Determination of olanzapine in human blood by liquid chromatography–tandem mass spectrometry

Berna, Michael J.; Ackermann, Brad; Ruterbories, Kenneth J.; Glass, S. J.

doi:10.1016/s0378-4347(01)00548-5

Cited by 57 publications

(58 citation statements)

References 7 publications

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“…However, as we previously demonstrated in our extensively validated serum method, olanzapine can be quantified reliably without instability problems when no water was used to reconstitute the extract before analysis [21]. Still, it is known that olanzapine is more unstable in whole blood than in serum or plasma [20,27,29].…”

Section: Comparison Between Serum and Whole Bloodmentioning

confidence: 86%

“…For olanzapine, the difference was even more pronounced (calculated blood:serum ratio 1.2 vs. published blood:plasma ratio 0.6) [25]. Olanzapine is known to be a difficult compound with frequently reported instability in an aqueous environment [11,27,28]. However, as we previously demonstrated in our extensively validated serum method, olanzapine can be quantified reliably without instability problems when no water was used to reconstitute the extract before analysis [21].…”

Section: Comparison Between Serum and Whole Bloodmentioning

confidence: 92%

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Are Capillary DBS Applicable for Therapeutic Drug Monitoring of Common Antipsychotics? A Proof of Concept

et al. 2015

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Aim: DBS sampling has been proposed as an alternative for venous blood collection in therapeutic drug monitoring (TDM) of antipsychotics. For implementation in routine practice, a comparison between capillary and venous blood concentrations is mandatory. Results: A DBS method for quantification of antipsychotics was clinically validated. First, whole blood therapeutic ranges were calculated using the blood:serum ratio. Calculation of DBS:blood ratios and Passing–Bablok regression analysis demonstrated that concentrations obtained by DBS analysis were highly comparable to those obtained by conventional whole blood analysis. Clinical interpretation of serum, whole blood and DBS concentrations were highly identical (sensitivity 91.6–97.6%). Conclusion: This is the first clinical study demonstrating the value of DBS sampling in TDM of antipsychotics.

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Section: Comparison Between Serum and Whole Bloodmentioning

confidence: 86%

Section: Comparison Between Serum and Whole Bloodmentioning

confidence: 92%

Are Capillary DBS Applicable for Therapeutic Drug Monitoring of Common Antipsychotics? A Proof of Concept

et al. 2015

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“…Several analytical methods have been reported for the determination of OLP in biological fluids and in pharmaceutical preparations, and they include high performance liquid chromatography (HPLC) with ultraviolet (Olesen et al, 1998;Boulton et al, 2001;Harald et al, 2001;Concetta et al, 2006;Dusci et al, 2002;Olesen et al, 2001;Saracino et al, 2007;Shah et al, 2007;Reddy et al, 2007;Xuejun et al, 2004); electrochemical (Saracino et al, 2006;Bao, Potts., 2001;Kasper et al, 1999) or mass spectrometric (Thieme, Sachs., 2003;Berna et al, 2002;Titier et al, 2003;Dusci et al, 2002) detection, gas chromatography (Sabbioni et al, 2004), capillary zone electrophoresis , UVspectrophotometry and non-aqueous titrimetry (Firdous et al, 2005). However, there are only three reports on the visible spectrophotometric methods (Jasinska, Nalewajko, 2004;Mohamed, 2008;Krebs et al, 2006) based on redox reaction using hexacyanoferrate (III), cerium (IV), Nbromosuccinimide (NBS) or potassium iodate.…”

Section: Introductionmentioning

confidence: 99%

Determination of olanzapine by spectrophotometry using permanganate

Rajendraprasad

Basavaiah

2009

Braz. J. Pharm. Sci.

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Two new spectrophotometric methods using permanganate as the oxidimetric reagent for the determination of olanzapine (OLP) were developed and validated as per the current ICH guidelines. The methods involved the addition of known excess of permanganate to OLP in either acid or alkaline medium followed by the determination of unreacted permanganate at 550 nm (method A) or bluish-green color of manganate at 610 nm (method B). The decrease in absorbance in method A or increase in absorbance in method B as a function of concentration of OLP was measured and related to OLP concentration. Under optimized conditions, Beer's law was obeyed over the ranges 2.0 to 20 and 1.0 to 10 µg mL . The LOD and LOQ for method A were calculated to be 0.37 and 1.13 µg mL -1 and the corresponding values for method B were 0.16 and 0.48 µg mL -1 . Intermediate precision, expressed as RSD was in the range 0.51 to 2.66 %, and accuracy, expressed as relative error ranged from 0.79 to 2.24 %. The proposed methods were successfully applied to the assay of OLP in commercial tablets with mean percentage recoveries of 102 ±1.59 % (method A) and 101 ±1.53 % (method B). The accuracy and reliability of the methods were further confirmed by performing recovery tests via standard addition procedure.Uniterms: Olanzapine/determination. Spectrophotometry. Permanganate. Pharmaceuticals. Dois métodos espectrofotométricos novos, usando o permanganato como o reagente oxidimétrico para a determinação da olanzapina (OLP) foram utilizados e validados de acordo com as diretrizes atuais do ICH. Os métodos envolveram a adição de excesso conhecido de permanganato à OLP em meio ácido ou alcalino, determinando-se o permanganato que não reagiu em 550 nm (método A), ou pela cor verdeazulada do manganato a 610 nm (método B). A diminuição da absorbância no método A ou o aumento da absorbância no método B, em função da concentração de OLP, foi medida e relacionada à concentração de OLP. Sob condições otimizadas, a lei de Beer foi obedecida, nas faixas de concentração de 2,0 a 20 e 1,0 a 10 ao µg mL-1, no método A e no método B, respectivamente. Os valores de absortividade molar foram de 1,34 x 104 e 2,54 x 104 l mol-1cm-1 para o método A e para o método B, respectivamente, e as sensibilidades respectivas de Sandell foram de 0,0233 e 0,0123 µg cm-2. Os LOD e os LOQ para o método A calculados foram 0,37 e 1,13 mg mL-1e os valores correspondentes para o método B foram 0,16 e 0,48 µg mL-1. A precisão intermediária, expressa como RSD, encontrou-se na faixa de 0,51 a 2,66%, e a exatidão, expressa como o erro relativo, variou de 0,79 a 2,24%. Os métodos propostos foram aplicados com sucesso ao ensaio de OLP em comprimidos comerciais, com porcentagens médias de recuperação de 102± 1,59% (método A) e de 101± 1,53% (método B). A exatidão e a confiabilidade dos métodos foram confirmadas executando testes de recuperação através de procedimento padrão de adição.Uniterms: Olanzapina/determinação. Espectrofotometria. Permanganato. Produtos farmacêuticos.

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“…Some LLE methods for OLZ, HAL, CLOZ and CPZ have been reported (Gervasini et al, 2003;Nirogi et al, 2006;Hattori et al, 1986;Yasui-Furukori et al, 2004;McKay et al, 1982;Li et al, 2006;Midha et al, 1981;Zhang et al, 2007;Garay Garcia et al, 2003;Ho et al, 2004;Chung et al, 1993). Berna et al (2002) described an LC-MS/MS method for the determination of OLZ from whole blood. Liquid-liquid extraction, using nbutanol:cyclohexane (3:47, v/v), was used to isolate OLZ from 250 μL of whole blood instead of using a more traditional SPE approach.…”

Section: Liquid-liquid Extractionmentioning

confidence: 99%

Bioanalytical methods for the determination of antipsychotic drugs

Zhang

Terry

Bartlett

2008

Biomedical Chromatography

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Antipsychotic drugs are popular for the treatment of schizophrenia and other psychoses. In general, antipsychotics are administered in oral doses of only a few milligrams per day and they are widely metabolized in the body. Therefore, the concentration of these drugs in plasma is very low (pg-ng/mL levels). In addition, therapeutic drug monitoring (TDM) of antipsychotic drugs has proven to be of notable value for determining poor compliance of patients and addressing the challenges associated with considerable genetic variability in their metabolism. Thus, in order to conduct pharmacology and toxicology studies and clinical TDM of antipsychotics, as well as address the challenges associated with polypharmacy and drug metabolism, highly sensitive, selective and accurate bioanalytical methods are essential. The most recent studies on the determination of antipsychotics will be reviewed, including separation techniques, sample pretreatment, detectors and validation.

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Determination of olanzapine in human blood by liquid chromatography–tandem mass spectrometry

Cited by 57 publications

References 7 publications

Are Capillary DBS Applicable for Therapeutic Drug Monitoring of Common Antipsychotics? A Proof of Concept

Are Capillary DBS Applicable for Therapeutic Drug Monitoring of Common Antipsychotics? A Proof of Concept

Determination of olanzapine by spectrophotometry using permanganate

Bioanalytical methods for the determination of antipsychotic drugs

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