Determination of Novel Highly Effective Necrostatin Nec-1s in Rat Plasma by High Performance Liquid Chromatography Hyphenated with Quadrupole-Time-of-Flight Mass Spectrometry

Mikuš, Peter; Pecher, Daniel; Rauová, Drahomíra; Horváth, Csaba; Szobi, Adrián; Adameová, Adriana

doi:10.3390/molecules23081946

Cited by 7 publications

(1 citation statement)

References 16 publications

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“…according to previous results, serum SirT1 is highly sensitive and specific to the diagnosis of NEC, which is of certain reference value for the early diagnosis of nec (3). in a rat model, the results indicated that the expression level of SIRT1 was markedly decreased in the intestine of rats with nec induced by lPS, but this effect was reversed with SirT1 activator treatment to protect the intestine by inhibiting the inflammatory response (3,4). Therefore, SIRT1 may be a novel diagnostic biomarker of NEC and serves an important role in the development of nec.…”

Section: Introductionsupporting

confidence: 67%

miR‑34a increases inflammation and oxidative stress levels in patients with necrotizing enterocolitis by downregulating SIRT1 expression

Zhu

Liu

2021

Mol Med Rep

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The mir-34a/SirT1 signaling axis is an important signaling axis in tumors and diseases. notably, low SirT1 expression in the intestinal tissues of patients with necrotizing enterocolitis (nec) has been reported. However, whether mir-34a/SirT1 signaling as a target to protect the intestines during the nec process is unclear and remains to be elucidated. Blood samples were collected from 30 patients with nec, and an nec rat model was used. The mir-34a and SirT1 gene and protein expression levels were assayed by qPcr and Western blotting method. The inflammatory cytokine levels and oxidative stress levels were detected using the eliSa method. The results demonstrated that birth weight, albumin and glucose concentrations were significantly decreased in the NEC patient group compared with the control group, but the c-reactive protein (crP) and procalcitonin (PcT) concentrations were significantly increased. The mir-34a expression level was notably increased in the nec group, but the SirT1 expression level was markedly decreased. Notably, the miR-34a was significantly correlated with NEC severity and the concentrations of crP, PcT, il-6, TnF-α, il-1β, il-8, McP-1, VcaM1 and malondialdehyde (MDA), but was significantly negatively correlated with SirT1 gene expression and the concentration of il-10. intestinal villi damage in nec rats was decreased with mir-34a inhibition and SirT1 activation treatment by decreasing the levels of inflammatory cytokines, including il-6, TnF-α, il-1β and il-8, and oxidative stress proteins, including McP-1, VcaM1, and Mda, as well as increasing the level of the anti-inflammatory cytokine IL-10. In addition, the results indicated that mir-34a inhibition and SirT1 activation strongly protected the intestine and decreased the damage caused by nec, not only by decreasing the protein levels of SirT1, , but also by increasing the il-10 protein levels. The mir-34a inhibition and SirT1 activation may decrease the damage caused by nec by decreasing proinflammatory cytokines and oxidative stress proteins and by increasing the anti-inflammatory cytokine pathway. Based on the aforementioned analysis, the mir-34a and SirT1 proteins may be potential novel therapeutic targets in nec.

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Section: Introductionsupporting

confidence: 67%