Determination of nicorandil concentrations in human plasma using liquid chromatography

Ojha, Ashwini; Pargal, Anisha

doi:10.1016/s0731-7085(99)00102-8

Cited by 17 publications

(7 citation statements)

References 6 publications

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“…Besides the low limit of quantitation (5 ng.ml À1 ), the developed method exhibits a major advantage compared with most of the assays for nicorandil, [10][11][12][13][14] when considering its short run time. The previous papers present total run time higher than 8 min; while using our developed method, the retention times of nicorandil, NHN and IS were 4.9 min, 2.4 min and 1.9 min, respectively (Fig.…”

Section: Conditions For Lc-esi-ms/msmentioning

confidence: 99%

“…Some methods have been reported for quantitating nicorandil in plasma, using high performance liquid chromatography (HPLC) coupled to ultraviolet (UV) detection . Due to the low sensitivity and selectivity of UV detection, mass spectrometry (MS) was employed in some studies .…”

Section: Introductionmentioning

confidence: 99%

“…Some methods have been reported for quantitating nicorandil in plasma, using high performance liquid chromatography (HPLC) coupled to ultraviolet (UV) detection. [10][11][12][13] Due to the low sensitivity and selectivity of UV detection, mass spectrometry (MS) was employed in some studies. [14,15] However, as far as we know, no analytical method has been reported for the simultaneous quantitation of nicorandil and its major active metabolite, NHN, in plasma, using LC-ESI-MS/MS.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous quantitation of nicorandil and its denitrated metabolite in plasma by LC‐MS/MS: application for a pharmacokinetic study

et al. 2011

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A liquid chromatography-electrospray ionization tandem mass spectrometry method was developed and validated for the simultaneous quantitation of nicorandil and its denitrated metabolite, N-(2-hydroxyethyl)-nicotinamide, in rat plasma. After a liquid-liquid extraction step, chromatographic separation was performed on a ShinPack C(18) column with an isocratic mobile phase composed of methanol and 2 mM aqueous ammonium acetate containing 0.03% (v/v) formic acid (33:67 v/v). Procainamide was used as an internal standard (IS). Selected reaction monitoring was performed using the transitions m/z 212 → m/z 135, m/z 166 → m/z 106 and m/z 236 → m/z 163 to quantify nicorandil, its denitrated metabolite and IS, respectively. Calibration curves were constructed over the range of 5-15,000 ng.ml(-1) for both nicorandil and its metabolite. The mean relative standard deviation (RSD%) values for the intra-run precision were 5.4% and 7.3% and for the inter-run precision were 8.5% and 7.3% for nicorandil and its metabolite, respectively. The mean accuracy values were 100% and 95% for nicorandil and its metabolite, respectively. No matrix effect was detected in the samples. The validated method was successfully applied to a pharmacokinetic study after per os administration of nicorandil in rats.

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Section: Conditions For Lc-esi-ms/msmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simultaneous quantitation of nicorandil and its denitrated metabolite in plasma by LC‐MS/MS: application for a pharmacokinetic study

et al. 2011

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“…Various kind of methods for determining nicorandil in human plasma have been reported (Andrensek et al, 1999;Bachert and Fung, 1993;Mawatari et al, 1996;Ojha and Pargal, 1999). These methods are HPLC method using fluorescence detector (Mawatari et al, 1996) and liquid chromatographic method (Ojha and Pargal, 2003).…”

Section: Introductionmentioning

confidence: 99%

HPLC Method for the Determination of Nicorandil in Human Plasma

Shin¹

2008

Biomolecules and Therapeutics

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− The present study is to determine of sensitive nicorandil analysis method using HPLC and measure the pharmacokinetics parameters (bioavailability, C max , T max , Ke, T 1/2 ) of nicorandil (5 mg, Tab; Choongwae Pharma Corporation). Plasma (500 ul) was mixed with furosemide (internal standard, 500 ug/ml). Detection wavelength was 256 nm. The mixture of 0.01 M ammonium acetate and acetonitrile 80:20 (v/v) was used mobile phase. The HPLC separation was accomplished on ODC reverse HPLC column. The nicorandil was analyzed by a HPLC system, which consists of CAPCELL PAK C18 column (5 µm, 4.6 × 150 mm) and a chromatography data analysis S/W, using a isocratic mobile phase (mixture of 0.01 M ammonium acetate and acetonitrile 80:20 ) at 1.0 ml/min. Its sensitivity, selectivity, accuracy and precision must be adequate for the bioavailabilty study of nicorandil, and the linearity (r 2 ≥ 0.9994) of nicorandil was also proved in the range of 0.05 ug/ml − 3 ug/ml. The pharmacokinetic parameters of nicorandil (5 mg) tablets were measured as the follow. AUC: 0.19 ug/ml·hr, C max : 0.14 ug/ml, t max : 0.58 hr, Ke: 0.11 hr − , t 1/2β : 6.76 hrs. This method is simple and sensitive HPLC method using UV detector for determination of nicorandil in human plasma.

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“…2) Many eŠorts have been made to determine nicorandil concentration in biological ‰uids and drug formulations. The techniques used for the assay include high performance liquid chromatography, [3][4][5][6][7][8] high performance thin layer chromatography, 9) gas chromatography coupled with mass spectrometry, 10) and polarography. 11) The literature reveals that few spectrophotometric methods have also been developed to determine nicorandil in drug formulations and biological ‰uids.…”

Section: Introductionmentioning

confidence: 99%

Quantitation of Nicorandil in Pharmaceutical Formulations by Spectrophotometry Using N-(1-naphthyl) Ethylenediamine Dihydrochloride as Coupling Agent

2007

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A validated and sensitive spectrophotometric method is developed for the quantitation of nicorandil in pharmaceutical formulations. The method is based on the reduction of nitroxy ethyl group of nicorandil to carbonyl derivative and nitrite ion by Zn/NH 4 Cl. The nitrite ion undergoes diazotization with sulphanilamide in presence of HCl followed by coupling with N (1 naphthyl) ethylenediamine dihydrochloride (NED) to form a colored product with l max at 525 nm. Under the optimized experimental condition, Beer's law is obeyed in the concentration range of 0.4-12.0 mg/ml with molar absorptivity of 1.92×10 4 l mol -1 cm -1 . The statistical analysis of calibration data yields the linear regression equation: A＝6.304×10 -4 ＋9.13×10 -2 C with correlation coe‹cient of 0.9999. The limits of detection and quantiˆcation are 0.05 and 0.15 mg/ml, respectively. The results obtained by the proposed method are acceptable with average recoveries of 100.0-100.1％. The results of the proposed method are compared with those of the reference method by point and interval hypothesis tests, which showed excellent agreement and there is no signiˆcant diŠerence in accuracy and precision of methods compared.

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Determination of nicorandil concentrations in human plasma using liquid chromatography

Cited by 17 publications

References 6 publications

Simultaneous quantitation of nicorandil and its denitrated metabolite in plasma by LC‐MS/MS: application for a pharmacokinetic study

Simultaneous quantitation of nicorandil and its denitrated metabolite in plasma by LC‐MS/MS: application for a pharmacokinetic study

HPLC Method for the Determination of Nicorandil in Human Plasma

Quantitation of Nicorandil in Pharmaceutical Formulations by Spectrophotometry Using N-(1-naphthyl) Ethylenediamine Dihydrochloride as Coupling Agent

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