold higher . Thus, monitoring of methylphenidate in oral fluid caught our interest.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, an enantioselective CE‐based assay for determining saliva levels of methylphenidate was developed in our laboratory. As ppb drug levels have to be measured , head‐column field amplified sample stacking (FASS) was employed. This approach permits solutes to be injected electrophoretically into the capillary from a low‐conductivity sample and without introduction of a significant amount of sample solvent.…”

Section: Introductionmentioning

confidence: 99%

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Monitoring of threo‐methylphenidate enantiomers in oral fluid by capillary electrophoresis with head‐column field‐amplified sample injection

Theurillat

Thormann

2013

Electrophoresis

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Threo-methylphenidate is a chiral psychostimulant drug widely prescribed to treat attention-deficit hyperactivity disorder in children and adolescents. An enantioselective CE-based assay with head-column field-amplified sample stacking for analysis of threo-methylphenidate enantiomers in liquid/liquid extracts of oral fluid is described. Analytes are electrokinetically injected across a short water plug placed at the capillary inlet and become stacked at the interface between plug and buffer. Enantiomeric separation occurs within a few minutes in a pH 3.0 phosphate/triethanolamine buffer containing 20 mg/mL (2-hydroxypropyl)-β-CD as chiral selector. The assay with six point multilevel internal calibration provides a linear response for each enantiomer in the 10-200 ng/mL concentration range, is simple, inexpensive, and reproducible, and has an LOQ of 5 ng/mL. It was applied to oral fluid patient samples that were collected up to 12 h after intake of an immediate release tablet and two different extended release formulations with racemic methylphenidate. Drug profiles could thereby be assessed in a stereoselective way. Almost no levorotary threo-methylphenidate enantiomer was detected after intake of the two extended release formulations, whereas this enantiomer was detected during the first 2.5 h after intake of the immediate release preparation. The noninvasive collection of oral fluid is an attractive alternative to plasma for the monitoring of methylphenidate exposure in the pediatric community.

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“…This is in agreement with the data of Josefsson and Rydberg . The instability reported by Seçilir et al was not observed in our experiments.…”

Section: Resultssupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Monitoring of threo‐methylphenidate enantiomers in oral fluid by capillary electrophoresis with head‐column field‐amplified sample injection

Theurillat

Thormann

2013

Electrophoresis

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“…Various analytical methods such as immunoassay [13], HPLC with UV detection [14] and more recently liquid chromatographyelectrospray ionization mass spectrometry [15][16][17], have been proposed for measuring MPH (in serum).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Surface Molecularly Imprinting Polymers for Methylphenidate and its Application in Separating Methylphenidate

Khansari¹,

Shahreza²,

Rezvanirad³

et al. 2017

J Anal Bioanal Tech

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In this study, a novel approach is proposed for determination of methylphenidate in biological fluids. In this method molecularly imprinted solid-phase extraction (MISPE), as the sample extraction technique, combined with high-performance liquid chromatography (HPLC) is used. The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, Hexane as porogen and methylphenidate as template molecule. Extraction of methylphenidate from human serum was carried out using a novel imprinted polymer as the solid-phase extraction (SPE). Various parameters affecting the extraction efficiency of the polymer were evaluated. Also, the optimal conditions for the MIP cartridges were studied. The limit of detection (LOD) and limit of quantification (LOQ) for methylphenidate in serum samples were 1.3 and 10 ng mL -1 , respectively. The recoveries for serum samples were higher than 92%.

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“…Spectrophotometric, RP-HPLC and LCMS methods [2]- [8] gas-chromatographic-mass spectrometric procedures described for quantitation of methylphenidate and ritalinic acid [9]. Analytical method was developed and validated as per the ICH guidelines for the quantification of known and unknown impurities by HPLC.…”

Section: Introductionmentioning

confidence: 99%

Drug-Excipient Interaction of Methylphenidate with Glycerin in Methylphenidate Oral Solution and Identification of its Transesterification Products by UPLC-MS/MS

Hotha¹,

Roychowdhury²,

Subramanian³

2016

AJAC

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Reactions between active drug substances and excipients are of interest in the drug formulation process should be checked for the interactions during the storage conditions. Some excipients react with certain chemical groups in drug substances which will form new impurities in the finished product formulations. In the present paper transesterification reaction of methylphenidate with glycerin to form different structural isomeric products was described. These impurities identified in forced degradation studies, excipient compatibility studies and stability analysis of the finished product. Stability samples were analyzed and observed that about ~0.6% of the Methylphenidate content was transformed into methylphenidate-glycerin isomers within 3 Months at 40˚C/75% RH and 18 Months at 25˚C/60% RH conditions. Analysis of two lots of marketed preparations having expiry dates in 2012 and 2013 showed content of the Methylphenidate esters corresponding to ~0.6% of the declared Methylphenidate content. The samples of this impurity were investigated by HPLC, UPLC-MS/MS to generate the mechanism of the impurity formation.

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Determination of methylphenidate in plasma and saliva by liquid chromatography/tandem mass spectrometry

Cited by 28 publications

References 27 publications

Monitoring of threo‐methylphenidate enantiomers in oral fluid by capillary electrophoresis with head‐column field‐amplified sample injection

Monitoring of threo‐methylphenidate enantiomers in oral fluid by capillary electrophoresis with head‐column field‐amplified sample injection

Synthesis of Surface Molecularly Imprinting Polymers for Methylphenidate and its Application in Separating Methylphenidate

Drug-Excipient Interaction of Methylphenidate with Glycerin in Methylphenidate Oral Solution and Identification of its Transesterification Products by UPLC-MS/MS

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