Determination of monosaccharides in Lycium barbarum fruit polysaccharide by an efficient UHPLC‐QTRAP‐MS/MS method

Abstract

Introduction Pre‐column derivatisation using 1‐phenyl‐3‐methyl‐5‐pyrazolone (PMP) is a common method for monosaccharide determination. Herein, a specific ultra‐high‐performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC‐QTRAP‐MS/MS) method was developed and used for the determination of monosaccharide composition of Lycium barbarum polysaccharide (LBP). Objective Exploration of a new efficient method for monosaccharide determination. Methods In our study, hydrochloric acid was used to hydrolyse the polysaccharide obtained from L. barbarum fruits. Principal component analysis (PCA) and technique for order of preference by similarity to ideal solution (TOPSIS) analysis were used for comprehensive evaluation of the samples. The results showed that LBP was composed of seven monosaccharides: galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose. The linear relationship of the seven monosaccharides was optimum within a certain concentration range. Quantitative recoveries of the seven monosaccharides from the samples ranged from 94.76% to 102.11%. Results A rapid quantitative detection method was established, in which the hydrolysis time was reduced from 12 h to 2 h. By using LBP as one of the indexes to evaluate the quality of L. barbarum, L. barbarum from Zhongning County, Ningxia Province, was identified as having the best quality among the varieties tested. Conclusion The UHPLC‐QTRAP‐MS/MS pre‐column derivatisation method used in this study was simple, accurate, and sensitive, with good repeatability, and can be used for quality evaluation and origin distinction of L. barbarum and other medicinal plants.

Effects of Lycium barbarum L. Polysaccharides on Vascular Retinopathy: An Insight Review

“…The results of ultra-high-performance liquid chromatography quadrupole trap tandem mass spectrometry (UHPLC-QTRAP-MS/MS) showed that the LBP was composed of galactose, arabinose, mannose, rhamnose, xylose, ribose, and glucose, among which glucose was the most abundant monosaccharide [ 25 ]. As shown in Figure 1 , (1→3) -β-D-pyranogalactosyl, (1→4) -α-D-pyranogalactosyl residues, and (1→6) -β-D-pyranogalactosyl are three common characteristic structures [ 24 ].…”

Application of ligninolytic bacteria to the enhancement of lignocellulose breakdown and methane production from oil palm empty fruit bunches (OPEFB)

“…An UHPLC system (Agilent infinity 1290, Agilent technologies) with a triple quadrupole-linear tandem with ion trap Mass Spectrometry detector (LC-MS/MS-API 4000 QTRAP from AB-Sciex) was used to analyse the sugar component (glucose and xylose). The method used was modified from Xu et al (2021). The liquid extract samples from non-treated and treated samples was evaporated at a temperature of 40C until dry and diluted using acetonitrile and water (ACN:water) 50:50, followed by homogenisation using vortex and sonicator.…”

Complete composition analysis of polysaccharides based on HPAEC‐PAD coupled with quantitative analysis of multi‐components by single marker

“…In recent years, polysaccharides have attracted much attention because of their complex structure and diverse biological activities, including antioxidant, antitumour, hepatoprotective, hypoglycemic, anti-bacterial, antiinflammatory and immunological activities. [2][3][4][5][6][7][8][9][10] Currently, the challenges of monosaccharide composition analysis (MCA) of polysaccharides are mainly reflected in the following aspects: (1) the complexity of polysaccharides makes it difficult to liberate monosaccharides completely; (2) inappropriate hydrolysis conditions may result in the degradation of released monosaccharides;…”

“…They widely exist in plants, animals, and microorganisms. In recent years, polysaccharides have attracted much attention because of their complex structure and diverse biological activities, including antioxidant, antitumour, hepatoprotective, hypoglycemic, anti‐bacterial, anti‐inflammatory and immunological activities 2–10 …”