Determination of N-acetyl-DL-leucine in the saliva of healthy volunteers and diabetic patients using ultra-performance liquid chromatography with fluorescence detection

Qi, Chao; Li, Wenhao; Shi, Qing; Zhao, Lili; Jin, Wenlong; Min, Jun Zhe

doi:10.1016/j.cca.2021.12.014

Cited by 7 publications

(2 citation statements)

References 44 publications

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“…NMR-based studies have reported higher concentrations of acetylated small molecules in healthy individuals compared to patients with chronic periodontitis. 31 , 32 , 33 Moreover, N-acetyl-leucine levels were found to be significantly lower in the saliva of patients with diabetic, 34 and acetylated metabolite levels were proposed to discriminate between oral squamous cell carcinoma and oral lichen planus. 35 To this end, the origin and the functional role of N-acetylated metabolites in the oral cavity are unknown.…”

Section: Discussionmentioning

confidence: 98%

Spatially and temporally resolved metabolome of the human oral cavity

Ciurli,

Mohammed,

Ammon

et al. 2024

iScience

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Section: Discussionmentioning

confidence: 98%

Spatially and temporally resolved metabolome of the human oral cavity

Ciurli,

Mohammed,

Ammon

et al. 2024

iScience

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“…It is used to treat neurodegenerative diseases [39]. Recently, it was described that N-acetyl-L-leucine levels are increased in hair and saliva of Type 2 diabetes patients [40,41]. Hexanoylglycine is an acyl glycine, a unusual minor metabolite of fatty acid resulting from glycine acyltransferase action, an important enzyme for xenobiotic detoxification, especially of benzoate and hydroxybenzoates [42].…”

Section: Blood Serum Metabolome Changes On Active Tuberculosismentioning

confidence: 99%

Mass Spectrometry-Based Proteomic and Metabolomic Profiling of Serum Samples for Discovery and Validation of Tuberculosis Diagnostic Biomarker Signature

Fernandes

Gonçalves

Bento

et al. 2022

IJMS

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Tuberculosis (TB) is a transmissible disease listed as one of the 10 leading causes of death worldwide (10 million infected in 2019). A swift and precise diagnosis is essential to forestall its transmission, for which the discovery of effective diagnostic biomarkers is crucial. In this study, we aimed to discover molecular biomarkers for the early diagnosis of tuberculosis. Two independent cohorts comprising 29 and 34 subjects were assayed by proteomics, and 49 were included for metabolomic analysis. All subjects were arranged into three experimental groups—healthy controls (controls), latent TB infection (LTBI), and TB patients. LC-MS/MS blood serum protein and metabolite levels were submitted to univariate, multivariate, and ROC analysis. From the 149 proteins quantified in the discovery set, 25 were found to be differentially abundant between controls and TB patients. The AUC, specificity, and sensitivity, determined by ROC statistical analysis of the model composed of four of these proteins considering both proteomic sets, were 0.96, 93%, and 91%, respectively. The five metabolites (9-methyluric acid, indole-3-lactic acid, trans-3-indoleacrylic acid, hexanoylglycine, and N-acetyl-L-leucine) that better discriminate the control and TB patient groups (VIP > 1.75) from a total of 92 metabolites quantified in both ionization modes were submitted to ROC analysis. An AUC = 1 was determined, with all samples being correctly assigned to the respective experimental group. An integrated ROC analysis enrolling one protein and four metabolites was also performed for the common control and TB patients in the proteomic and metabolomic groups. This combined signature correctly assigned the 12 controls and 12 patients used only for prediction (AUC = 1, specificity = 100%, and sensitivity = 100%). This multiomics approach revealed a biomarker signature for tuberculosis diagnosis that could be potentially used for developing a point-of-care diagnosis clinical test.

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Development of a novel method for analysing N-acetyl-DL-leucine enantiomers in human fingernail by UPLC-ESI-MS/MS and the evaluation in diabetes mellitus

Zhou

Takashi

et al. 2023

Clinica Chimica Acta

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Determination of N-acetyl-DL-leucine in the saliva of healthy volunteers and diabetic patients using ultra-performance liquid chromatography with fluorescence detection

Cited by 7 publications

References 44 publications

Spatially and temporally resolved metabolome of the human oral cavity

Spatially and temporally resolved metabolome of the human oral cavity

Mass Spectrometry-Based Proteomic and Metabolomic Profiling of Serum Samples for Discovery and Validation of Tuberculosis Diagnostic Biomarker Signature

Development of a novel method for analysing N-acetyl-DL-leucine enantiomers in human fingernail by UPLC-ESI-MS/MS and the evaluation in diabetes mellitus

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