Determination of okadaic acid in shellfish by using a novel chemiluminescent enzyme-linked immunosorbent assay method

Vdovenko, Marina M.; Hung, Chun-Tse; Sakharov, Ivan Yu.; Yu, Feng‐Yih

doi:10.1016/j.talanta.2013.05.057

Cited by 28 publications

(6 citation statements)

References 25 publications

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“…However, this assay is not truly rapid (i.e. TAT of less than 1 h) and requires numerous reagent additions and wash steps, incubations, trained personnel and dedicated equipment for the measurement of results [ 26 – 29 ]. Other systems for toxin detection use the same competitive format principle, but have improved read-out or incorporate high-density spotting in a microarray format [ 30 , 31 ].…”

Section: Detection Of Marine Biotoxins In Shellfishmentioning

confidence: 99%

Use of biosensors for the detection of marine toxins

McPartlin

Lochhead

Connell

et al. 2016

Essays in Biochemistry

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Increasing occurrences of harmful algal blooms (HABs) in the ocean are a major concern for countries around the globe, and with strong links between HABs and climate change and eutrophication, the occurrences are only set to increase. Of particular concern with regard to HABs is the presence of toxin-producing algae. Six major marine biotoxin groups are associated with HABs. Ingestion of such toxins via contaminated shellfish, fish, or other potential vectors, can lead to intoxication syndromes with moderate to severe symptoms, including death in extreme cases. There are also major economic implications associated with the diverse effects of marine biotoxins and HABs. Thus, effective monitoring programmes are required to manage and mitigate their detrimental global effect. However, currently legislated detection methods are labour-intensive, expensive and relatively slow. The growing field of biosensor diagnostic devices is an exciting area that has the potential to produce robust, easy-to-use, cost-effective, rapid and accurate detection methods for marine biotoxins and HABs. This review discusses recently developed biosensor assays that target marine biotoxins and their microbial producers, both in harvested fish/shellfish samples and in the open ocean. The effective deployment of such biosensor platforms could address the pressing need for improved monitoring of HABs and marine biotoxins, and could help to reduce their global economic impact.

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Section: Detection Of Marine Biotoxins In Shellfishmentioning

confidence: 99%

Use of biosensors for the detection of marine toxins

McPartlin

Lochhead

Connell

et al. 2016

Essays in Biochemistry

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“…Currently, LC-MS/MS has been established as the official EU detection method [ 22 ]. The immunoassay technique uses the specific binding between antigen and antibody to detect the analyte, which has high specificity, high sensitivity, and wide application; thus, it is an important technical method for the detection of shellfish toxin OA [ 23 , 24 ]. The commonly used screening detection methods include ELISA, time-resolved fluorescence immunoassay (TRFIA), and other similar techniques; ELISA has been widely used in the study of OA.…”

Section: Introductionmentioning

confidence: 99%

Okadaic Acid Detection through a Rapid and Sensitive Amplified Luminescent Proximity Homogeneous Assay

Qin

Kuang

et al. 2023

Toxins

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Okadaic acid (OA), a marine biotoxin produced by microalgae, poses a significant threat to mariculture, seafood safety, and human health. The establishment of a novel, highly sensitive detection method for OA would have significant practical and scientific implications. Therefore, the purpose of this study was to develop an innovative approach for OA detection. A competitive amplified luminescent proximity homogeneous assay (AlphaLISA) was developed using the principle of specific antigen–antibody binding based on the energy transfer between chemiluminescent microspheres. The method was non-washable, sensitive, and rapid, which could detect 2 × 10−2–200 ng/mL of OA within 15 min, and the detection limit was 4.55 × 10−3 ng/mL. The average intra- and inter-assay coefficients of variation were 2.54% and 6.26%, respectively. Detection of the actual sample results exhibited a good correlation with high-performance liquid chromatography. In conclusion, a simple, rapid, sensitive, and accurate AlphaLISA method was established for detecting OA and is expected to significantly contribute to marine biotoxin research.

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“…The drawbacks of this method are the requirements for highly qualified personnel, enormous sample cleanup, and costly equipment. Recently, immunological analysis methods have been investigated as emerging screening tools for OA because they are rapid, simple and cost effective [ 15 , 16 , 17 , 18 ]. Nevertheless, the preparation of the monoclonal antibody is very expensive and time consuming, and the procedure requires the continued use of animals.…”

Section: Introductionmentioning

confidence: 99%

Generation of Internal-Image Functional Aptamers of Okadaic Acid via Magnetic-Bead SELEX

et al. 2015

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Okadaic acid (OA) is produced by Dinophysis and Prorocentrum dinoflagellates and primarily accumulates in bivalves, and this toxin has harmful effects on consumers and operators. In this work, we first report the use of aptamers as novel non-toxic probes capable of binding to a monoclonal antibody against OA (OA-mAb). Aptamers that mimic the OA toxin with high affinity and selectivity were generated by the magnetic bead-assisted systematic evolution of ligands by exponential enrichment (SELEX) strategy. After 12 selection rounds, cloning, sequencing and enzyme-linked immunosorbent assay (ELISA) analysis, four candidate aptamers (O24, O31, O39, O40) were selected that showed high affinity and specificity for OA-mAb. The affinity constants of O24, O31, O39 and O40 were 8.3 × 108 M−1, 1.47 × 109 M−1, 1.23 × 109 M−1 and 1.05 × 109 M−1, respectively. Indirect competitive ELISA was employed to determine the internal-image function of the aptamers. The results reveal that O31 has a similar competitive function as free OA toxin, whereas the other three aptamers did not bear the necessary internal-image function. Based on the derivation of the curvilinear equation for OA/O31, the equation that defined the relationship between the OA toxin content and O31 was Y = 2.185X − 1.78. The IC50 of O31 was 3.39 ng·mL−1, which was close to the value predicted by the OA ELISA (IC50 = 4.4 ng·mL−1); the IC10 was 0.33 ng·mL−1. The above data provides strong evidence that internal-image functional aptamers could be applicable as novel probes in a non-toxic assay.

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Determination of okadaic acid in shellfish by using a novel chemiluminescent enzyme-linked immunosorbent assay method

Cited by 28 publications

References 25 publications

Use of biosensors for the detection of marine toxins

Use of biosensors for the detection of marine toxins

Okadaic Acid Detection through a Rapid and Sensitive Amplified Luminescent Proximity Homogeneous Assay

Generation of Internal-Image Functional Aptamers of Okadaic Acid via Magnetic-Bead SELEX

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