Determination of mevalonic acid in human urine by liquid chromatography with precolumn derivatization and fluorescence detection

On-Line Coupling of Solid Phase Extraction Sample Processing with High-Performance Liquid Chromatography

“…The sample is then purified on a SPE precolumn and finally transferred by the mobile phase to the analytical column. [37][38][39][40][41] Other assays are based on the use of reagents attached to solid supports. 42,43 These materials effect sample clean-up and at the same time, or somewhat later, the reagent part of material is made to react with the analyte.…”

“…Immunosorbents and molecular imprinted materials, which are based on molecular recognition, also allow achieving isolation and preconcentration in a single step. On-line SPE sample processing is very well suited for analysis of biological fluids such as urine [7][8][9][10]15,[20][21][22][23][25][26][27]30,31,37,39,[44][45][46]59,[94][95][96][97][98][99]102 and plasma. 7,8,15,16,18,20,22,33,36,37,56,57,69,[70][71][72][73]94,96,100 The determination of various pharmaceutics and drugs of this kind of matrices is required for pharmacokinetic and bioavailability studies 7,8,15,16, 30,38, 45,56, …”

The validation of a simple LC/MS/MS method for determining the level of mevalonic acid in human plasma

“…1), a fundamental intermediate in the cholesterol biosynthetic pathway (Bjorkhem et al ., 1987; Krause and Newton, 1995). Plasma and urine concentrations of mevalonic acid (MVA) have been shown to correlate with the biosynthetic rate and long‐term changes of cholesterol levels in preclinical models and humans (Popjak et al ., 1979; Kawasaki et al ., 1998; Saisho et al ., 1997). Therefore, determining the concentration of MVA in these accessible (peripheral) biological fluids can be used as a mechanistic biomarker of HMG‐CoA reductase inhibition localized in a sequestered target space (e.g.…”