Determination of Ochratoxin A in wine by packed in-tube solid phase microextraction followed by high performance liquid chromatography coupled to tandem mass spectrometry

Andrade, Mariane Aissa; Lanças, Fernando Mauro

doi:10.1016/j.chroma.2017.02.053

Cited by 84 publications

(49 citation statements)

References 13 publications

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“…This weaker attachment of the aptamer to the surface favors the aptamer–OTA interaction, the displacement of the aptamer, and cargo delivery. However, both LOD values comply with those established by the legislation and are similar to or better than most published techniques for the detection of OTA (see Table S4) …”

Section: Resultssupporting

confidence: 83%

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

et al. 2017

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Aptamers have been used as recognition elements for several molecules due to their great affinity and selectivity. Additionally, mesoporous nanomaterials have demonstrated great potential in sensing applications. Based on these concepts, we report herein the use of two aptamer‐capped mesoporous silica materials for the selective detection of ochratoxin A (OTA). A specific aptamer for OTA was used to block the pores of rhodamine B‐loaded mesoporous silica nanoparticles. Two solids were prepared in which the aptamer capped the porous scaffolds by using a covalent or electrostatic approach. Whereas the prepared materials remained capped in water, dye delivery was selectively observed in the presence of OTA. The protocol showed excellent analytical performance in terms of sensitivity (limit of detection: 0.5–0.05 nm), reproducibility, and selectivity. Moreover, the aptasensors were tested for OTA detection in commercial foodstuff matrices, which demonstrated their potential applicability in real samples.

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Section: Resultssupporting

confidence: 83%

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

et al. 2017

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“…Andrade et al. developed a sensitive online IT‐SPME system that utilized particle packed SPME column with a column switching interface to determine Ochratoxin A in wine, and the LODs and LOQs reached 0.02 and 0.05 μg/L respectively .…”

Section: Online Sample Preparation‐uhplc Techniquesmentioning

confidence: 99%

Recent advances of online coupling of sample preparation techniques with ultra high performance liquid chromatography and supercritical fluid chromatography

Liang

Zhou

2018

J of Separation Science

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Ultra high performance liquid chromatography and supercritical fluid chromatography techniques are favored because of their high efficiency and fast analysis speed. Although many sample preparation techniques have been coupled with common liquid chromatography online, the online coupling of sample preparation with the two popular chromatography techniques have gained increasing attention owing to the increasing requirements of efficiency and sensitivity. In this review, we have discussed and summarized the recent advances of the online coupling of sample preparation with ultra high performance liquid chromatography and supercritical fluid chromatography techniques. The main sample preparation techniques that have been coupled with ultra high performance liquid chromatography online are solid-phase extraction and in-tube solid-phase microextraction, while solid-phase extraction and supercritical fluid extraction are the main techniques that have been coupled with supercritical fluid chromatography online. Especially, the strategies for online coupling of sample preparation with chromatography techniques were summarized. Typical applications and growing trends of the online coupling techniques were also discussed in detail. With the increasing demands of improving the efficiency, throughput, and analytical capability toward complex samples of the analysis methods, online coupling of sample preparation with chromatography techniques will acquire further development.

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“…Mycotoxin contamination can occur at any stage during food production, including cultivation and storage (Reddy et al, 2010). For example, wine, beer, cereals, nuts, dried fruits, coffee, spices, beans, and beverages may become contaminated during their preparation (Andrade & Lancas, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Even though it is difficult to completely eliminate mycotoxins from foods, it is possible to decrease the risk of exposure via a rigorous monitoring programme. In Brazil and in the European Union, the maximum permissible level of OTA in wine is 2 µg/kg (Andrade & Lancas, 2017;Zhu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine

Liu

Suryoprabowo

et al. 2017

Food and Agricultural Immunology

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Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatographic assay for detection of OTA in 0.01 M phosphate-buffered saline solution (PBS) (pH 7.4) and in red wine. We produced sensitive and selective monoclonal antibody against OTA. Under optimized conditions, the cut-off limit of the test strip was 10 ng/mL OTA in 0.01 M PBS (pH 7.4) and in red wine. The results were obtained within 10 min. Therefore, our developed method is useful for the detection of OTA in red wine. ARTICLE HISTORY

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Determination of Ochratoxin A in wine by packed in-tube solid phase microextraction followed by high performance liquid chromatography coupled to tandem mass spectrometry

Cited by 84 publications

References 13 publications

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

Recent advances of online coupling of sample preparation techniques with ultra high performance liquid chromatography and supercritical fluid chromatography

Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine

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