Determination of Nitric Oxide–Donor Effects on Tissue Gene Expression In Vivo Using Low‐Density Gene Arrays

Tran, Sorrel; Brazeau, Daniel A.; Fung, Ho‐Leung

doi:10.1016/s0076-6879(05)96033-3

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…TaqMan Arrays (TAs) have recently been introduced as a novel approach to measure gene expression. They combine the high sensitivity provided by the real time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) with the ability to simultaneously assay RNA expression levels of up to 384 target genes in a single sample [ 1 , 2 ]. However, an accurate quantification with this approach could be limited by the low sample amount commonly encountered in clinical research.…”

Section: Introductionmentioning

confidence: 99%

Multiplex preamplification of specific cDNA targets prior to gene expression analysis by TaqMan Arrays

et al. 2008

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Background: An accurate gene expression quantification using TaqMan Arrays (TA) could be limited by the low RNA quantity obtained from some clinical samples. The novel cDNA preamplification system, the TaqMan PreAmp Master Mix kit (TPAMMK), enables a multiplex preamplification of cDNA targets and therefore, could provide a sufficient amount of specific amplicons for their posterior analysis on TA.

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Section: Introductionmentioning

confidence: 99%

Multiplex preamplification of specific cDNA targets prior to gene expression analysis by TaqMan Arrays

et al. 2008

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“…If a twofold change was used as a threshold criterion, the results would indicate alteration in the expression of 65 and 23 genes, respectively, in mouse liver and lungs. Using more rigorous statistical approaches (Tran et al 2005), we found six specific genes to be significantly up-regulated, viz., those encoding VEGF and VEGFD in the lung, and FGF1, FGF4 (fibroblast growth factor 1 and 4, respectively), Hsp70 (heat shock 70-kDa protein 4), and PF4 (platelet factor 4) in the liver. Marginally stimulated genes in the liver were those encoding HO-1 (heme oxygenase-1) and Smad7.…”

Section: In Vivo Regulation Of Vegf Gene and Protein Expression By Inmentioning

confidence: 99%

Effects of Inhalant Nitrites on VEGF Expression: A Feasible Link to Kaposi's Sarcoma?

Fung

Tran

2006

Jrnl Neuroimmune Pharm

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Because inhalant nitrites (commonly known as "poppers") were thought to be rapidly cleared from the body, the lay literature has somewhat downplayed their toxicity. However, scientific reports have documented their immunosuppressive effects in animals, and epidemiological studies have implicated their use with the development of Kaposi's sarcoma (KS) in humans. Because inhalant nitrites are exogenous nitric oxide donors, we hypothesized that these substances of abuse might exert part of their toxicological effects through this biochemical product, which has been shown to alter gene regulation and angiogenesis. In a series of studies, we showed that acute and chronic in vivo exposure to isobutyl nitrite (a representative inhalant nitrite) produced significant tissue-dependent alterations in the expression of a number of cancer- and angiogenesis-related genes in mice. In particular, hepatic mRNA and protein expression of vascular endothelial growth factor (VEGF) was significantly stimulated. The in vivo growth rate of a subcutaneous VEGF-responsive tumor was also shown to be accelerated by inhalant nitrite exposure. Because the development of KS is extensively linked to VEGF and its receptors, the purported link between inhalant nitrites and KS may be explained mechanistically, at least in part, through the stimulation of VEGF expression by these inhalants.

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Effects of repeated in vivo inhalant nitrite exposure on gene expression in mouse liver and lungs

et al. 2006

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Determination of Nitric Oxide–Donor Effects on Tissue Gene Expression In Vivo Using Low‐Density Gene Arrays

Cited by 3 publications

References 15 publications

Multiplex preamplification of specific cDNA targets prior to gene expression analysis by TaqMan Arrays

Multiplex preamplification of specific cDNA targets prior to gene expression analysis by TaqMan Arrays

Effects of Inhalant Nitrites on VEGF Expression: A Feasible Link to Kaposi's Sarcoma?

Effects of repeated in vivo inhalant nitrite exposure on gene expression in mouse liver and lungs

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