Determination of Nitric Oxide in Biological Samples

Yao, Dachun; Vlessidis, Athanasios G.; Evmiridis, Nicholaos P.

doi:10.1007/s00604-004-0212-8

Cited by 47 publications

(59 citation statements)

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“…Diaminofluoresceins (DAFs) are markers used for detecting NO in tissues by fluorescence emission (Yao et al, 2004;Kojima et al, 1998). The chemical transformation of 4,5-diaminofluorescein compound (DAF-2) is based on the reactivity of aromatic diamines with NO in the presence of O2.…”

Section: Extracellular and Intracellular No Detectionmentioning

confidence: 99%

Evidence towards the involvement of nitric oxide in drought tolerance of sugarcane

Silveira

Hancock

Frungillo

et al. 2017

Plant Physiology and Biochemistry

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Exogenous supply of nitric oxide (NO) increases drought tolerance in sugarcane plants. However, little is known about the role of NO produced by plants under water deficit. The aim of this study was to test the hypothesis that drought-tolerance in sugarcane is associated with NO production and metabolism, with the more drought-tolerant genotype presenting higher NO accumulation in plant tissues. The sugarcane genotypes IACSP95-5000 (drought-tolerant) and IACSP97-7065 (drought-sensitive) were submitted to water deficit by adding polyethylene glycol (PEG-8000) in nutrient solution to reduce the osmotic potential to -0.4 MPa. To evaluate short-time responses to water deficit, leaf and root samples were taken after 24 h under water deficit. The drought-tolerant genotype presented higher root extracellular NO content, which was accompanied by higher root nitrate reductase (NR) activity as compared to the drought-sensitive genotype under water deficit. In addition, the drought-tolerant genotype had higher leaf intracellular NO content than the drought-sensitive one. IACSP95-5000 exhibited decreases in root S-nitrosoglutathione reductase (GSNOR) activity under water deficit, suggesting that S-nitrosoglutathione (GSNO) is less degraded and that the drought-tolerant genotype has a higher natural reservoir of NO than the drought-sensitive one. Those differences in intracellular and extracellular NO contents and enzymatic activities were associated with higher leaf hydration in the drought-tolerant genotype as compared to the sensitive one under water deficit.

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Section: Extracellular and Intracellular No Detectionmentioning

confidence: 99%

Evidence towards the involvement of nitric oxide in drought tolerance of sugarcane

Silveira

Hancock

Frungillo

et al. 2017

Plant Physiology and Biochemistry

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“…Optical and electrochemical methods are two main techniques for the determination of NO, which have been reviewed with respect to applications both in vivo or in vitro [17][18][19]. Electrochemical sensor represents one of the most promising approaches for the determination of NO for its low detection limit, fast response, easy manufacturing, small size, and allowing real-time measurement of NO concentration in biological samples [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Nanomaterials-based electrochemical sensors for nitric oxide

Dang

Wang

et al. 2014

Microchim Acta

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Electrochemical sensing has been demonstrated to represent an efficient way to quantify nitric oxide (NO) in challenging physiological environments. A sensing interface based on nanomaterials opens up new opportunities and broader prospects for electrochemical NO sensors. This review (with 141 refs.) gives a general view of recent advances in the development of electrochemical sensors based on nanomaterials. It is subdivided into sections on (i) carbon derived nanomaterials (such as carbon nanotubes, graphenes, fullerenes), (ii) metal nanoparticles (including gold, platinum and other metallic nanoparticles); (iii) semiconductor metal oxide nanomaterials (including the oxides of titanium, aluminum, iron, and ruthenium); and finally (iv) nanocomposites (such as those formed from carbon nanomaterials with nanoparticles of gold, platinum, NiO or TiO 2 ). The various strategies are discussed, and the advances of using nanomaterials and the trends in NO sensor technology are outlooked in the final section.

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“…Indirect detection of NO production by Griess reaction [17]. RAW264.7 cell was pipetted into a 96-well plate (100 l/well), each group treatment with corresponding LPS or BCP for 24 h. 50 l cell culture solution was taken from each well and tested with Griess reagent.…”

Section: Determination the Level Of Nitric Oxide (No)mentioning

confidence: 99%

Extraction, characterization and bioactivities of novel purified polysaccharides from Baphicacanthis Cusiae Rhizoma et Radix

Wang

Chen

et al. 2016

International Journal of Biological Macromolecules

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The purpose of this study was to investigate the extraction, characterization and bioactivities of purified water-soluble polysaccharides (BCP) from Baphicacanthis Cusiae Rhizoma et Radix. Based on the response surface methodology, the optimal extraction parameters were obtained as follows: extraction temperature of 60.0°C, extraction time of 35.0min, and ratio of water to raw material of 24.5ml/g. Then, BCP was separated and purified by chromatography of DEAE-52 and Sephadex G-100, and obtained two purified fractions, named as BCP-1 and BCP-2. Their molecular weights were respectively 11.6 and 26.7 KDa with mainly composed of glucose, arabinose and galactose. BCP-2 had higher contents of sulfuric radical and uronic acid than BCP-1. Finally, their antioxidant and anti-inflammatory activities were evaluated. Both of BCP-1 and BCP-2 exhibited strong antioxidant activity in vitro, and the antioxidant of BCP-2 was better. Besides, they showed ideal anti-inflammatory activity in vitro and in vivo.

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Determination of Nitric Oxide in Biological Samples

Cited by 47 publications

References 0 publications

Evidence towards the involvement of nitric oxide in drought tolerance of sugarcane

Evidence towards the involvement of nitric oxide in drought tolerance of sugarcane

Nanomaterials-based electrochemical sensors for nitric oxide

Extraction, characterization and bioactivities of novel purified polysaccharides from Baphicacanthis Cusiae Rhizoma et Radix

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