Determination of ochratoxin�A in pig tissues by liquid?liquid extraction and clean-up and high-performance liquid chromatography

Abstract

A fast, simple, and sensitive HPLC-FD method is described for determination of ochratoxin A (OTA) in pig kidney and muscle; a small mass (<2.5 g) of sample and a relatively small volume (<15 mL) of a non-halogenated extraction solvent are required. Ochratoxin B, systematically absent from all the samples investigated, was used as internal standard. Liquid-liquid partition was used for sample clean-up. Recoveries at the 1 ng x g(-1) level were 86+/-15% and 74+/-8% for kidney and muscle, respectively, and detection limits were 0.14 and 0.15 ng x g(-1). Clean-up by solid-phase extraction (SPE) is required for pig liver. A survey of the OTA content of tissues of pigs slaughtered in southern Italy revealed that 52 out of 54 analysed samples were contaminated; the OTA concentration in kidney ranged between 0.26 and 3.05 ng x g(-1). The effect of measurement precision on compliance with legal limits is also discussed.

Quantitative determination of ochratoxin A in kidneys by liquid chromatography/mass spectrometry

“…ethyl acetate, coextracted polar interfering substances from the matrix to a greater extent. 11 However, by restriction of the sample size to 7.5 g in our method, less chloroform could be used than in previously described methods.…”

Detection and quantification of Ochratoxin A in milk produced in organic farms

“…In animal products, such as poultry and pork meat, OTA can be present both because of direct moulds contamination and because of a carry-over from contaminated feed stuffs Monaci, Tantillo, & Palmisano, 2004;Valenta, Khun, & Rohr, 1993;Valenta et al, 1993). Traces of OTA have been also found in human milk (Mantle, 2002;Turconi et al, 2004;Mantle, 2002, Turconi et al, 2004.…”

Estimation of ochratoxin A in portuguese population: New data on the occurrence in human urine by high performance liquid chromatography with fluorescence detection

“…As to food safety, the problem of OTA lies in the fact that its presence in human body is due to the ingestion of small quantities present in several commodities, such as, coffee, cereals, rice, dried fruits, beer and wine (Visconti et al, 2000;Blesa et al, 2004;Monaci et al, 2004;Pena et al, 2005).…”