Determination of neomycin and related substances in pharmaceutical preparations by reversed-phase high performance liquid chromatography with mass spectrometry and charged aerosol detection

Photochemical Transformation of Aminoglycoside Antibiotics in Simulated Natural Waters

“…Control experiments showed that the direct photodegradation of aminoglycosides at pH 7.0 in ultrapure water was negligible, as illustrated in Figure . This was due to the lack of UV–vis absorption of aminoglycosides within the solar irradiation spectrum. , The photodegradation of aminoglycosides in the presence of SRNOM was observed, which suggested that photosensitized degradation is critical in the environmental phototransformation of aminoglycosides. The indirect photodegradation followed pseudo-first-order kinetics, and the rate constants of kanamycin and streptomycin were found to be 0.226 and 0.221 h –1 , respectively.…”

Validation of the high performance liquid chromatography method for the analysis of neomycin sulfate with resonance Rayleigh scattering detection

“…13 NM cannot be determined using direct UV or uorescence detection because of the lack of a suitable UV absorbing or uorescence chromophore. 14 Various methods have been employed to determine neomycin sulfate, including pulsed electrochemical detection (PED), 15 evaporative light scattering detection (ELSD), 16 charged aerosol detection (CAD), 17 and reversed phase chromatography and tandem mass spectrometry (MS/MS) detection (HPLC-MS/MS). 18 In the case of the aminoglycosides, because there are several amino groups in these compounds, multiple derivatives methods have mainly used pre-or post-column derivatization, such as the derivative reaction with o-phthalaldehyde (OPA), 19 phthalimidylbenzoyl chloride (Phibyl-Cl) 20 and 3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride (DMEQ-Cl).…”

Corona charged aerosol detector non-uniform response factors of purified alcohol ethoxylated homologues using liquid chromatography

“…Stypulkowska et al consolidated this trend with two different compounds: neamine and framycetin sulfate. Response factors calculated were 0.91 and 1.1 respectively [45].…”